Invitrogen

KIF15 Polyclonal Antibody

KIF15 Antibody in Immunohistochemistry (IHC)

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KIF15 Antibody (PA5-57305) in IHC

Immunohistochemical staining of KIF15 in human small intestine using KIF15 Polyclonal Antibody (Product # PA5-57305) shows moderate to strong cytoplasmic positivity in glandular cells.

Product Image Gallery

KIF15 Antibody — Figure 2 KIF15 expression was effectively inhibited in both Hep3B and SNU-475 human HCC cells caused by its shRNA plasmids. (a) Quantitative PCR assays revealed the dramatically reduced expression level of KIF15 caused by its shRNA in Hep3B and SNU-475 cells, respectively. (b) Immunoblot assays confirmed the efficiently silencing of KIF15 caused by the transfection of its shRNA plasmids in Hep3B and SNU-475 cells. Results are presented as mean ± SD, *P < 0.05. {KD}

Product Details

PA5-57305

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:50

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human KIF15. Recombinant protein control fragment (Product #RP-96412).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2643086

Product Specific Information

Immunogen sequence: ESLLATEKVI SSLEKSRDSD KKVVADLMNQ IQELRTSVCE KTETIDTLKQ ELKDINCKYN SALVDREESR VLIKKQEVDI LD

Highest antigen sequence identity to the following orthologs: Mouse - 74%, Rat - 70%.

Target Information

Plus-end directed kinesin-like motor enzyme involved in mitotic spindle assembly. Target protein's mouse homolog associates with microtubulew and appears to play a role in mitosis in fibroblasts and in migration in developing neurons.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

KIF15 Promotes Proliferation and Growth of Hepatocellular Carcinoma.

Analytical cellular pathology (Amsterdam)

Sun YF,Wu HL,Shi RF,Chen L,Meng C

PA5-57305 was used in Immunohistochemistry to confirm that KIF15 could be a novel therapeutic target for the treatment of hepatocellular carcinoma (HCC).

Wed Jan 13 00:00:00 EST 2021

KIF15 Promotes Proliferation and Growth of Hepatocellular Carcinoma.

Analytical cellular pathology (Amsterdam)

Sun YF,Wu HL,Shi RF,Chen L,Meng C

PA5-57305 was used in Immunohistochemistry to confirm that KIF15 could be a novel therapeutic target for the treatment of hepatocellular carcinoma (HCC).

Wed Jan 13 00:00:00 EST 2021

Bioinformatics

Protein Aliases: hKLP2; kinesin-like 7; Kinesin-like protein 2; Kinesin-like protein 7; Kinesin-like protein KIF15; Serologically defined breast cancer antigen NY-BR-62

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Gene Aliases: HKLP2; KIF15; KLP2; KNSL7; NY-BR-62

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UniProt ID: (Human) Q9NS87

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Entrez Gene ID: (Human) 56992

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Function(s)

DNA binding motor activity microtubule motor activity ATP binding microtubule binding ATPase activity microtubule binding motor protein

Process(es)

retrograde vesicle-mediated transport, Golgi to ER microtubule-based movement mitotic nuclear division cell proliferation antigen processing and presentation of exogenous peptide antigen via MHC class II

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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KIF15 Polyclonal Antibody

KIF15 Antibody (PA5-57305) in IHC

Product Details

PA5-57305

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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