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Invitrogen
JMJD1A Polyclonal Antibody
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FIGURE: 1 / 4
JMJD1A Antibody (PA5-23066) in ICC/IF
Immunocytochemistry analysis of JMJD1A in MCF-7 cells. Samples were incubated with JMJD1A polyclonal antibody (Product # PA5-23066) followed by Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Product Details
PA5-23066
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A genomic peptide made to an internal region of the human JMJD1A protein (within residues 250-400).
Conjugate
Form
Liquid
Concentration
0.53 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 30% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The target sequence has 91% sequence homology with rat.
Target Information
JMJD1A is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. JMJD1A is involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. It's also Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: [histone H3]-dimethyl-L-lysine(9) demethylase 3A; DKFZp686A24246; DKFZp686P07111; JmjC domain-containing histone demethylation protein 2A; jumonji C domain-containing histone demethylase 2A; jumonji domain containing 1A; Jumonji domain-containing protein 1A; lysine (K)-specific demethylase 3A; Lysine-specific demethylase 3A; probable zinc finger protein; testis specific gene A; Testis-specific gene A protein; testis-specific protein A; Zinc finger protein TSGA
Gene Aliases: 1700105C21Rik; C230043E16Rik; JHDM2A; JHMD2A; JMJD1; JMJD1A; KDM2A; KDM3A; KIAA0742; TGSA; TSGA
UniProt ID: (Human) Q9Y4C1, (Mouse) Q6PCM1, (Rat) Q63679
Entrez Gene ID: (Human) 55818, (Mouse) 104263, (Rat) 312440
transcription regulatory region sequence-specific DNA binding
core promoter binding
transcription factor activity, sequence-specific DNA binding
iron ion binding
histone demethylase activity
androgen receptor binding
dioxygenase activity
sulfonate dioxygenase activity
protein binding
oxidoreductase activity
2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity
procollagen-proline dioxygenase activity
hypophosphite dioxygenase activity
DNA-N1-methyladenine dioxygenase activity
metal ion binding
C-19 gibberellin 2-beta-dioxygenase activity
C-20 gibberellin 2-beta-dioxygenase activity
molecular_function
histone modifying enzyme
response to hypoxia
transcription, DNA-templated
spermatid nucleus elongation
male gonad development
hormone-mediated signaling pathway
androgen receptor signaling pathway
histone H3-K9 demethylation
histone H3-K9 dimethylation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
formaldehyde biosynthetic process
negative regulation of histone H3-K9 methylation
oxidation-reduction process
regulation of stem cell population maintenance
regulation of stem cell differentiation
regulation of transcription, DNA-templated
spermatogenesis
positive regulation of gene expression
chromatin modification
cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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