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Proteintech
KAP1 Polyclonal Antibody, CoraLite® Plus 488
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Product Details
CL48815202100UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Conjugate
Excitation/Emission Max
493/522 nm
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.5% BSA
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Wet ice
Target Information
The KRAB (Kruppel-associated box) domain is about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. There have been identified over 220 KRAB-zinc finger protein (KRAB-ZFP) genes in the human genome. These proteins functionally repress transcription via specific interactions with KAP-1 (KRAB-associated protein 1). KAP-1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. KAP-1 has been shown to form complexes with KRAB-domain transcription factors and increase the efficiency with which they mediate repression. KAP-1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1). KAP-1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, thus helping to regulate transcriptional repression. Studies have shown that KAP-1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 SUMO-protein ligase TRIM28; KAP-1; KRAB [Kruppel-associated box domain]-associated protein 1; KRAB-associated protein 1; KRAB-interacting p; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; protein phosphatase 1, regulatory subunit 157; RING finger protein 96; RING-type E3 ubiquitin transferase TIF1-beta; tif1 beta; TIF1-beta; Transcription intermediary factor 1-beta; transcriptional intermediary factor 1-beta; tripartite motif; Tripartite motif-containing protein 28
Gene Aliases: KAP1; PPP1R157; RNF96; TF1B; TIF1B; TRIM28
UniProt ID: (Human) Q13263
Entrez Gene ID: (Human) 10155
RNA polymerase II transcription coactivator activity
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
protein kinase activity
ubiquitin-protein transferase activity
protein binding
zinc ion binding
ligase activity
ubiquitin protein ligase binding
Krueppel-associated box domain binding
sequence-specific DNA binding
poly(A) RNA binding
chromo shadow domain binding
promoter-specific chromatin binding
negative regulation of transcription from RNA polymerase II promoter
epithelial to mesenchymal transition
DNA repair
transcription initiation from RNA polymerase II promoter
Ras protein signal transduction
embryo implantation
protein ubiquitination
covalent chromatin modification
protein sumoylation
positive regulation of transcription factor import into nucleus
DNA methylation involved in embryo development
innate immune response
positive regulation of DNA repair
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
protein autophosphorylation
protein oligomerization
convergent extension involved in axis elongation
embryonic placenta morphogenesis
positive regulation of methylation-dependent chromatin silencing
negative regulation of DNA demethylation
negative regulation of viral release from host cell
regulation of genetic imprinting
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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