Invitrogen

K-Ras Polyclonal Antibody

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1 Published Figure

7 References

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Datasheet

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Questions & Answers

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Datasheet

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Protocols

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Additional Information:

FIGURE: 1 / 4

K-Ras Antibody (PA5-44339) in WB

Western blot analysis of human RPMI-8226 cells using an anti-K-Ras polyclonal antibody (Product # PA5-44339).

K-Ras Antibody in Immunohistochemistry (IHC)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

K-Ras Antibody — Degradation of K-RAS requires autophagosome formation. (a). PANC-1 pancreatic and HCT116 colorectal tumor cells were transfected with plasmids to express K-RAS V12-GFP and K-RAS V12-RFP. In parallel, cells were transfected with a scrambled siRNA control or with siRNA molecules to knock down the expression of ATG5, Beclin1 or LAMP2. Twenty-four h after transfection cells were treated with vehicle control or with neratinib (100 nM). Cells were imaged at 60X magnification over the time course shown in the Figure (representative images from three studies are shown). Analyses for densitometric scanning of fluorescence intensity at 3h were performed at 10X magnification on > 120 cells per condition (n = 3 SD) * p < 0.05 less than vehicle control exposure. (b). PANC-1 and HCT116 cells were transfected with a scrambled control or with siRNA molecules to knock down the expression of K-RAS or N-RAS. Twenty-four h after transfection, cells were fixed in place and immune-staining performed to detect the fluorescence intensity under each condition. Analyses for densitometric scanning of fluorescence intensity at 3h were performed at 10X magnification on > 120 cells per condition (n = 3 SD) * p < 0.05 less than vehicle control exposure. (c). Uveal melanoma cells were transfected with a scrambled siRNA control or with siRNA molecules to knock down the expression of ATG5 or Beclin1. Twenty-four h after transfection cells were treated with vehicle control or with neratinib (100 nM). Cells {KD}

Immunogen image

Immunogen Image K-Ras Polyclonal Antibody

Product Details

PA5-44339

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-1 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide directed towards the N-terminal of human KRAS (aa 2-51).

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

50 µg

Purification

Affinity Chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2609818

Product Specific Information

Peptide sequence: TEYKLVVVGA GGVGKSALTI QLIQNHFVDE YDPTIEDSYR KQVVIDGETC

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 100%; Zebrafish: 100%

Target Information

K-RAS (GTPase KRas) belongs to the Ras oncogene family. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. It plays an important role in the regulation of cell proliferation and has a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes in colorectal cancer cells in a ZNF304-dependent manner. Mutations in the gene can result in leukemia acute myelogenous, leukemia juvenile myelomonocytic, Noonan Syndrome, gastric cancer, and cardiofaciocutaneous syndrome 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: c-K-ras; c-Ki-ras; cellular c-Ki-ras2; GTPase KRas; K RAS; K-Ras 2; Ki-Ras; Kirsten rat sarcoma oncogene 2, expressed; p21 protein; RASK

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Gene Aliases: AI929937; K-ras; Ki-ras; KRAS; Kras-2; KRAS2; p21B; ras; RASK2

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UniProt ID: (Mouse) P32883

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Entrez Gene ID: (Mouse) 16653

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Function(s)

nucleotide binding GTPase activity protein binding GTP binding GMP binding GDP binding LRR domain binding protein complex binding small GTPase

Process(es)

positive regulation of protein phosphorylation signal transduction small GTPase mediated signal transduction Ras protein signal transduction positive regulation of cell proliferation visual learning positive regulation of gene expression cytokine-mediated signaling pathway actin cytoskeleton organization regulation of synaptic transmission, GABAergic positive regulation of Rac protein signal transduction positive regulation of MAP kinase activity negative regulation of neuron apoptotic process negative regulation of cell differentiation regulation of long-term neuronal synaptic plasticity homeostasis of number of cells within a tissue positive regulation of nitric-oxide synthase activity positive regulation of NF-kappaB transcription factor activity striated muscle cell differentiation epithelial tube branching involved in lung morphogenesis

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K-Ras Polyclonal Antibody

K-Ras Antibody (PA5-44339) in WB

Product Details

PA5-44339

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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