Invitrogen

JIP1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

3 Published Figures

4 References

View all (18) JIP1 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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JIP1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of JIP 1 (N-TERM) was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with JIP 1 Rabbit Polyclonal Antibody (Product # 34-5200) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

JIP1 Antibody in Western Blot (WB) — Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Mouse Adipose (Lane 2), Rat Adipose (Lane 3), Rat Kidney (Lane 4), Rat Liver (Lane 5), Mouse Stomach (Lane 6) and Rat Stomach (Lane 7). The blot was probed with Anti- JIP1 Rabbit Polyclonal Antibody (Product # 34-5200, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 100 kDa band corresponding to JIP1 was observed in Mouse Brain and not observed in other tissues which are documented to be JIP1 negative.

JIP1 Antibody in Immunohistochemistry (IHC) — Published figure using JIP1 polyclonal antibody (Product # 34-5200) in Immunohistochemistry

JIP1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue models owing to their inherent genetic constitution. Relative expression of JIP1 was observed in Mouse Brain, Mouse Adipose, Rat Adipose, Rat Kidney, Rat Liver, Mouse Stomach and Rat Stomach in Western Blot using JIP1 Polyclonal Antibody (Product # 34-5200). JIP1 is reported to be expressed in brain tissue and not in other tissues like adipose, kidney, liver and stomach.

Product Details

34-5200

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the N-terminal region of the human JIP1 protein.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

RRID

AB_2533173

Target Information

The protein encoded by this gene is a regulator of the pancreatic beta-cell function. It is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). This protein has been shown to prevent MAPK8 mediated activation of transcription factors, and decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2. RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells. This gene is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Phosphorylated Tau interacts with c-Jun N-terminal kinase-interacting protein 1 (JIP1) in Alzheimer disease.

The Journal of biological chemistry

Ittner LM,Ke YD,Götz J

Fri Jul 31 00:00:00 EDT 2009

Phosphorylated Tau interacts with c-Jun N-terminal kinase-interacting protein 1 (JIP1) in Alzheimer disease.

The Journal of biological chemistry

Ittner LM,Ke YD,Götz J

Fri Jul 31 00:00:00 EDT 2009

Phosphorylated Tau interacts with c-Jun N-terminal kinase-interacting protein 1 (JIP1) in Alzheimer disease.

The Journal of biological chemistry

Ittner LM,Ke YD,Götz J

Fri Jul 31 00:00:00 EDT 2009

Jun NH2-terminal kinase (JNK) interacting protein 1 (JIP1) binds the cytoplasmic domain of the Alzheimer's beta-amyloid precursor protein (APP).

The Journal of biological chemistry

Scheinfeld MH,Roncarati R,Vito P,Lopez PA,Abdallah M,D'Adamio L

Fri Feb 01 00:00:00 EST 2002

Bioinformatics

Protein Aliases: C-Jun-amino-terminal kinase-interacting protein 1; IB-1; Islet-brain 1; Islet-brain-1; JIP-1; JIP-1-related protein; JNK MAP kinase scaffold protein 1; JNK-interacting protein 1; JRP; mitogen activated protein kinase 8 interacting protein; mitogen activated protein kinase 8 interacting protein 1; Mitogen-activated protein kinase 8-interacting protein 1; PRKM8 interacting protein; protein kinase, mitogen-activated 8 interacting protein

Gene Aliases: IB1; JIP-1; JIP1; Mapk8ip; MAPK8IP1; mjip-2a; PRKM8IP; Skip

UniProt ID: (Human) Q9UQF2, (Rat) Q9R237, (Mouse) Q9WVI9

Entrez Gene ID: (Human) 9479, (Rat) 116457, (Mouse) 19099

Molecular Function: scaffold/adaptor protein