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Invitrogen
JMJD3 Polyclonal Antibody
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FIGURE: 1 / 4
JMJD3 Antibody (PA5-22974) in ICC/IF
Immunocytochemistry analysis of JMJD3 in HeLa cells. Samples were incubated with JMJD3 polyclonal antibody (Product # PA5-22974) using a dilution of 1:200 dilution overnight at 4°C followed by anti-rabbit Dylight 488 (Green) at a dilution of 1:500. Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. Alpha tubulin (DM1A) was used as a co-stain at a 1:1,000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (... View More
Product Details
PA5-22974
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide made to an internal portion of the mouse JMJD3 protein (within residues 600-650).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The target sequence has 100% sequence homology with rat.
Target Information
Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: [histone H3]-trimethyl-L-lysine(27) demethylase 6B; JmjC domain-containing protein 3; jumonji domain containing 3; jumonji domain containing 3, histone lysine demethylase; Jumonji domain-containing protein 3; lysine (K)-specific demethylase 6B; Lysine demethylase 6B; Lysine-specific demethylase 6B
Gene Aliases: 1700064E03Rik; BC038313; JMJD3; KDM6B; KIAA0346; mKIAA0346
UniProt ID: (Human) O15054, (Mouse) Q5NCY0
Entrez Gene ID: (Human) 23135, (Mouse) 216850
chromatin binding
protein binding
beta-catenin binding
histone demethylase activity
sequence-specific DNA binding
metal ion binding
dioxygenase activity
histone demethylase activity (H3-K27 specific)
sulfonate dioxygenase activity
oxidoreductase activity
2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity
procollagen-proline dioxygenase activity
hypophosphite dioxygenase activity
DNA-N1-methyladenine dioxygenase activity
C-19 gibberellin 2-beta-dioxygenase activity
C-20 gibberellin 2-beta-dioxygenase activity
chromatin/chromatin-binding, or -regulatory protein
inflammatory response to antigenic stimulus
response to activity
hippocampus development
cell fate commitment
endothelial cell differentiation
positive regulation of transcription from RNA polymerase II promoter
mesodermal cell differentiation
cardiac muscle cell differentiation
oxidation-reduction process
response to fungicide
cellular response to hydrogen peroxide
histone H3-K27 demethylation
inflammatory response
chromatin modification
histone demethylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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