Invitrogen

JPH1 Polyclonal Antibody

14 Published Figures

13 References

View all (7) JPH1 antibodies

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Datasheet

Questions & Answers

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JPH1 Antibody in Immunofluorescence (IF) — Immunofluorescent staining of mouse hippocampal dentate gyrus using Junctophilin-1 (C-term) Polyclonal Antibody, Rabbit (Product # 40-5100). Image courtesy of James I. Nagy, PhD, University of Manitoba, Canada

JPH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Junctophilin-1 showing staining in the cytoplasm of paraffin-embedded mouse skeletal muscle tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- Junctophilin-1 Polyclonal Antibody (Product # 40-5100) diluted in 3% BSA-PBS at a dilution of 1:50 overnight at 4ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

JPH1 Antibody in Western Blot (WB) — Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Muscle (Lane 1), and Mouse Heart (lane 2). The blots were probed with Anti-Junctophilin-1 Rabbit Polyclonal Antibody (Product # 40-5100, 2 µg/mL) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A 70 kDa band corresponding to Junctophilin-1 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane by iBlot® 2 Dry Blotting System (Product # IB21001).The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

JPH1 Antibody — Published figure using JPH1 polyclonal antibody (Product # 40-5100)

Product Details

Applications

Tested Dilution

Publications

Immunofluorescence (IF)

Assay Dependent

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay Dependent

Immunohistochemistry (Paraffin) (IHC (P))

1:50

View 1 publication 1 publication

Western Blot (WB)

2-3 µg/mL

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunocytochemistry (ICC)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Human, Mouse, Rabbit, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the C-terminal region of the mouse junctophilin-1 (JP-1, junctophilin type 1, JPH1) protein, which differs from rat by one non-conservative amino acid replacement

Conjugate

Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

RRID

AB_2533470

Target Information

Junctional complexes between the plasma membrane and endoplasmic/sarcoplasmic reticulum are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. The protein encoded by this gene is a component of junctional complexes and is composed of a C-terminal hydrophobic segment spanning the endoplasmic/sarcoplasmic reticulum membrane and a remaining cytoplasmic domain that shows specific affinity for the plasma membrane. This gene is a member of the junctophilin gene family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.