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JNK Polyclonal Antibody

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JNK Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Acetone ) fixed SH-SY5Y cells using 51151-1-AP (JNK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

JNK Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 51151-1-AP (JNK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

JNK Antibody in Immunoprecipitation (IP) — IP result of anti-JNK (IP:51151-1-AP, 4ug; Detection:51151-1-AP 1:600) with HEK-293 cells lysate 2000ug.

Product Details

51151-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 52 publications 52 publications
Immunohistochemistry (IHC)	Assay-dependent	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1:50-1:500	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 4 publications 4 publications

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Mouse, Plant, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide
Conjugate	Unconjugated
Form	Liquid
Concentration	0.19 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

The MAPK8 protein can be phosphorylated and this antibody recognizes the 46 kDa and 55 kDa bands in western blot.

Target Information

C-Jun N-terminal Kinase (JNK) is also known as Mitogen-activated protein kinase (MAPK8). It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. JNK is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Bioinformatics

Protein Aliases: c-Jun kinase 2; c-Jun N-terminal kinase 1; c-Jun N-terminal kinase 2; c-Jun N-terminal kinase 3; FLJ12099; FLJ33785; JNK 46; JNK 55; JNK-46; JNK-55; JNK/SAPK alpha; JNK1 beta1 protein kinase; JNK3 alpha protein kinase; JNK3 beta1 protein kinase; JNK3 beta2 protein kinase; Jun kinase; JUN N-terminal kinase; MAP kinase 10; MAP kinase 8; MAP kinase 9; MAP kinase p49 3F12; MAPK 10; MAPK 8; MAPK 9; MGC50974; Mi; mitogen activated protein kinase 10; mitogen activated protein kinase 8; mitogen activated protein kinase 9; Mitogen-activated protein kinase 10; Mitogen-activated protein kinase 8; mitogen-activated protein kinase 8 isoform JNK1 alpha1; mitogen-activated protein kinase 8 isoform JNK1 beta2; Mitogen-activated protein kinase 9; MK10; Phospho-JNK; protein kinase mitogen-activated 8; protein kinase, mitogen-activated 9; SAPK/Erk/kinase 2; SAPK1a; SAPK1b; SAPK1c; stress activated protein kinase beta; stress-activated protein kinase 1; Stress-activated protein kinase 1a; Stress-activated protein kinase 1b; Stress-activated protein kinase 1c; Stress-activated protein kinase JNK1; Stress-activated protein kinase JNK2; Stress-activated protein kinase JNK3

Gene Aliases: AI849689; AI851083; C230008H04Rik; JNK; JNK-46; JNK-55; JNK1; JNK1A2; JNK2; JNK21B1/2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; JNK3; JNK3A; JNK3B1; JNK3B2; MAPK10; MAPK8; MAPK9; p493F12; p54a; p54aSAPK; p54bSAPK; PRKM10; PRKM8; PRKM9; SAPK; SAPK(beta); SAPK1; SAPK1A; SAPK1B; SAPK1C; Serk2

UniProt ID: (Human) P53779, (Human) P45983, (Human) P45984, (Mouse) Q61831, (Mouse) Q91Y86, (Mouse) Q9WTU6

Entrez Gene ID: (Human) 5602, (Human) 5599, (Human) 5601, (Mouse) 26414, (Mouse) 26419, (Mouse) 26420

Molecular Function: non-receptor serine/threonine protein kinase