Invitrogen

JPH1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

15 Published Figures

14 References

View all (7) JPH1 antibodies

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Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

JPH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Junctophilin-1 showing staining in the cytoplasm of paraffin-embedded mouse skeletal muscle tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- Junctophilin-1 Polyclonal Antibody (Product # 40-5100) diluted in 3% BSA-PBS at a dilution of 1:50 overnight at 4ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

JPH1 Antibody in Immunohistochemistry (IHC) — Immunofluorescent staining of mouse hippocampal dentate gyrus using Junctophilin-1 (C-term) Polyclonal Antibody, Rabbit (Product # 40-5100). Image courtesy of James I. Nagy, PhD, University of Manitoba, Canada

JPH1 Antibody in Western Blot (WB) — Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Muscle (Lane 1), and Mouse Heart (lane 2). The blots were probed with Anti-Junctophilin-1 Rabbit Polyclonal Antibody (Product # 40-5100, 2 µg/mL) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A 70 kDa band corresponding to Junctophilin-1 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane by iBlot® 2 Dry Blotting System (Product # IB21001).The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

JPH1 Antibody — Figure 3 Proteolysis of JP1 in intensively stimulated muscles A , Western blot of JP1 in fibres from rat EDL muscles stimulated intensively in vitro in the presence of 30 m m caffeine (Stim) and from non-stimulated contralateral muscles (Con); three fibre segments were pooled in each lane. Proteins were separated on 4-15% Stain Free gels and probed for JP1 using mid-region or C-terminal antibody, and also antibody to RyR1. B , mean (+SEM) amount of 75 kDa JP1 fragment in fibre samples from Stim and Con muscles, expressed as a percentage of the total JP1 detected (i.e. the sum of the 75 and 90 kDa bands). The number of samples is shown with each bar, together with the number of muscles in parentheses (** P < 0.01, Mann-Whitney U test). C , mean (+SEM) amount of 15 kDa JP1 fragment in samples (each value was first normalized to MHC). D , mean (+SEM) amount of RyR1 in samples. E , Western blot of JP1 and the 75 and 15 kDa proteolytic fragments remaining in skinned fibre (F-sk) or diffusing into wash solution (Diff) after bathing skinned fibre segments in wash solution for 5 min (see Methods). Fibres were obtained from stimulated muscle; three skinned segments were used in each of two wash-fibre sets shown. A substantial proportion of the 75 kDa proteolytic fragments diffused into the wash solution (arrows). {TM}

Product Details

40-5100

Applications	Tested Dilution	Publications
Western Blot (WB)	2-3 µg/mL	View 7 publications 7 publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50	View 1 publication 1 publication
Immunohistochemistry (Frozen) (IHC (F))	Assay-dependent	-
Immunocytochemistry (ICC/IF)	-	View 2 publications 2 publications
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse, Rat
Published species	Human, Mouse, Rabbit, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide derived from the C-terminal region of the mouse junctophilin-1 (JP-1, junctophilin type 1, JPH1) protein, which differs from rat by one non-conservative amino acid replacement View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.25 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4
Contains	0.1% sodium azide
Storage conditions	-20°C
RRID	AB_2533470

Target Information

Junctional complexes between the plasma membrane and endoplasmic/sarcoplasmic reticulum are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. The protein encoded by this gene is a component of junctional complexes and is composed of a C-terminal hydrophobic segment spanning the endoplasmic/sarcoplasmic reticulum membrane and a remaining cytoplasmic domain that shows specific affinity for the plasma membrane. This gene is a member of the junctophilin gene family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

l-arginine ingestion inhibits eccentric contraction-induced proteolysis and force deficit via S-nitrosylation of calpain.

Physiological reports

Kanzaki K,Watanabe D,Aibara C,Kawakami Y,Yamada T,Takahashi Y,Wada M

Published figure using JPH1 polyclonal antibody (Product # 40-5100) in Western Blot

Mon Jan 01 00:00:00 EST 2018

l-arginine ingestion inhibits eccentric contraction-induced proteolysis and force deficit via S-nitrosylation of calpain.

Physiological reports

Kanzaki K,Watanabe D,Aibara C,Kawakami Y,Yamada T,Takahashi Y,Wada M

Published figure using JPH1 polyclonal antibody (Product # 40-5100) in Western Blot

Mon Jan 01 00:00:00 EST 2018

Role of calpain in eccentric contraction-induced proteolysis of Ca²⁺-regulatory proteins and force depression in rat fast-twitch skeletal muscle.

Journal of applied physiology (Bethesda, Md. : 1985)

Kanzaki K,Watanabe D,Kuratani M,Yamada T,Matsunaga S,Wada M

40-5100 was used in Western Blotting to study the in vivo effects of eccentric contraction on calpain-dependent proteolysis of Ca2+ regulatory proteins and force production in fast twitch skeletal muscles.

