Proteintech

KAT5 Polyclonal Antibody

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KAT5 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 10% Formaldehyde ) fixed PC-3 cells using 10827-1-AP (KAT5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

KAT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate cancer using 10827-1-AP (KAT5 antibody) at dilution of 1:100 (under 10x lens).

KAT5 Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 10827-1-AP (KAT5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Product Details

10827-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

ChIP assay (ChIP)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Not applicable (N/A)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KAT5 Fusion Protein Ag1261 (101-457 aa encoded by BC000166)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.43 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: ATPVPSETA PASVFPQNGA ARRAVAAQPG RKRKSNCLGT DEDSQDSSDG IPSAPRMTGS LVSDRSHDDI VTRMKNIECI ELGRHRLKPW YFSPYPQELT TLPVLYLCEF CLKYGRSLKC LQRHLTKCDL RHPPGNEIYR KGTISFFEID GRKNKSYSQN LCLLAKCFLD HKTLYYDTDP FLFYVMTEYD CKGFHIVGYF SKEKESTEDY NVACILTLPP YQRRGYGKLL IEFSYELSKV EGKTGTPEKP LSDLGLLSYR SYWSQTILEI LMGLKSESGE RPQITINEIS EITSIKKEDV ISTLQYLNLI NYYKGQYILT LSEDIVDGHE RAMLKRLLRI DSKCLHFTPK DWSKRGKW (101-457 aa encoded by BC000166)

Target Information

TIP60 is a transcription factor, originally isolated as an HIV-1 TAT-interactive protein, known to interact with the viral TAT protein that leads to HTATIP polyubiquitination and targets it for degradation. It belongs to the MYST family of histone acetyl transferases (HATs). TIP60 is a catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. A co-regulator of transcription factors, TIP60 either promotes or suppresses tumorigenesis. TIP60 is an enzyme that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ULK1-mediated metabolic reprogramming regulates Vps34 lipid kinase activity by its lactylation.

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Jia M,Yue X,Sun W,Zhou Q,Chang C,Gong W,Feng J,Li X,Zhan R,Mo K,Zhang L,Qian Y,Sun Y,Wang A,Zou Y,Chen W,Li Y,Huang L,Yang Y,Zhao Y,Cheng X

Fri Jun 02 00:00:00 EDT 2023

A NPAS4-NuA4 complex couples synaptic activity to DNA repair.

Nature

Pollina EA,Gilliam DT,Landau AT,Lin C,Pajarillo N,Davis CP,Harmin DA,Yap EL,Vogel IR,Griffith EC,Nagy MA,Ling E,Duffy EE,Sabatini BL,Weitz CJ,Greenberg ME

Wed Feb 01 00:00:00 EST 2023

Dual-specificity RNA aptamers enable manipulation of target-specific O-GlcNAcylation and unveil functions of O-GlcNAc on β-catenin.

Cell

Zhu Y,Hart GW

Thu Jan 19 00:00:00 EST 2023

Acetylation of Nup62 by TIP60 ensures accurate chromosome segregation in mitosis.

Journal of molecular cell biology

Akbar H,Cao J,Wang D,Yuan X,Zhang M,Muthusamy S,Song X,Liu X,Aikhionbare F,Yao X,Gao X,Liu X

Mon Dec 26 00:00:00 EST 2022

Acetylation of Nup62 by TIP60 ensures accurate chromosome segregation in mitosis.

Journal of molecular cell biology

Akbar H,Cao J,Wang D,Yuan X,Zhang M,Muthusamy S,Song X,Liu X,Aikhionbare F,Yao X,Gao X,Liu X

Mon Dec 26 00:00:00 EST 2022

Acquired temozolomide resistance in MGMT^low gliomas is associated with regulation of homologous recombination repair by ROCK2.

