|Tested species reactivity||Human, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C(880) K A G E S T D A L S L P H V K L K(897) of human KCNQ5 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:250|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA1-941 detects the KCNQ5 from rat and human samples. This antibody is specific for KCNQ5 and does not detect KCNQ1, KCNQ2, KCNQ3 or KCNQ4.
PA1-941 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~125 kDa band representing KCNQ5 from rat brain extract.
The PA1-941 immunizing peptide corresponds to amino acid residues 880-897 from human KCNQ5.
KCNQs are members of the voltage-dependent non-inactivating potassium channel family. Currently there are five known KNCQs (KCNQ1-5) found in the central nervous system. Studies have shown that KCNQ3 and KCNQ5 form heteromultimers that, when formed, substantially increase the M-current. Inhibition of M-current controls neuron excitability throughout the nervous system as well as the responsiveness to synaptic inputs. Genetic mutations in these proteins have been linked to disorders such as benign familial neonatal convulsions (BFNC), deafness, neuropathic pain and epilepsy.
Voltage-dependent potassium channels are key regulators of the resting membrane potential and modulate the excitability of electrically active cells. The channels are usually tetrameric and can interact with auxiliary subunits that enhance or modify currents mediated by the pore-forming subunits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The KCNQ/M-current modulates arterial baroreceptor function at the sensory terminal in rats.
PA1-941 was used in immunohistochemistry to investigate the role of KCNQ/M-current on arterial baroreceptor function at the sensory terminal in rats
|Wladyka CL,Feng B,Glazebrook PA,Schild JH,Kunze DL||The Journal of physiology (586:795)||2008|