Immunofluorescent analysis of KCNQ2 using Anti-KCNQ2 Polyclonal Antibody (Product# PA1-929) shows staining in HepG2 Cells. KCNQ2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing KCNQ2 (Product# PA1-929) at a dilution of 1:100 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552, Goat Anti-Rabbit). Images were taken at 60X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GST fusion protein encoding the first 70 amino acids of human KCNQ2.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||0.5 µg/ml|
|Immunofluorescence (IF)||0.5 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||0.5 µg/ml|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
PA1-929 detects KCNQ2 from mouse, human and rat samples. This antibody is specific for KCNQ2 and does not detect KCNQ1, KCNQ3, KCNQ4 or KCNQ5.
PA1-929 has been successfully used in Western blot, immunofluorescence, immunohistochemistry and immunocytochemistry procedures. Immunohistochemical staining of rat hippocampal using PA1-929 yielded a strong signal in granule cell layer and the mossy fibers found in the central hilus of the dentate gyrus.
PA1-929 immunogen is a GST fusion protein encoding the first 70 amino acids of human KCNQ2.
KCNQs are members of the voltage-dependent non-inactivating potassium channel family. Currently there are five known KNCQs (KCNQ1-5) found in the central nervous system and KNCQ2 and 3 have demonstrated their importance in M-current activation.
Studies have shown that KCNQ2 and KCNQ3 form heteromultimers that, when formed, substantially increase the M-current. Inhibition of M-current controls neuron excitability throughout the nervous system as well as the responsiveness to synaptic inputs. Genetic mutations in these proteins have been linked to disorders such as benign familial neonatal convulsions (BFNC), deafness, neuropathic pain and epilepsy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Giant ankyrin-G: a critical innovation in vertebrate evolution of fast and integrated neuronal signaling.
PA1-929 was used in immunohistochemistry - paraffin section to study the function of the giant exon of ankyrin-G.
|Jenkins PM,Kim N,Jones SL,Tseng WC,Svitkina TM,Yin HH,Bennett V||Proceedings of the National Academy of Sciences of the United States of America (112:957)||2015|