Cell death & disease

Zhang X,Li T,Yang M,Du Q,Wang R,Fu B,Tan Y,Cao M,Chen Y,Wang Q,Hu R

Thu Feb 10 00:00:00 EST 2022

Acquired temozolomide resistance in MGMT^low gliomas is associated with regulation of homologous recombination repair by ROCK2.

Cell death & disease

Zhang X,Li T,Yang M,Du Q,Wang R,Fu B,Tan Y,Cao M,Chen Y,Wang Q,Hu R

Thu Feb 10 00:00:00 EST 2022

DNA repair factor KAT5 prevents ischemic acute kidney injury through glomerular filtration regulation.

iScience

Hishikawa A,Hayashi K,Kubo A,Miyashita K,Hashiguchi A,Kinouchi K,Yoshimoto N,Nakamichi R,Akashio R,Sugita E,Azegami T,Monkawa T,Suematsu M,Itoh H

Fri Dec 17 00:00:00 EST 2021

Acetylated DNMT1 Downregulation and Related Regulatory Factors Influence Metastatic Melanoma Patients Survival.

Cancers

Zhang X,Bustos MA,Shoji Y,Ramos RI,Iida Y,Gentry R,Takeshima TL,Hoon DSB

Sat Sep 18 00:00:00 EDT 2021

Impaired FGF10 Signaling and Epithelial Development in Experimental Lung Hypoplasia With Esophageal Atresia.

Frontiers in pediatrics

Wang J,Liu H,Gao L,Liu X

Wed Sep 30 00:00:00 EDT 2020

Impaired FGF10 Signaling and Epithelial Development in Experimental Lung Hypoplasia With Esophageal Atresia.

Frontiers in pediatrics

Wang J,Liu H,Gao L,Liu X

Wed Sep 30 00:00:00 EDT 2020

Posttranslational lysine 2-hydroxyisobutyrylation of human sperm tail proteins affects motility.

Human reproduction (Oxford, England)

Cheng YM,Peng Z,Chen HY,Pan TT,Hu XN,Wang F,Luo T

Fri Mar 27 00:00:00 EDT 2020

Requirement for p62 acetylation in the aggregation of ubiquitylated proteins under nutrient stress.

Nature communications

You Z,Jiang WX,Qin LY,Gong Z,Wan W,Li J,Wang Y,Zhang H,Peng C,Zhou T,Tang C,Liu W

Thu Dec 19 00:00:00 EST 2019

Tip60 Suppresses Cholangiocarcinoma Proliferation and Metastasis via PI3k-AKT.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Zhang Y,Ji G,Han S,Shao Z,Lu Z,Huo L,Zhang J,Yang R,Feng Q,Shen H,Wang H,Li X

Fri Nov 02 00:00:00 EDT 2018

Tip60 Suppresses Cholangiocarcinoma Proliferation and Metastasis via PI3k-AKT.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Zhang Y,Ji G,Han S,Shao Z,Lu Z,Huo L,Zhang J,Yang R,Feng Q,Shen H,Wang H,Li X

Fri Nov 02 00:00:00 EDT 2018

The transcriptional coactivator TAZ regulates reciprocal differentiation of T_H17 cells and T_reg cells.

Nature immunology

Geng J,Yu S,Zhao H,Sun X,Li X,Wang P,Xiong X,Hong L,Xie C,Gao J,Shi Y,Peng J,Johnson RL,Xiao N,Lu L,Han J,Zhou D,Chen L

Sat Jul 01 00:00:00 EDT 2017

The transcriptional coactivator TAZ regulates reciprocal differentiation of T_H17 cells and T_reg cells.

Nature immunology

Geng J,Yu S,Zhao H,Sun X,Li X,Wang P,Xiong X,Hong L,Xie C,Gao J,Shi Y,Peng J,Johnson RL,Xiao N,Lu L,Han J,Zhou D,Chen L

Sat Jul 01 00:00:00 EDT 2017

Histone Variant H2A.L.2 Guides Transition Protein-Dependent Protamine Assembly in Male Germ Cells.

