Invitrogen

JPH2 Polyclonal Antibody

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This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

15 Published Figures

18 References

View all (22) JPH2 antibodies

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FIGURE: 1 / 21

JPH2 Antibody (40-5300) in WB

Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), and Mouse Heart (lane 2). The blots were probed with Anti-Junctophilin-2 Rabbit Polyclonal Antibody (Product # 40-5300, 2 µg/mL) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A 70 kDa band corresponding to Junctophilin-2 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP03... View More

Product Image Gallery

JPH2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Junctophilin-2 showing staining in the cytoplasm and membrane of paraffin-embedded mouse small intestine tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- Junctophilin-2 Polyclonal Antibody (Product # 40-5300) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

JPH2 Antibody in Immunohistochemistry (IHC) — Immunofluorescent staining of mouse skeletal muscle tissue using Rb anti-Junctophilin-2 (C-term) (Product # 40-5300). Image courtesy of James I. Nagy, PhD, University of Manitoba, Canada.

JPH2 Antibody in Immunocytochemistry (ICC/IF) — Figure 8 Structural integrity of excitation-contraction (EC) coupling junctions before and after VAD therapy of a myocarditis patient. (A,B) Raw confocal images of fixed isolated cardiomyocytes from pre- and post-VAD of the patient presented in Figures 6 , 7 , co-stained for LTCC (red), RyR (green), and JPH2 (blue) and with DAPI (not shown). (C,D) Overlay of binary images for the EC coupling proteins shown in (A,B) , with magnifications of boxed regions. The cell surface, obtained from autofluorescence, is shown white, nuclei are shown white with black asterisk. Co-localizations of LTCC, JPH2, and RyR appear cyan, magenta, yellow, or white (see color legend). (E) Cardiomyocyte JPH2 cluster density (JPH2 density) of AVSD as reference and the Pre- and Post-VAD sample. (F) Fraction of LTCC clusters that were co-localized with both RyR and JPH2, as a measure of intact EC coupling junctions ( n = 10/9 cells for Pre/Post-VAD). * p < 0.05, ** p < 0.01, *** p < 0.001, unpaired, two-tailed t -test with Holm-Bonferroni multiple-comparison correction.

JPH2 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Fig. 2 Confocal microscopy of left ventricular myocardium from 21-year-old donor. Image stacks were deconvolved to account for anisotropic blurring and corrected for depth-dependent signal attenuation. Example cross-sectional images from 3D stack for a WGA, b anti-RyR, and c anti-JPH2 antibody labeling. WGA served as a marker of the extracellular spaces and sarcolemma including t-system. d Overlay of a - c . e - h Zoom-ins of region in a - d marked with box in d . WGA-associated signals describe cross-sections of t-tubules. RyR- and JPH2-associated signals were apparent in small regions reflecting the clustering of these proteins. Histograms of sarcolemmal distances to i intracellular sites, j RyR clusters, and k JPH2 clusters. l Bar graph of sarcolemmal distances with error bars identifying their standard deviation. Scale bar in a marks 20 um and applies to b - d . Scale bar in e marks 2 um and applies to f - h .

JPH2 Antibody — Effect of Cav-3 OE and TAC on Cav-3 and JPH-2 protein expression. (a) Left panel, exemplar western blots of Cav-3 (18 kDa) and GAPDH (37 kDa) in isolated myocyte lysates from three WT sham (lanes 1), three Cav-3 OE sham (lanes 2) and three Cav-3 OE TAC (lanes 3) mice; Right panel, densitometry analysis of Cav-3 western blots ( N = 5 animals in each group in duplicate), compared with previously published data (Bryant et al., ) showing the effect of TAC on Cav-3 expression in WT mice (left two bars). (b) Left panel, exemplar western blots of JPH-2 (95 kDa) and GAPDH (37 kDa); right panel, densitometry analysis of JPH-2 western blots ( N = 5 animals in each group in duplicate), compared with previously published data (Bryant et al., ) showing the effect of TAC on JPH-2 expression in WT mice (left two bars). The blots in the left panels are from the same gels which were stripped and re-probed for the different proteins and were therefore obtained sequentially (see Methods). Data in each group (WT or Cav-3 OE) in the right panels are expressed as a percentage of the mean of the WT data in that group. ** P < 0.01, between treatments for a given phenotype (WT or Cav-3 OE); ++ P < 0.01, +++P < 0.001 between phenotypes {TM}

