Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:1,000-1:4,000 | View 1 publication 1 publication |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:20-1:200 | - |
Immunocytochemistry (ICC/IF) |
1:10-1:100 | - |
Immunoprecipitation (IP) |
0.5-4.0 µg | - |
Product Specifications | |
---|---|
Species Reactivity |
Hamster, Human, Mouse |
Published species |
Mouse |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
JUN Fusion Protein Ag17639 (92-227 aa encoded by BC068522) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.73 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.1% sodium azide |
Storage conditions |
-20°C |
This antibody is a rabbit polyclonal antibody raised against a region of human JUN. Both phosphorylated (p-c-Jun) and unphosphorylated forms of c-Jun, with sizes of 42-45 kDa and 36-39 kDa, respectively are obtain in some experiments.
Immunogen sequence: PTPTQFLCP KNVTDEQEGF AEGFVRALAE LHSQNTLPSV TSAAQPVNGA GMVAPAVASV AGGSGSGGFS ASLHSEPPVY ANLSNFNPGA LSSGGGAPSY GAAGLAFPAQ PQQQQQPPHH LPQQMPVQHP RLQALKE (92-227 aa encoded by BC068522)
c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Activator protein 1; AH119; AP1; cjun; enhancer-binding protein AP1; immediate early; Jun A; Jun activation domain binding protein; Jun oncogene; jun proto-oncogene; JUND; p39; Proto-oncogene c-Jun; Transcription factor AP-1; V-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog
Gene Aliases: AP-1; AP1; c-Jun; I79_009953; JUN; Junc
UniProt ID: (Human) P05412, (Mouse) P05627
Entrez Gene ID: (Human) 3725, (Chinese hamster) 100766306, (Mouse) 16476
Molecular Function:
basic leucine zipper transcription factor
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