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Invitrogen
KCNMA1 Polyclonal Antibody
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FIGURE: 1 / 1
KCNMA1 Antibody (PA1-923) in ICC/IF
eLife 2013 -
Figure 10. Slo1 protein is present in human spermatozoa. ( A ) Human sperm immunostaining with primary polyclonal anti-Slo1 antibodies and Cy3-conjugated secondary antibodies. Left and middle panels show Slo1 staining localized to the principal piece of human sperm flagellum. Left panel: nuclei are stained by DAPI. Right panel: DIC image of the same cells. Scale bar is 5 mm. ( B ) Representative immunoblot ... View More
Product Details
PA1-923
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues C T(945) E L V N D T N V Q F L D Q D D D(961).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-923 detects large conductance, calcium-activated potassium channel alpha (Maxi K+ alpha) from human tissues as well as recombinant human protein and rat samples.
PA1-923 has been successfully used in Western blot and immunocytochemistry procedures. By Western blot, this antibody detects an ~110 kDa protein representing Maxi K+ alpha from human myometrial smooth muscle cell extracts. Immunocytochemical staining of Maxi K+ alpha in human coronary muscle cells with PA1-923 results in predominant staining of the plasma membrane.
PA1-923 immunizing peptide corresponds to amino acid residues 945-961 from human Maxi K+ alpha. This sequence is completely conserved across many species. PA1-923 immunizing peptide (Cat. # PEP-113) is available for use in neutralization and control experiments.
Target Information
Potassium channels are a group of ubiquitously expressed proteins that serve numerous functions in excitable and non-excitable cells. One class of integral membrane potassium channels is the large conductance, calcium-activated potassium channel (Maxi K+). Maxi K+ differs from most other potassium channels in that its activation is controlled by both increases in intracellular calcium and by membrane depolarization. Maxi K+ dual activation is possible because of its structure. The core of the channel, which is similar to other potassium channels, is a Maxi K+ alpha homotetramer that contains both a voltage sensor and an intracellular calcium binding domain. In vascular smooth muscle, an auxiliary beta-subunit is found in a 1:1 stoichiometry. The beta-subunit exhibits its effect on the Maxi K+ channel by effectively decreasing by 5- to 10- fold the concentration of calcium required to keep the pore open.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: big potassium channel alpha subunit; BK channel; BK channel alpha subunit; BKCA alpha; BKCA alpha subunit; calcium-activated potassium channel alpha subunit; Calcium-activated potassium channel subunit alpha-1; Calcium-activated potassium channel, subfamily M subunit alpha-1; K(VCA)alpha; KCa1.1; Maxi K channel; maxi K channel; KCNMA1; maxi-K channel HSLO; MaxiK; MaxiK alpha subunit; potassium channel; potassium channel, calcium activated large conductance subfamily M alpha, member 1; potassium large conductance calcium-activated channel, subfamily M, alpha member 1; Slo homolog; Slo-alpha; Slo1; Slowpoke homolog; stretch-activated Kca channel; voltage-sensitive calcium channel
Gene Aliases: bA205K10.1; BKCa; BKTM; CADEDS; hSlo; IEG16; KCa1.1; KCNMA; KCNMA1; KCNMA1b; KCNMA1c; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA; SLO1
UniProt ID: (Human) Q12791, (Rat) Q62976
Entrez Gene ID: (Human) 3778, (Rat) 83731
actin binding
ion channel activity
voltage-gated potassium channel activity
potassium channel activity
protein binding
calcium-activated potassium channel activity
identical protein binding
metal ion binding
large conductance calcium-activated potassium channel activity
protein complex binding
protein homodimerization activity
ion channel
response to hypoxia
ion transport
potassium ion transport
response to osmotic stress
cellular potassium ion homeostasis
ion transmembrane transport
response to carbon monoxide
vasodilation
regulation of membrane potential
positive regulation of apoptotic process
negative regulation of cell volume
response to calcium ion
transmembrane transport
micturition
smooth muscle contraction involved in micturition
potassium ion transmembrane transport
chemical synaptic transmission
sensory perception of sound
circadian rhythm
adult walking behavior
response to pH
neuronal action potential
response to corticosteroid
regulation of aldosterone metabolic process
regulation of ion transmembrane transport
regulation of vasodilation
auditory receptor cell differentiation
response to estrogen
locomotor rhythm
saliva secretion
cell maturation
neuromuscular process controlling balance
protein homooligomerization
protein homotetramerization
eye blink reflex
relaxation of vascular smooth muscle
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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