Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 5
KIF14 Antibody (PA5-87769) in WB
Western blot analysis of KIF14 in extracts of various cell lines. Samples were incubated with KIF14 Polyclonal antibody (Product # PA5-87769) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Product Details
PA5-87769
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1399-1648 of human KIF14
Conjugate
Unconjugated
Form
Liquid
Concentration
2.46 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_2804396
Product Specific Information
Immunogen sequence: LENGNNKAAS VQEEFMDAVC DGVGLGMKIL LDSGLEKAKE LQHELFRQCT KNEVTKEMKT NAMGLIRSLE NIFAESKIKS FRRQVQEENF EYQDFKRMVN RAPEFLKLKH CLEKAIEIII SALKGCHSDI NLLQTCVESI RNLASDFYSD FSVPSTSVGS YESRVTHIVH QELESLAKSL LFCFESEESP DLLKPWETYN QNTKEEHQQS KSSGIDGSKN KGVPKRVYEL HGSSPAVSSE ECTPSRIQWV; Positive Samples: HeLa, HL-60; Cellular Location: Cytoplasm, Midbody, Nucleus, cytoskeleton, spindle
Target Information
This gene encodes a member of the kinesin-3 superfamily of microtubule motor proteins. These proteins are involved in numerous processes including vesicle transport, chromosome segregation, mitotic spindle formation, and cytokinesis. In human HeLa-S3 and 293T cells, this protein is localized to the cytoplasm during interphase, to the spindle poles and spindle microtubules during mitosis, and to the midbody during cytokinesis. An internal motor domain displays microtubule-dependent ATPase activity, consistent with its function as a microtubule motor protein. Knockdown of this gene results in failed cytokinesis with endoreplication, which results in multinucleated cells. This gene has been identified as a likely oncogene in breast, lung and ovarian cancers, as well as retinoblastomas and gliomas. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Kinesin-like protein KIF14; N-3 kinesin; truncated kinesin family member 14
Gene Aliases: D1Ertd367e; E130203M01; KIAA0042; KIF14; MKS12
UniProt ID: (Human) Q15058, (Mouse) L0N7N1
Entrez Gene ID: (Human) 9928, (Mouse) 381293, (Rat) 360849
Molecular Function:
microtubule binding motor protein
non-motor actin binding protein
regulation of cell growth
microtubule-based movement
microtubule depolymerization
mitotic metaphase plate congression
positive regulation of cell proliferation
regulation of G2/M transition of mitotic cell cycle
cerebellar granular layer structural organization
cerebellar Purkinje cell layer structural organization
cerebellar cortex development
hippocampus development
olfactory bulb development
cell proliferation in forebrain
cerebral cortex development
regulation of cell adhesion
regulation of cell migration
cytoskeleton-dependent intracellular transport
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
regulation of myelination
activation of protein kinase activity
positive regulation of cytokinesis
regulation of Rap protein signal transduction
negative regulation of integrin activation
substrate adhesion-dependent cell spreading
negative regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of neuron apoptotic process
negative regulation of neuron apoptotic process
establishment of protein localization
cell division
response to docetaxel trihydrate
mitotic cell cycle process
regulation of cell maturation
regulation of G1/S transition of mitotic cell cycle
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support