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Invitrogen
KAP3 Polyclonal Antibody
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FIGURE: 1 / 5
KAP3 Antibody (PA5-54878) in ICC/IF
Immunofluorescence analysis of KAP3 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with KAP3 Polyclonal Antibody (Product # PA5-54878) at 5µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a di... View More
Product Details
PA5-54878
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human KAP3. Recombinant protein control fragment (Product #RP-94021).
Conjugate
Form
Liquid
Concentration
0.05 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: ELLNAQQEDD EFVCQIIYVF YQMVFHQATR DVIIKETQAP AYLIDLMHDK NNEIRKVCDN TLDIIAEYDE EWAKK
Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.
Target Information
The small G protein GDP dissociation stimulator (smg GDS) is a regulator protein having two activities on a group of small G proteins including the Rho and Rap1 family members and Ki-Ras; one is to stimulate their GDP/GTP exchange reactions, and the other is to inhibit their interactions with membranes. The protein encoded by this gene contains 9 'Armadillo' repeats and interacts with the smg GDS protein through these repeats. This protein, which is highly concentrated around the endoplasmic reticulum, is phosphorylated by v-src, and this phosphorylation reduces the affinity of the protein for smg GDS. It is thought that this protein serves as a linker between human chromosome-associated polypeptide (HCAP) and KIF3A/B, a kinesin superfamily protein in the nucleus, and that it plays a role in the interaction of chromosomes with an ATPase motor protein. Several transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: KAP-3; Kinesin-associated protein 3; small G protein GDP dissociation stimulator; Smg GDS-associated protein
Gene Aliases: dJ190I16.1; FLA3; KAP-1; KAP-3; KAP3; KIF3AP; KIFAP3; SMAP; Smg-GDS
UniProt ID: (Human) Q92845
Entrez Gene ID: (Human) 22920
protein complex assembly
retrograde vesicle-mediated transport, Golgi to ER
microtubule-based process
microtubule-based movement
signal transduction
protein localization
negative regulation of cell proliferation
antigen processing and presentation of exogenous peptide antigen via MHC class II
negative regulation of apoptotic process
positive regulation of calcium-dependent cell-cell adhesion
plus-end-directed vesicle transport along microtubule
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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