Molecular cell

Barral S,Morozumi Y,Tanaka H,Montellier E,Govin J,de Dieuleveult M,Charbonnier G,Couté Y,Puthier D,Buchou T,Boussouar F,Urahama T,Fenaille F,Curtet S,Héry P,Fernandez-Nunez N,Shiota H,Gérard M,Rousseaux S,Kurumizaka H,Khochbin S

Thu Apr 06 00:00:00 EDT 2017

Histone Variant H2A.L.2 Guides Transition Protein-Dependent Protamine Assembly in Male Germ Cells.

Molecular cell

Thu Apr 06 00:00:00 EDT 2017

MDR1 mediated chemoresistance: BMI1 and TIP60 in action.

Biochimica et biophysica acta

Banerjee Mustafi S,Chakraborty PK,Naz S,Dwivedi SK,Street M,Basak R,Yang D,Ding K,Mukherjee P,Bhattacharya R

Mon Aug 01 00:00:00 EDT 2016

MDR1 mediated chemoresistance: BMI1 and TIP60 in action.

Biochimica et biophysica acta

Banerjee Mustafi S,Chakraborty PK,Naz S,Dwivedi SK,Street M,Basak R,Yang D,Ding K,Mukherjee P,Bhattacharya R

Mon Aug 01 00:00:00 EDT 2016

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Molecular & cellular proteomics : MCP

Yu H,Diao H,Wang C,Lin Y,Yu F,Lu H,Xu W,Li Z,Shi H,Zhao S,Zhou Y,Zhang Y

Wed Apr 01 00:00:00 EDT 2015

Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm).

Molecular & cellular proteomics : MCP

Yu H,Diao H,Wang C,Lin Y,Yu F,Lu H,Xu W,Li Z,Shi H,Zhao S,Zhou Y,Zhang Y

Wed Apr 01 00:00:00 EDT 2015

ANP32E is a histone chaperone that removes H2A.Z from chromatin.

Nature

Obri A,Ouararhni K,Papin C,Diebold ML,Padmanabhan K,Marek M,Stoll I,Roy L,Reilly PT,Mak TW,Dimitrov S,Romier C,Hamiche A

Thu Jan 30 00:00:00 EST 2014

ANP32E is a histone chaperone that removes H2A.Z from chromatin.

Nature

Obri A,Ouararhni K,Papin C,Diebold ML,Padmanabhan K,Marek M,Stoll I,Roy L,Reilly PT,Mak TW,Dimitrov S,Romier C,Hamiche A

Thu Jan 30 00:00:00 EST 2014

Bioinformatics

Protein Aliases: 60 kDa Tat-interactive protein; cPLA(2)-interacting protein; cPLA2 interacting protein; Histone acetyltransferase HTATIP; Histone acetyltransferase KAT5; HIV-1 Tat interactive protein; HIV-1 tat interactive protein 1, 60 kDa homolog; HIV-1 tat interactive protein 1, homolog; HIV-1 Tat interactive protein 60 kD; HIV-1 Tat interactive protein, 60 kD; HIV-1 Tat interactive protein, 60kDa; HIV-1 tat interactive protein, homolog; K(lysine) acetyltransferase 5; K-acetyltransferase 5; Lysine acetyltransferase 5; Protein 2-hydroxyisobutyryltransferase KAT5; Protein acetyltransferase KAT5; Protein crotonyltransferase KAT5; Tat interacting protein, 60kDa; Tat-interactive protein-60; Tip60; TIP60(HTATIP)

Gene Aliases: AI839539; CPLA2; ESA1; HTATIP; HTATIP1; KAT5; PLIP; TIP; Tip55; TIP60; Tip60b; ZC2HC5

UniProt ID: (Human) Q92993, (Mouse) Q8CHK4, (Rat) Q99MK2

Entrez Gene ID: (Human) 10524, (Mouse) 81601, (Rat) 192218

Molecular Function: histone modifying enzyme