|Tested species reactivity||Human, Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 191 of Human KCNMB1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-28284 targets KCNMB1 in WB applications and shows reactivity with Human and mouse samples.
The PA5-28284 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 191 of Human KCNMB1.
MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the product of this gene, the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Western blot analysis of BK channel ß1-subunit expression should be interpreted cautiously when using commercially available antibodies.
PA5-28284 was used in western blot to assess the specificity and sensitivity of six commercially available antibodies to BK beta1 using tissues from wild type and KO mice
|Bhattarai Y,Fernandes R,Kadrofske MM,Lockwood LR,Galligan JJ,Xu H||Physiological reports (2:null)||2014|