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Invitrogen
KDEL Polyclonal Antibody
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FIGURE: 1 / 18
KDEL Antibody (PA1-013) in ICC/IF
Immunofluorescent analysis of KDEL in U251 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a KDEL polyclonal antibody (Product # PA1-013) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552). KDEL staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-013
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Hamster,
Human,
Mouse,
Rat
Published species
Hamster,
Human,
Mouse,
Non-human primate,
Rat,
Sea urchin
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-013 detects KDEL from human, rat, mouse and hamster.
PA1-013 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a 57 kDa protein representing protein disulfide isomerase (PDI), a 78 kDa protein representing glucose regulated protein 70 kDa (GRP78) and an ~94 kDa protein representing GRP94 in mouse myeloma cells. Immunofluorescence staining of GRP78 in mouse myeloma cells with PA1-013 results in diffuse cytoplasmic staining consistent with specific ER localization. PA1-013 immunoprecipitates PDI, GRP78, an ~72 kDa protein which may be endoplasmic reticulum protein 72 kDa (ERp72), and an ~94 kDa protein representing GRP94 from numerous rodent tissues.
The PA1-013 immunogen is a synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94.
Reconstitute in 100 µL PBS to create a stock of 1 mg/mL.
Target Information
The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins, GRP78 and GRP94 respectively, and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 78 kDa glucose-regulated protein; 94 kDa glucose-regulated protein; binding-immunoglobulin protein; BiP; chaperone; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; endoplasmic reticulum resident protein 99; endoplasmic reticulum transmembrane protein precursor; Endoplasmin; endothelial cell (HBMEC) glycoprotein; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; epididymis secretory sperm binding protein Li 89n; ER lumen protein retaining receptor 1; glucose regulated protein, 78 kDa; glucose-regulated protein; glucose-regulated protein (grp78); glucose-regulated protein 78; glucose-regulated protein, 78kDa; GRP-78; GRP-94; GRP94; heat shock 70 kDa protein 5; heat shock 70kD protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5 (glucose-regulated protein); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); heat shock protein 5; heat shock protein 70 family protein 5; heat shock protein 90 kDa beta member 1; heat shock protein 90, beta (Grp94), member 1; heat shock protein 90, beta, member 1; heat shock protein 90kDa beta (Grp94), member 1; heat shock protein 90kDa beta family member 1; heat shock protein family A member 5; heat shock protein family C member 4; HSP70 family protein 5; immunoglobulin heavy chain binding protein; immunoglobulin heavy chain-binding protein; KDEL; KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1; KDEL (Lys-Asp-Glu-Leu) receptor; KDELR1; KDEL endoplasmic reticulum protein retention receptor 1; KDEL receptor 1; Lys-Asp-Glu-Leu; polymorphic tumor rejection antigen 1; polymorphic tumor rejection antigen precursor; preimmunoglobulin heavy chain binding protein; putative MAPK-activating protein PM23; RP23-446N16.1; steroidogenesis-activator polypeptide; stress-inducible tumor rejection antigen gp96; transforming growth factor alpha regulated gene 2; tumor rejection antigen (gp96) 1; tumor rejection antigen 1; tumor rejection antigen gp96; unnamed protein product; XAP-1 antigen
Gene Aliases: 8030486F04Rik; baffled; BIP; D2Wsu141e; D2Wsu17e; ECGP; endoplasmin; ERD2; ERD2.1; ERp99; GP96; GRP 78; GRP78; GRP94; HDEL; HEL-S-125m; HEL-S-89n; HEL35; Hsce70; I79_020931; mBiP; PM23; SEZ-7; Sez7; TA-3; Targ2; Tra-1; TRA1
RNA binding
protein phosphatase inhibitor activity
calcium ion binding
protein binding
ATP binding
ATPase activity
protein phosphatase binding
AP-3 adaptor complex binding
protein binding involved in protein folding
low-density lipoprotein particle receptor binding
ATP-dependent protein folding chaperone
enzyme binding
protein domain specific binding
protein serine/threonine kinase inhibitor activity
heat shock protein binding
ubiquitin protein ligase binding
ribosome binding
cadherin binding
chaperone binding
misfolded protein binding
protein sequestering activity
KDEL sequence binding
ER retention sequence binding
Hsp90 family chaperone
membrane trafficking regulatory protein
response to hypoxia
protein folding
response to hormone
protein transport
positive regulation of Wnt signaling pathway
retrograde protein transport, ER to cytosol
actin rod assembly
positive regulation of toll-like receptor signaling pathway
protein folding in endoplasmic reticulum
response to endoplasmic reticulum stress
ERAD pathway
negative regulation of apoptotic process
cellular response to manganese ion
cellular response to ATP
protein localization to plasma membrane
cerebellum structural organization
cerebellar Purkinje cell layer development
substantia nigra development
positive regulation of cell migration
endoplasmic reticulum unfolded protein response
posttranslational protein targeting to membrane, translocation
negative regulation of protein complex assembly
positive regulation of protein ubiquitination
maintenance of protein localization in endoplasmic reticulum
IRE1-mediated unfolded protein response
protein refolding
cellular response to glucose starvation
positive regulation of protein catabolic process
positive regulation of transcription from RNA polymerase II promoter
regulation of protein folding in endoplasmic reticulum
regulation of ATF6-mediated unfolded protein response
regulation of IRE1-mediated unfolded protein response
negative regulation of IRE1-mediated unfolded protein response
regulation of PERK-mediated unfolded protein response
negative regulation of PERK-mediated unfolded protein response
insulin processing
luteolysis
ER overload response
positive regulation of neuron projection development
neuron differentiation
negative regulation of transforming growth factor beta receptor signaling pathway
response to cocaine
neuron apoptotic process
cellular response to antibiotic
cellular response to calcium ion
cellular response to cAMP
cellular response to interleukin-4
cellular response to xenobiotic stimulus
cellular response to gamma radiation
stress response to metal ion
response to methamphetamine hydrochloride
cellular response to nerve growth factor stimulus
translational initiation
PERK-mediated unfolded protein response
positive regulation of embryonic development
protein retention in ER lumen
retrograde vesicle-mediated transport, Golgi to ER
T cell differentiation
T cell apoptotic process
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