Invitrogen

KDM6A Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

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Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

KDM6A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of KDM6A was performed using 70% confluent log phase K-562 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with KDM6A Polyclonal Antibody (Product # PA5-31828) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear and cytosolic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X with oil immersion magnification.

KDM6A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — KDM6A Polyclonal Antibody detects KDM6A protein at nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. KDM6A Polyclonal Antibody (Product # PA5-31828) dilution: 1:1,000. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

KDM6A Antibody in Western Blot (WB) — Western blot analysis of UTX/KDM6A was performed by loading 2 µg of molecular weight marker (MWM), 40 µg of ARD (a KDM6A negative cell line) or 40 µg of ARP-1 (a matched KDM6A positive cell line) cell lysate onto a NuPAGE 4-12% Bis-Tris Gel (Product # NP0321BOX) and running with Novex NuPAGE MES SDS Running buffer (Product # NP0002). Proteins were transferred to Immobulon-FL PVDF membrane and blocked for 1 hour in LI-COR blocking buffer. Membrane was incubated overnight at 4°C in LI-COR blocking buffer containing a 1:1000 dilution of KDM6A polyclonal antibody (Product # PA5-31828), followed by a 1 hour room temperature incubation in TBST containing a 1:10000 dilution of 680 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 680 (Product # A32734). Detection of KDM6A at approximately 150 kDa (indicated by the arrow) was performed using a LI-COR Odyssey CLx using the 700nm channel. (left) red channel image (right) inverted grey scale image. Data courtesy of Dr. Jonathan D. Licht's laboratory.

KDM6A Antibody in Immunoprecipitation (IP) — KDM6A Polyclonal Antibody immunoprecipitates KDM6A protein in IP experiments. IP samples: SK-N-SH whole cell extract. A. 30 µg SK-N-SH whole cell extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of KDM6A protein by 4 µg KDM6A Polyclonal Antibody (Product # PA5-31828). 5 % SDS-PAGE. The immunoprecipitated KDM6A protein was detected by KDM6A Polyclonal Antibody (Product # PA5-31828) diluted at 1:500.

KDM6A Antibody in ChIP assay (ChIP) — ChIP assay analysis of KDM6A in HeLa chromatin extracts using 5 µg of either normal rabbit IgG or KDM6A Polyclonal Antibody (Product # PA5-31828). The precipitated DNA was detected by PCR with primer set targeting to RBL1 promoter.

KDM6A Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. K-562 cells were transfected with KDM6A siRNA and decrease in signal intensity was observed in Western Blot application using Anti-KDM6A Polyclonal Antibody (Product # PA5-31828). {KD}

Product Details

PA5-31828

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
Immunoprecipitation (IP)	1:100-1:500	-
ChIP assay (ChIP)	Assay-Dependent	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 409 and 750 of Human KDM6A View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.68 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2549301

Product Specific Information

Recommended positive controls: SK-N-SH, IMR32, SK-N-AS.

Predicted reactivity: Mouse (93%), Xenopus laevis (82%), Dog (86%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 4lysine (K)-specific demethylase 6A; [histone H3]-trimethyl-L-lysine(27) demethylase 6A; bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX)); Histone demethylase UTX; lysine (K)-specific demethylase 6A; Lysine-specific demethylase 6A; ubiquitously transcribed tetratricopeptide repeat gene, X chromosome; ubiquitously transcribed tetratricopeptide repeat protein X-linked; Ubiquitously transcribed TPR protein on the X chromosome; Ubiquitously transcribed X chromosome tetratricopeptide repeat protein; ubiquitously-transcribed TPR gene on the X chromosome; Ubiquitously-transcribed TPR protein on the X chromosome; Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein

Gene Aliases: bA386N14.2; KABUK2; KDM6A; UTX

UniProt ID: (Human) O15550, (Mouse) O70546

Entrez Gene ID: (Human) 7403, (Mouse) 22289

Molecular Function: chromatin/chromatin-binding, or -regulatory protein