Invitrogen

KHSRP Polyclonal Antibody

View all (15) KHSRP antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (4)

FIGURE: 1 / 4

KHSRP Antibody (PA5-103840) in WB

Western blot analysis of KHSRP in various samples (Lane 1: 3T3, treated with blocking peptide, Lane 2: 3T3, Lane 3: RAW2647 cells). Samples were incubated with KHSRP polyclonal antibody (Product # PA5-103840).

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KHSRP Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of KHSRP in HepG2 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and KHSRP polyclonal antibody (Product # PA5-103840) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

KHSRP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded KHSRP in rat heart tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with KHSRP polyclonal antibody (Product # PA5-103840) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Product Details

PA5-103840

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human KHSRP, corresponding to a region within N-terminal amino acids.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

RRID

AB_2853173

Target Information

The KHSRP gene encodes a multifunctional RNA-binding protein implicated in a variety of cellular processes, including transcription, alternative pre-mRNA splicing, and mRNA localization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Far upstream element-binding protein 2; FUSE binding protein 2; FUSE-binding protein 2; KH type-splicing regulatory protein; KHE3; KSRP; MAP2 RNA trans-acting protein 1; MAP2 RNA trans-acting protein MARTA1; MARTA1; p75

Gene Aliases: 6330409F21Rik; FBP2; FUBP2; KHSRP; KSRP; Marta1

UniProt ID: (Human) Q9UNT5, (Rat) Q99PF5, (Mouse) Q3U0V1

Entrez Gene ID: (Human) 8570, (Rat) 171137, (Mouse) 16549

Molecular Function: RNA metabolism protein