Osenses

KCNH2 Polyclonal Antibody

View all (14) KCNH2 antibodies

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Datasheet

Protocols

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KCNH2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — IHC-P on paraffin sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 mL 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1:3000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

KCNH2 Antibody in Western Blot (WB) — WB on mouse tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated at 4°C overnight.

Product Details

OSP00150W-100UL

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:3,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / Ig
Class	Polyclonal
Type	Antibody
Immunogen	A synthetic peptide from aa region 1120-1162 of mouse KCNH2 conjugated to blue carrier protein was used as the antigen View immunogen
Conjugate	Unconjugated
Form	Lyophilized
Storage buffer	whole serum
Contains	no preservative
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Product Specific Information

Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.

The peptide is homologous in rat and human.

Specificity of this antibody: KCNS2.

Target Information

Human ether-a-go-go related gene (HERG) encodes the pore-forming alpha subunit of the delayed rectifier potassium channel IKr. There are two N-terminal splice variants of HERG include the full-length isoform 1 alpha and the shorter isoform 1 beta. Isoform 1 beta lacks the PAS motif and deactivates at a faster rate than isoform 1alpha. Residues within the C-terminal play a role in channel expression and channel gating, including voltage-dependent activation. HERG is expressed in the heart and is more abundantly expressed in the ventricles than in the atria. Mutations in the gene encoding HERG increase beat-to-beat variability and early after depolarization. These fluctuations facilitate the genesis and propagation of premature heartbeats associated with inheritable long QT syndrome type 2 and short QT syndrome type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Eag homolog; eag-related protein 1; ERG-1; Ether-a-go-go-related gene potassium channel 1; ether-a-go-go-related potassium channel protein; ether-a-go-go-related protein 1; HERG-1; M-erg1; potassium channel, voltage gated eag related subfamily H, member 2; Potassium voltage-gated channel subfamily H member 2; potassium voltage-gated channel, subfamily H (eag-related), member 2; r-ERG; RERG; Voltage-gated potassium channel subunit Kv11.1

Gene Aliases: AI326795; ERG; ERG-1; ERG1; H-ERG; HERG; HERG1; KCNH2; Kv11.1; LQT; LQT2; M-erg; Merg1; merg1a; merg1b; SQT1

UniProt ID: (Human) Q12809, (Mouse) O35219, (Rat) O08962

Entrez Gene ID: (Human) 3757, (Mouse) 16511, (Rat) 117018

Molecular Function: ion channel