Product Details

40-5300

Applications

Tested Dilution

Publications

Western Blot (WB)

2-3 µg/mL

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 5 publications 5 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the C-terminal region of the mouse junctophilin-2 (JPH2, JP-2, junctophilin type 2) protein, which differs from rat by one non-conservative amino acid

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

RRID

AB_2533471

Target Information

This gene encodes a protein with a leucine-rich repeat and a calponin homology domain. Polymorphism in this gene may be associated with susceptibililty to knee osteoarthritis. Alternative splicing results in multiple transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Calpain-2 specifically cleaves Junctophilin-2 at the same site as Calpain-1 but with less efficacy.

The Biochemical journal

Wang J,Ciampa G,Zheng D,Shi Q,Chen B,Abel ED,Peng T,Hall DD,Song LS

Published figure using JPH2 polyclonal antibody (Product # 40-5300) in Western Blot

Fri Oct 15 00:00:00 EDT 2021

Remodeling of t-system and proteins underlying excitation-contraction coupling in aging versus failing human heart.

NPJ aging and mechanisms of disease

Lyu Y,Verma VK,Lee Y,Taleb I,Badolia R,Shankar TS,Kyriakopoulos CP,Selzman CH,Caine W,Alharethi R,Navankasattusas S,Seidel T,Drakos SG,Sachse FB

Published figure using JPH2 polyclonal antibody (Product # 40-5300) in Immunohistochemistry (Frozen)

Fri May 28 00:00:00 EDT 2021

Remodeling of t-system and proteins underlying excitation-contraction coupling in aging versus failing human heart.

NPJ aging and mechanisms of disease

Lyu Y,Verma VK,Lee Y,Taleb I,Badolia R,Shankar TS,Kyriakopoulos CP,Selzman CH,Caine W,Alharethi R,Navankasattusas S,Seidel T,Drakos SG,Sachse FB

Published figure using JPH2 polyclonal antibody (Product # 40-5300) in Immunohistochemistry (Frozen)

Fri May 28 00:00:00 EDT 2021

Severe T-System Remodeling in Pediatric Viral Myocarditis.

Frontiers in cardiovascular medicine

Fiegle DJ,Schöber M,Dittrich S,Cesnjevar R,Klingel K,Volk T,Alkassar M,Seidel T

Published figure using JPH2 polyclonal antibody (Product # 40-5300) in Immunocytochemistry

Sat Feb 06 00:00:00 EST 2021

Metabolic Maturation Media Improve Physiological Function of Human iPSC-Derived Cardiomyocytes.

Cell reports

Feyen DAM,McKeithan WL,Bruyneel AAN,Spiering S,Hörmann L,Ulmer B,Zhang H,Briganti F,Schweizer M,Hegyi B,Liao Z,Pölönen RP,Ginsburg KS,Lam CK,Serrano R,Wahlquist C,Kreymerman A,Vu M,Amatya PL,Behrens CS,Ranjbarvaziri S,Maas RGC,Greenhaw M,Bernstein D,Wu JC,Bers DM,Eschenhagen T,Metallo CM,Mercola M

40-5300 was used in Western Blotting to describe maturation media designed to provide oxidative substrates adapted to the metabolic needs of human iPSC (hiPSC)-CMs.

Tue Jul 21 00:00:00 EDT 2020

Nanoscale coupling of junctophilin-2 and ryanodine receptors regulates vascular smooth muscle cell contractility.

