Invitrogen

KCNH2 Polyclonal Antibody

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KCNH2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence staining of HERG in HEK 293 cells using Product # PA3-860.

KCNH2 Antibody in Western Blot (WB) — Published figure using KCNH2 polyclonal antibody (Product # PA3-860) in Western Blot

Product Details

PA3-860

Applications	Tested Dilution	Publications
Western Blot (WB)	1:20,000	View 2 publications 2 publications
Immunocytochemistry (ICC/IF)	1:3000	-
Immunoprecipitation (IP)	1:100	-

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Fusion Protein of HERG encoding 181 amino acid residues from the C-terminus. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	whole serum
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2132141

Product Specific Information

PA3-860 detects HERG in human samples.

PA3-860 has been used successfully in Western blot, immunfluorescence, and immunoprecipitation procedures. By Western blot this antibody detects the ~135 kDa and ~155 kDa forms of HERG.

The PA3-860 immunogen corresponds to a cDNA fragment of HERG encoding 181 amino acid residues from the C-terminus.

Target Information

Human ether-a-go-go related gene (HERG) encodes the pore-forming alpha subunit of the delayed rectifier potassium channel IKr. There are two N-terminal splice variants of HERG include the full-length isoform 1 alpha and the shorter isoform 1 beta. Isoform 1 beta lacks the PAS motif and deactivates at a faster rate than isoform 1alpha. Residues within the C-terminal play a role in channel expression and channel gating, including voltage-dependent activation. HERG is expressed in the heart and is more abundantly expressed in the ventricles than in the atria. Mutations in the gene encoding HERG increase beat-to-beat variability and early after depolarization. These fluctuations facilitate the genesis and propagation of premature heartbeats associated with inheritable long QT syndrome type 2 and short QT syndrome type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ZC88, a novel 4-amino piperidine analog, inhibits the growth of neuroblastoma cells through blocking hERG potassium channel.

Cancer biology & therapy

Wei X,Sun H,Yan H,Zhang C,Zhang S,Liu X,Hua N,Ma X,Zheng J

Published figure using KCNH2 polyclonal antibody (Product # PA3-860) in Western Blot

Wed May 01 00:00:00 EDT 2013

Chronic inhibition of cardiac Kir2.1 and HERG potassium channels by celastrol with dual effects on both ion conductivity and protein trafficking.

The Journal of biological chemistry

Sun H,Liu X,Xiong Q,Shikano S,Li M

PA3-860 was used in western blot to study the effect of celastrol on cardiac Kir2.1 and hERG potassium channels.

Fri Mar 03 00:00:00 EST 2006

Bioinformatics

Protein Aliases: Eag homolog; eag-related protein 1; ERG-1; Ether-a-go-go-related gene potassium channel 1; ether-a-go-go-related potassium channel protein; ether-a-go-go-related protein 1; HERG-1; M-erg1; potassium channel, voltage gated eag related subfamily H, member 2; Potassium voltage-gated channel subfamily H member 2; potassium voltage-gated channel, subfamily H (eag-related), member 2; Voltage-gated potassium channel subunit Kv11.1

Gene Aliases: ERG; ERG-1; ERG1; H-ERG; HERG; HERG1; KCNH2; Kv11.1; LQT2; SQT1

UniProt ID: (Human) Q12809

Entrez Gene ID: (Human) 3757

Molecular Function: ion channel

Function(s)

phosphorelay signal transduction system regulation of heart rate by hormone signal transduction by protein phosphorylation cellular response to drug regulation of membrane potential potassium ion homeostasis cardiac muscle contraction regulation of membrane repolarization regulation of ventricular cardiac muscle cell membrane repolarization cardiac conduction potassium ion export potassium ion transmembrane transport ventricular cardiac muscle cell action potential membrane depolarization during action potential membrane repolarization during action potential membrane repolarization during cardiac muscle cell action potential regulation of heart rate by cardiac conduction potassium ion export across plasma membrane membrane repolarization during ventricular cardiac muscle cell action potential regulation of potassium ion transmembrane transport negative regulation of potassium ion transmembrane transport positive regulation of potassium ion transmembrane transport negative regulation of potassium ion export

Process(es)

phosphorelay sensor kinase activity inward rectifier potassium channel activity voltage-gated potassium channel activity delayed rectifier potassium channel activity protein binding ubiquitin protein ligase binding identical protein binding protein homodimerization activity C3HC4-type RING finger domain binding voltage-gated potassium channel activity involved in cardiac muscle cell action potential repolarization scaffold protein binding voltage-gated potassium channel activity involved in ventricular cardiac muscle cell action potential repolarization

KCNH2 Polyclonal Antibody

Product Details

PA3-860

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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