Invitrogen

KDM6A Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (13) KDM6A antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

KDM6A Antibody in Western Blot (WB) — Knockdown of KDM6A was achieved by transfecting MCF7 with KDM6A specific siRNAs (Silencer® select Product # s531230; s531232). Western blot analysis (Fig. a) was performed using whole cell extracts from the KDM6A knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with KDM6A Polyclonal Antibody (Product # PA5-31772, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to KDM6A.

KDM6A Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF7 cells were transfected with KDM6A siRNA and reduction of signal was observed in Western Blot using KDM6A Polyclonal Antibody (Product # PA5-31772).

Product Details

PA5-31772

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1106 and 1368 of Human KDM6A

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2549245

Product Specific Information

Recommended positive controls: NT2D1, IMR32, U87-MG.

Predicted reactivity: Mouse (99%), Xenopus laevis (89%), Dog (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: [histone H3]-trimethyl-L-lysine(27) demethylase 6A; bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX)); Histone demethylase UTX; lysine (K)-specific demethylase 6A; Lysine-specific demethylase 6A; ubiquitously transcribed tetratricopeptide repeat protein X-linked; ubiquitously-transcribed TPR gene on the X chromosome; Ubiquitously-transcribed TPR protein on the X chromosome; Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein

Gene Aliases: bA386N14.2; KABUK2; KDM6A; UTX

UniProt ID: (Human) O15550

Entrez Gene ID: (Human) 7403

Molecular Function: chromatin/chromatin-binding, or -regulatory protein