Proceedings of the National Academy of Sciences of the United States of America

Pritchard HAT,Griffin CS,Yamasaki E,Thakore P,Lane C,Greenstein AS,Earley S

40-5300 was used in Immunocytochemistry to conclude that JPH2 maintains functional coupling between RyR2s and BK channels and is critically important for cerebral arterial function.

Tue Oct 22 00:00:00 EDT 2019

Cardiac-specific overexpression of caveolin-3 preserves t-tubular I_Ca during heart failure in mice.

Experimental physiology

Kong CHT,Bryant SM,Watson JJ,Roth DM,Patel HH,Cannell MB,James AF,Orchard CH

Published figure using JPH2 polyclonal antibody (Product # 40-5300) in Western Blot

Wed May 01 00:00:00 EDT 2019

Cardiac-specific overexpression of caveolin-3 preserves t-tubular I_Ca during heart failure in mice.

Experimental physiology

Kong CHT,Bryant SM,Watson JJ,Roth DM,Patel HH,Cannell MB,James AF,Orchard CH

Published figure using JPH2 polyclonal antibody (Product # 40-5300) in Western Blot

Wed May 01 00:00:00 EDT 2019

Caveolin-3 KO disrupts t-tubule structure and decreases t-tubular I_Ca density in mouse ventricular myocytes.

American journal of physiology. Heart and circulatory physiology

Bryant SM,Kong CHT,Watson JJ,Gadeberg HC,Roth DM,Patel HH,Cannell MB,James AF,Orchard CH

40-5300 was used in Western Blotting to study the structure and function of myocytes isolated from Cav-3 knockouts.

Thu Nov 01 00:00:00 EDT 2018

Caveolin 3-dependent loss of t-tubular I_Ca during hypertrophy and heart failure in mice.

Experimental physiology

Bryant SM,Kong CHT,Watson JJ,Gadeberg HC,James AF,Cannell MB,Orchard CH

Published figure using JPH2 polyclonal antibody (Product # 40-5300) in Western Blot

Tue May 01 00:00:00 EDT 2018

Caveolin 3-dependent loss of t-tubular I_Ca during hypertrophy and heart failure in mice.

Experimental physiology

Bryant SM,Kong CHT,Watson JJ,Gadeberg HC,James AF,Cannell MB,Orchard CH

Published figure using JPH2 polyclonal antibody (Product # 40-5300) in Western Blot

Tue May 01 00:00:00 EDT 2018

Deregulated Ca²⁺ cycling underlies the development of arrhythmia and heart disease due to mutant obscurin.

Science advances

Hu LR,Ackermann MA,Hecker PA,Prosser BL,King B,O'Connell KA,Grogan A,Meyer LC,Berndsen CE,Wright NT,Jonathan Lederer W,Kontrogianni-Konstantopoulos A

40-5300 was used in Western Blotting to postulate that enhanced binding between mutant obscurins and phospholamban leads to SERCA2 disinhibition, which may underlie the observed pathological alterations.

Thu Jun 01 00:00:00 EDT 2017

Junctophilin-2 in the nanoscale organisation and functional signalling of ryanodine receptor clusters in cardiomyocytes.

Journal of cell science

Munro ML,Jayasinghe ID,Wang Q,Quick A,Wang W,Baddeley D,Wehrens XH,Soeller C

Published figure using JPH2 polyclonal antibody (Product # 40-5300) in Immunofluorescence

Thu Dec 01 00:00:00 EST 2016

Junctophilin-2 in the nanoscale organisation and functional signalling of ryanodine receptor clusters in cardiomyocytes.

Journal of cell science

Munro ML,Jayasinghe ID,Wang Q,Quick A,Wang W,Baddeley D,Wehrens XH,Soeller C

Published figure using JPH2 polyclonal antibody (Product # 40-5300) in Immunofluorescence

Thu Dec 01 00:00:00 EST 2016

Observation of the molecular organization of calcium release sites in fast- and slow-twitch skeletal muscle with nanoscale imaging.

