Invitrogen

JunB Polyclonal Antibody

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Datasheet

Protocols

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JunB Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence staining of JUNB in HUVEC cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with JunB Polyclonal Antibody (Product # PA5-81561, 1:5,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.

JunB Antibody in Western Blot (WB) — Western Blot using JunB Polyclonal Antibody (Product # PA5-81561) at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 40 kDa. (We are unsure of the identity of these extra bands).

JunB Antibody in Immunoprecipitation (IP) — JunB Immunoprecipitation using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate, Lane B: 0.5 mg Jurkat Whole Cell Lysate 1 µL with JunB Polyclonal Antibody (Product # PA5-81561) and 15 µL of 50 % Protein G agarose. Primary antibody: JunB Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 36 kDa.

Product Details

PA5-81561

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunocytochemistry (ICC/IF)	1:1500-1:50000	-
Immunoprecipitation (IP)	1-2 µL/mg of lysate	-

Product Specifications
Species Reactivity	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthetic peptide corresponding to the N-terminus of the Mouse JunB View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	Lot-Specific
Purification	Protein A, Antigen affinity chromatography
Storage buffer	PBS
Contains	no preservative
Storage conditions	Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
RRID	AB_2788761

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Mouse JunB.

Target Information

JunB, a member of the Jun cellular oncogene family, is a basic region, leucine zipper transcription factor. It is involved in regulating gene activity following the primary growth factor response. JunB binds to the DNA sequence 5'-TGA[CG]TCA-3'. Cellular oncogenes play critical roles in cellular communication pathways that regulate growth, development, and differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Jun B; Jun-B oncogene; MyD21; Oncogene Jun-B; Transcription factor AP-1 subunit JunB; transcription factor jun-B; Transcription factor JunB

Gene Aliases: Jun-b; Junb

UniProt ID: (Mouse) P09450

Entrez Gene ID: (Mouse) 16477

Molecular Function: basic leucine zipper transcription factor