|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide made to an internal region of the human JMJD2C protein (within residues 450-600).|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:10000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
JMJD2C is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein with one JmjC domain, one JmjN domain, two PHD-type zinc fingers, and two Tudor domains. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form. Chromosomal aberrations and increased transcriptional expression of this gene are associated with esophageal squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
PITX1, a specificity determinant in the HIF-1¿-mediated transcriptional response to hypoxia.
PA5-23065 was used in western blot to identify PITX1 as a key specificity factor for HIF-1alpha dependent gene expression.
|Mudie S,Bandarra D,Batie M,Biddlestone J,Moniz S,Ortmann B,Shmakova A,Rocha S||Cell cycle (Georgetown, Tex.) (13:3878)||2015|