PA1-923 detects large conductance, calcium-activated potassium channel alpha (Maxi K+ alpha) from human tissues as well as recombinant human protein and rat samples.
PA1-923 has been successfully used in Western blot and immunocytochemistry procedures. By Western blot, this antibody detects an ~110 kDa protein representing Maxi K+ alpha from human myometrial smooth muscle cell extracts. Immunocytochemical staining of Maxi K+ alpha in human coronary muscle cells with PA1-923 results in predominant staining of the plasma membrane.
PA1-923 immunizing peptide corresponds to amino acid residues 945-961 from human Maxi K+ alpha. This sequence is completely conserved across many species. PA1-923 immunizing peptide (Cat. # PEP-113) is available for use in neutralization and control experiments.
Potassium channels are a group of ubiquitously expressed proteins that serve numerous functions in excitable and non-excitable cells. One class of integral membrane potassium channels is the large conductance, calcium-activated potassium channel (Maxi K+). Maxi K+ differs from most other potassium channels in that its activation is controlled by both increases in intracellular calcium and by membrane depolarization. Maxi K+ dual activation is possible because of its structure. The core of the channel, which is similar to other potassium channels, is a Maxi K+ alpha homotetramer that contains both a voltage sensor and an intracellular calcium binding domain. In vascular smooth muscle, an auxiliary beta-subunit is found in a 1:1 stoichiometry. The beta-subunit exhibits its effect on the Maxi K+ channel by effectively decreasing by 5- to 10- fold the concentration of calcium required to keep the pore open.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: big potassium channel alpha subunit; BK channel; BK channel alpha subunit; BKCA alpha; BKCA alpha subunit; calcium-activated potassium channel alpha subunit; Calcium-activated potassium channel subunit alpha-1; Calcium-activated potassium channel, subfamily M subunit alpha-1; K(VCA)alpha; KCa1.1; Kcnma; KCNMA1b; KCNMA1c; Maxi K channel; maxi-K channel HSLO; MaxiK; potassium channel, calcium activated large conductance subfamily M alpha, member 1; potassium large conductance calcium-activated channel, subfamily M, alpha member 1; Slo; Slo homolog; Slo-alpha; Slo1; Slowpoke homolog; stretch-activated Kca channel
Gene Aliases: bA205K10.1; BKCa; BKTM; hSlo; KCa1.1; KCNMA; KCNMA1; KCNMA1b; KCNMA1c; MaxiK; mSLO1; SAKCA; SLO; SLO-ALPHA; SLO1