Journal of the Royal Society, Interface

Jayasinghe ID,Munro M,Baddeley D,Launikonis BS,Soeller C

Published figure using JPH2 polyclonal antibody (Product # 40-5300) in Immunofluorescence

Mon Oct 06 00:00:00 EDT 2014

Ca2+-dependent proteolysis of junctophilin-1 and junctophilin-2 in skeletal and cardiac muscle.

The Journal of physiology

Murphy RM,Dutka TL,Horvath D,Bell JR,Delbridge LM,Lamb GD

40-5300 was used in western blot to study junctophilin proteolysis in skeletal muscle.

Fri Feb 01 00:00:00 EST 2013

Stimulated emission depletion live-cell super-resolution imaging shows proliferative remodeling of T-tubule membrane structures after myocardial infarction.

Circulation research

Wagner E,Lauterbach MA,Kohl T,Westphal V,Williams GS,Steinbrecher JH,Streich JH,Korff B,Tuan HT,Hagen B,Luther S,Hasenfuss G,Parlitz U,Jafri MS,Hell SW,Lederer WJ,Lehnart SE

Published figure using JPH2 polyclonal antibody (Product # 40-5300) in Western Blot

Fri Aug 03 00:00:00 EDT 2012

Stimulated emission depletion live-cell super-resolution imaging shows proliferative remodeling of T-tubule membrane structures after myocardial infarction.

Circulation research

Wagner E,Lauterbach MA,Kohl T,Westphal V,Williams GS,Steinbrecher JH,Streich JH,Korff B,Tuan HT,Hagen B,Luther S,Hasenfuss G,Parlitz U,Jafri MS,Hell SW,Lederer WJ,Lehnart SE

Published figure using JPH2 polyclonal antibody (Product # 40-5300) in Western Blot

Fri Aug 03 00:00:00 EDT 2012

Junctophilin 1 and 2 proteins interact with the L-type Ca2+ channel dihydropyridine receptors (DHPRs) in skeletal muscle.

The Journal of biological chemistry

Golini L,Chouabe C,Berthier C,Cusimano V,Fornaro M,Bonvallet R,Formoso L,Giacomello E,Jacquemond V,Sorrentino V

Fri Dec 23 00:00:00 EST 2011

Junctophilin damage contributes to early strength deficits and EC coupling failure after eccentric contractions.

American journal of physiology. Cell physiology

Corona BT,Balog EM,Doyle JA,Rupp JC,Luke RC,Ingalls CP

40-5300 was used in Western Blotting to indicate that Junctophilin damage plays a role in early force deficits due to excitation-contraction coupling failure following the performance of eccentric contractions.

Mon Feb 01 00:00:00 EST 2010

Bioinformatics

Protein Aliases: JP-2; JP2NT; Junctophilin type 2; Junctophilin-2; Junctophilin-2 N-terminal fragment

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Gene Aliases: 1110002E14Rik; JP-2; Jp2; Jph2

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UniProt ID: (Mouse) Q9ET78

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Entrez Gene ID: (Mouse) 59091

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Function(s)

positive regulation of cytosolic calcium ion concentration multicellular organism development regulation of cardiac muscle tissue development calcium ion homeostasis positive regulation of ryanodine-sensitive calcium-release channel activity calcium ion transmembrane transport

Process(es)

phosphatidylserine binding protein binding phosphatidylinositol-4,5-bisphosphate binding phosphatidylinositol-3,4,5-trisphosphate binding phosphatidylinositol-5-phosphate binding calcium-release channel activity phosphatidylinositol-3-phosphate binding phosphatidylinositol-4-phosphate binding phosphatidic acid binding phosphatidylinositol-3,5-bisphosphate binding

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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JPH2 Polyclonal Antibody

JPH2 Antibody (40-5300) in WB

Product Details

40-5300

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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