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Invitrogen
KCNMB1 Polyclonal Antibody
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FIGURE: 1 / 10
KCNMB1 Antibody (PA1-924) in ICC/IF
Immunofluorescent analysis of Maxi K+ Beta (green) in Movas cells transfected with (+) and without (-) Maxi K+ Beta siRNA. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 10 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a p38 polyclonal antibody (Product # AHO1202) at a dilution of 1:100 in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (Heavy Chain) Superclona... View More
Product Details
PA1-924
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Baboon,
C. elegans,
Dog,
Human,
Mouse,
Rabbit,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: L(90) Y H T E D T R D Q N Q Q C(103)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-924 detects large conductance, calcium-activated potassium channel beta (Maxi K+ Beta) from human, mouse, and rat samples.
PA1-924 has been successfully used in western blot and immunofluorescence. By western blot, this antibody detects an ~28kDa protein representing Maxi K+ beta from mouse myometrial smooth muscle cell extracts. The molecular weight detected varies due to glycosylation, multimerization and binding to actin.
Immunizing peptide corresponds to amino acid residues 90-103 from human Maxi K+ beta. This sequence is completely conserved across many species. PA1-924 immunizing peptide (Cat. # PEP-119) is available for use in neutralization and control experiments.
Target Information
Potassium channels are a group of ubiquitously expressed proteins that serve numerous functions in excitable and non-excitable cells. One class of integral membrane potassium channels is the large conductance, calcium-activated potassium channel (Maxi K+). Maxi K+ differs from most other potassium channels in that its activation is controlled by both increases in intracellular calcium and by membrane depolarization. Maxi K+ dual activation is possible because of its structure. The core of the channel, which is similar to other potassium channels, is a Maxi K+ alpha homotetramer that contains both a voltage sensor and an intracellular calcium binding domain. In vascular smooth muscle, an auxiliary beta-subunit is found in a 1:1 stoichiometry. The beta-subunit exhibits its effect on the Maxi K+ channel by effectively decreasing by 5- to 10- fold the concentration of calcium required to keep the pore open. Maxi K+ beta is the target for possible therapeutics because of its role in blood flow and blood pressure regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: big potassium channel beta subunit 1; BK channel beta subunit; BK channel beta subunit 1; BK channel subunit beta-1; BKbeta; calcium-activated potassium channel beta subunit; Calcium-activated potassium channel subunit beta; Calcium-activated potassium channel subunit beta-1; Calcium-activated potassium channel, subfamily M subunit beta-1; Charybdotoxin receptor subunit beta-1; K(VCA)beta-1; large conductance Ca2+-activated K+ channel beta 1 subunit; Maxi K channel subunit beta-1; MaxiK channel beta-subunit 1; potassium channel subfamily M regulatory beta subunit 1; potassium large conductance calcium-activated channel, subfamily M, beta member 1; Slo-beta; Slo-beta-1; Slowpoke-beta
Gene Aliases: BKbeta; BKbeta1; hbeta1; hslo-beta; K(VCA)beta; k(VCA)beta-1; KCNMB1; SLO-BETA; slo-beta-1
UniProt ID: (Human) Q16558, (Rat) P97678, (Mouse) Q8CAE3
Entrez Gene ID: (Human) 3779, (Rat) 29747, (Mouse) 16533
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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