KChIP3 Polyclonal Antibody

Product Specific Information

PA1-928 detects Kv channel interacting protein 3 (KChIP3) from rabbit cerebellar granule cells.

PA1-928 has been successfully used in immunohistochemistry procedures. Immunohistochemical staining of KChIP3 in rabbit granule cells yields a punctate staining pattern at the plasma membrane.

The PA1-928 synthetic peptide corresponds to amino acid residues 30-45 from mouse KChIP3. This sequence is completely conserved in rat and 87% conserved in humans. The PA1-928 immunizing peptide (Cat. # PEP-161) is available for use in neutralization and control experiments.

Target Information

Voltage-gated potassium (Kv) channels are key components of the potassium currents in the heart and central nervous system. In the heart, Kv4 channels are involved in the repolarization phase of the action potential. In the brain however, these channels prevent reverse-propagation of action potentials. Found associated with Kv4 channels are a group of calcium-binding proteins termed KChIPs (Kv channel interacting proteins). KChIPs are small molecular weight proteins that bind to the cytoplasmic amino termini of Kv4 alpha-subunits and help to modulate its function. There are currently three known KChIPs; KChIP1 expressed in the brain; KChIP2 expressed in the brain, heart, and lung, and has three isoforms, a, b, and c; and KChIP3, also known as calsenilin and DREAM (downstream regulatory elements (DRE)-antagonist modulator) which is expressed in the brain and testis. KChIP3 has activities beyond the modulation of Kv4 currents. It has been shown that KChIP3 binds to the regulatory DRE and represses transcription from the early-response gene c-fos. Thus, KCHiP3 is the first known calcium-binding protein to function as a DNA-binding transcriptional regulator. KChIP3 is also found associated with the presenilins and is implicated in the mediation of beta-amyloid formation.