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ATG14 Antibody (710702) in WB
Western blot analysis was performed on whole cell extracts (50 µg lysate) of HeLa serum starved and treated with Rapamycin (100nM/4 hours) (Lane1), HeLa Serum starved (Lane2), HeLa (Lane 3), A549 serum starved treated with Rapamycin (100nM/4 hours) (Lane 4), A549 serum starved (Lane5), A549 (Lane6), U-87-MG serum starved and treated with Rapamycin (100nM/4 hours) (Lane7) and U-87-MG (Lane8). The blots were probed with Anti- ATG 14 Recombinant Rabbit Superclonal™ Antibody (Product # 710702, 1-3 µg/mL) and detected by chemiluminescence using... View More
Product Details
710702
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Immunogen
3 synthetic peptides corresponding to human ATG14 (aa 63-79, 162-178, 277-293)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Rat, Mouse, Pig, Bovine, Rabbit and Dog.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
ATG14 (Autophagy related protein homolog 14) is encoded by the ATG14 gene and is located on chromosome 14 in humans. It is also known as ATG14L or BARKOR (Beclin 1-associated autophagy-related key regulator). Autophagy is an evolutionarily conserved process that involves recycling of misfolded cellular proteins and degradation of dysfunctional organelles. Autophagy is induced as a cellular response to nutrient stress which includes the formation of autophagosomes, fusion of these with the lysosome and formation of the autophagolysosome. The Class III PI3-Kinase, Vps34 and its interacting partner Beclin 1 have been shown to form a complex which specifically includes ATG 14 (type 1) or UVRAG (type 2). Atg 14 guides the type 1 complex to the preautophagosomal structure (PAS) and is critical for the initiation of autophagosome formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATG14 autophagy related 14 homolog; Atg14L; Autophagy-related protein 14-like protein; Bakor; Barkor; Beclin 1-associated autophagy-related key regulator; Beclin 1-Interacting protein; unnamed protein product
Gene Aliases: ATG14; ATG14L; BARKOR; KIAA0831
UniProt ID: (Human) Q6ZNE5
Entrez Gene ID: (Human) 22863
autophagosome assembly
mitophagy
negative regulation of protein phosphorylation
positive regulation of protein phosphorylation
protein targeting to lysosome
autophagy
endosome to lysosome transport
cellular response to starvation
posttranscriptional regulation of gene expression
macroautophagy
autophagosome membrane docking
regulation of macroautophagy
phosphatidylinositol-3-phosphate biosynthetic process
cellular response to glucose starvation
protein kinase B signaling
early endosome to late endosome transport
innate immune response
defense response to virus
regulation of protein complex stability
regulation of triglyceride metabolic process
autophagosome maturation
response to mitochondrial depolarisation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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