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Invitrogen
KDM6A Monoclonal Antibody (GT1612)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
KDM6A Antibody (MA5-27791) in ICC/IF
Immunofluorescence analysis of KDM6A was performed using 70% confluent log phase K-562 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with KDM6A Monoclonal Antibody (GT1612) (Product # MA5-27791) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766),... View More
Product Details
MA5-27791
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
GT1612
Immunogen
Recombinant protein encompassing a sequence within the center region of human KDM6A. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2735252
Product Specific Information
Positive Control: U87-MG, SK-N-SH, IMR32, SK-N-AS
Predicted Reactivity: Mouse (93%), Xenopus laevis (82%), Dog (86%), Bovine (98%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 4lysine (K)-specific demethylase 6A; [histone H3]-trimethyl-L-lysine(27) demethylase 6A; bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX)); Histone demethylase UTX; lysine (K)-specific demethylase 6A; Lysine-specific demethylase 6A; ubiquitously transcribed tetratricopeptide repeat gene, X chromosome; ubiquitously transcribed tetratricopeptide repeat protein X-linked; Ubiquitously transcribed TPR protein on the X chromosome; Ubiquitously transcribed X chromosome tetratricopeptide repeat protein; ubiquitously-transcribed TPR gene on the X chromosome; Ubiquitously-transcribed TPR protein on the X chromosome; Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein
Gene Aliases: bA386N14.2; KABUK2; KDM6A; UTX
UniProt ID: (Human) O15550, (Mouse) O70546
Entrez Gene ID: (Human) 7403, (Mouse) 22289
RNA polymerase II core promoter proximal region sequence-specific DNA binding
chromatin DNA binding
histone demethylase activity
identical protein binding
metal ion binding
dioxygenase activity
histone demethylase activity (H3-K27 specific)
sulfonate dioxygenase activity
chromatin binding
protein binding
oxidoreductase activity
2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity
procollagen-proline dioxygenase activity
hypophosphite dioxygenase activity
DNA-N1-methyladenine dioxygenase activity
C-19 gibberellin 2-beta-dioxygenase activity
C-20 gibberellin 2-beta-dioxygenase activity
chromatin/chromatin-binding, or -regulatory protein
in utero embryonic development
neural tube closure
heart morphogenesis
respiratory system process
positive regulation of gene expression
somite rostral/caudal axis specification
multicellular organism growth
mesodermal cell differentiation
notochord morphogenesis
histone H3-K4 methylation
oxidation-reduction process
canonical Wnt signaling pathway
histone H3-K27 demethylation
heart development
regulation of gene expression
chromatin modification
neural tube development
embryonic organ development
cardiovascular system development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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