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KGF Antibody (PA5-115465) in ICC/IF
Immunocytochemistry analysis of KGF in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115465) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115465
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human FGF7(Accession P21781), corresponding to amino acid residues L30-D80.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2900101
Product Specific Information
Antibody detects endogenous levels of total KGF.
Target Information
Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FGF-7; Fibroblast growth factor 7; HBGF-7; Heparin-binding growth factor 7; Keratinocyte growth factor; KGF
Gene Aliases: Fgf-7; FGF7; HBGF-7; KGF
UniProt ID: (Human) P21781, (Rat) Q02195, (Mouse) P36363
Entrez Gene ID: (Human) 2252, (Rat) 29348, (Mouse) 14178
protein tyrosine kinase activity
Ras guanyl-nucleotide exchange factor activity
type 2 fibroblast growth factor receptor binding
protein binding
growth factor activity
heparin binding
1-phosphatidylinositol-3-kinase activity
chemoattractant activity
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
fibroblast growth factor receptor binding
receptor binding
growth factor
intercellular signal molecule
MAPK cascade
ovarian follicle development
positive regulation of defense response to virus by host
phosphatidylcholine biosynthetic process
signal transduction
positive regulation of cell proliferation
fibroblast growth factor receptor signaling pathway
epidermis development
response to wounding
mesenchymal cell proliferation
positive regulation of keratinocyte proliferation
regulation of phosphatidylinositol 3-kinase signaling
peptidyl-tyrosine phosphorylation
hair follicle morphogenesis
actin cytoskeleton reorganization
protein localization to cell surface
phosphatidylinositol-3-phosphate biosynthetic process
wound healing
surfactant homeostasis
positive regulation of GTPase activity
positive regulation of transcription, DNA-templated
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
positive regulation of epithelial cell proliferation
positive regulation of peptidyl-tyrosine phosphorylation
positive chemotaxis
positive regulation of keratinocyte migration
positive regulation of cell division
branching involved in salivary gland morphogenesis
positive regulation of epithelial cell proliferation involved in lung morphogenesis
regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling
secretion by lung epithelial cell involved in lung growth
xenophagy
phospholipid biosynthetic process
lung development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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