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Invitrogen
K-Ras Monoclonal Antibody (A8E4)
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FIGURE: 1 / 4
K-Ras Antibody (MA5-44932) in ICC/IF
Immunocytochemistry analysis of KRAS in HeLa cells. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Samples were incubated in KRAS Monoclonal antibody (Product # MA5-44932) using a dilution of 1:200 in 2% BSA overnight at 4 &(8)451; followed by Goat Anti-Mouse IgG H&L (iFluor™ 488) secondary antibody at a dilution of 1:1,000. PBS instead of the primary antibody was used as the secondary antibody only control.... View More
Product Details
MA5-44932
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
A8E4
Immunogen
Recombinant protein within human KRAS aa 2-186.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
K-RAS (GTPase KRas) belongs to the Ras oncogene family. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. It plays an important role in the regulation of cell proliferation and has a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes in colorectal cancer cells in a ZNF304-dependent manner. Mutations in the gene can result in leukemia acute myelogenous, leukemia juvenile myelomonocytic, Noonan Syndrome, gastric cancer, and cardiofaciocutaneous syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: c-K-ras; c-K-ras2 protein; c-Ki-ras; c-Kirsten-ras protein; c-Kirsten-ras proto-oncogene; c-ras-Ki-2 protein; cellular c-Ki-ras2; cellular c-Ki-ras2 proto-oncogene; cellular transforming proto-oncogene; GTPase KRas; K RAS; K-Ras 2; K-ras p21 protein; Ki-Ras; Kirsten rat sarcoma oncogene 2, expressed; Kirsten rat sarcoma viral oncogene homolog; Kirsten rat sarcoma viral oncogene homologue 2 (active); Kirsten rat sarcoma viral proto-oncogene; KRAS-ENOPH fusion; Kras-Enoph1 fusion protein; oncogene KRAS2; p21 protein; PR310 c-K-ras oncogene; PR371 c-K-ras oncogene; proto-oncogene GTPase; RASK; small GTP binding protein; transforming protein p21; unnamed protein product; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog; v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Gene Aliases: 'C-K-RAS; AI929937; C-K-RAS; c-Ki-ras; c-Ki-ras2; CFC2; K-Ras; K-Ras 2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS; Kras-2; KRAS1; KRAS2; NS; NS3; OES; p21; p21B; RALD; ras; RASK2
UniProt ID: (Human) P01116, (Rat) P08644, (Mouse) P32883
Entrez Gene ID: (Human) 3845, (Rat) 24525, (Mouse) 16653
MAPK cascade
liver development
signal transduction
Ras protein signal transduction
female pregnancy
cell proliferation
visual learning
response to gravity
gene expression
positive regulation of gene expression
glial cell proliferation
Rac protein signal transduction
cytokine-mediated signaling pathway
forebrain astrocyte development
actin cytoskeleton organization
negative regulation of epithelial cell differentiation
regulation of synaptic transmission, GABAergic
positive regulation of Rac protein signal transduction
response to isolation stress
skeletal muscle cell differentiation
negative regulation of neuron apoptotic process
regulation of long-term neuronal synaptic plasticity
homeostasis of number of cells within a tissue
striated muscle cell differentiation
response to glucocorticoid
response to mineralocorticoid
neuron apoptotic process
myoblast proliferation
cardiac muscle cell proliferation
positive regulation of glial cell proliferation
epithelial tube branching involved in lung morphogenesis
Type I pneumocyte differentiation
positive regulation of cellular senescence
positive regulation of protein phosphorylation
small GTPase mediated signal transduction
positive regulation of cell proliferation
social behavior
positive regulation of MAP kinase activity
negative regulation of cell differentiation
positive regulation of nitric-oxide synthase activity
positive regulation of NF-kappaB transcription factor activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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