Wed Feb 01 00:00:00 EST 2017

Evaluation of adipose-derived stem cells for tissue-engineered muscle repair construct-mediated repair of a murine model of volumetric muscle loss injury.

International journal of nanomedicine

Kesireddy V

Published figure using JPH1 polyclonal antibody (Product # 40-5100) in Immunohistochemistry

Tue Oct 25 00:00:00 EDT 2016

Eps 15 Homology Domain (EHD)-1 Remodels Transverse Tubules in Skeletal Muscle.

PloS one

Demonbreun AR,Swanson KE,Rossi AE,Deveaux HK,Earley JU,Allen MV,Arya P,Bhattacharyya S,Band H,Pytel P,McNally EM

Published figure using JPH1 polyclonal antibody (Product # 40-5100) in Western Blot

Tue May 31 00:00:00 EDT 2016

Identification of Small Ankyrin 1 as a Novel Sarco(endo)plasmic Reticulum Ca2+-ATPase 1 (SERCA1) Regulatory Protein in Skeletal Muscle.

The Journal of biological chemistry

Desmond PF,Muriel J,Markwardt ML,Rizzo MA,Bloch RJ

40-5100 was used in western blot to test if sAnk and SERCA interact.

Fri Nov 13 00:00:00 EST 2015

Observation of the molecular organization of calcium release sites in fast- and slow-twitch skeletal muscle with nanoscale imaging.

Journal of the Royal Society, Interface

Jayasinghe ID,Munro M,Baddeley D,Launikonis BS,Soeller C

Published figure using JPH1 polyclonal antibody (Product # 40-5100) in Immunofluorescence

Mon Oct 06 00:00:00 EDT 2014

Oxysterols modulate calcium signalling in the A7r5 aortic smooth muscle cell-line.

Biochimie

Hammoud Y,Rice T,Mackrill JJ

40-5100 was used in immunocytochemistry to assess the effects of oxysterols on calcium signaling mechanisms in A7r5 cells.

Fri Mar 01 00:00:00 EST 2013

Ca2+-dependent proteolysis of junctophilin-1 and junctophilin-2 in skeletal and cardiac muscle.

The Journal of physiology

Murphy RM,Dutka TL,Horvath D,Bell JR,Delbridge LM,Lamb GD

40-5100 was used in western blot to study junctophilin proteolysis in skeletal muscle.

Fri Feb 01 00:00:00 EST 2013

The role of endothelial cells in myofiber differentiation and the vascularization and innervation of bioengineered muscle tissue in vivo.

Biomaterials

Criswell TL,Corona BT,Wang Z,Zhou Y,Niu G,Xu Y,Christ GJ,Soker S

40-5100 was used in immunohistochemistry to study the role of endothelial cells in myofiber differentiation and the development of bioengineered muscle tissue in vivo.

Tue Jan 01 00:00:00 EST 2013

Further development of a tissue engineered muscle repair construct in vitro for enhanced functional recovery following implantation in vivo in a murine model of volumetric muscle loss injury.

Tissue engineering. Part A

Corona BT,Machingal MA,Criswell T,Vadhavkar M,Dannahower AC,Bergman C,Zhao W,Christ GJ

40-5100 was used in immunohistochemistry - paraffin section and western blot to optimize tissue engineered muscle repair constructs.

Fri Jun 01 00:00:00 EDT 2012

Further development of a tissue engineered muscle repair construct in vitro for enhanced functional recovery following implantation in vivo in a murine model of volumetric muscle loss injury.

Tissue engineering. Part A

Corona BT,Machingal MA,Criswell T,Vadhavkar M,Dannahower AC,Bergman C,Zhao W,Christ GJ

40-5100 was used in immunohistochemistry - paraffin section and western blot to optimize tissue engineered muscle repair constructs.

Fri Jun 01 00:00:00 EDT 2012

Junctophilin 1 and 2 proteins interact with the L-type Ca2+ channel dihydropyridine receptors (DHPRs) in skeletal muscle.

The Journal of biological chemistry

Golini L,Chouabe C,Berthier C,Cusimano V,Fornaro M,Bonvallet R,Formoso L,Giacomello E,Jacquemond V,Sorrentino V

Fri Dec 23 00:00:00 EST 2011

Effect of prior exercise on thermal sensitivity of malignant hyperthermia-susceptible muscle.

Muscle & nerve

Corona BT,Hamilton SL,Ingalls CP

40-5100 was used in western blot to assess impact of eccentric and concentric exercise on the thermal sensitivity of isolated malignant hyperthermia-susceptible mouse muscle

Sun Aug 01 00:00:00 EDT 2010

Junctophilin damage contributes to early strength deficits and EC coupling failure after eccentric contractions.

American journal of physiology. Cell physiology

Corona BT,Balog EM,Doyle JA,Rupp JC,Luke RC,Ingalls CP

40-5100 was used in Western Blotting to indicate that Junctophilin damage plays a role in early force deficits due to excitation-contraction coupling failure following the performance of eccentric contractions.