Invitrogen

KIF18A Polyclonal Antibody

View all (8) KIF18A antibodies

Questions & Answers

Questions & Answers

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KIF18A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of KIF18A in human cell line U-2 OS using a KIF18A Polyclonal Antibody (Product # PA5-58728) shows localization to cytosol, microtubules and cytokinetic bridge.

KIF18A Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of KIF18A in human rectal tissue shows strong cytoplasmic positivity in glandular cells. Samples were probed using a KIF18A Polyclonal Antibody (Product # PA5-58728).

KIF18A Antibody in Western Blot (WB) — Western blot analysis of KIF18A in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a KIF18A Polyclonal Antibody (Product # PA5-58728).

Product Details

PA5-58728

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (IHC)	1:20-1:50	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human KIF18A View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2643089

Product Specific Information

Immunogen sequence: LHCHHLHLQN KDLKAQIRHM MDLACLQEQQ HRQTEAVLNA LLPTLRKQYC TLKEAGLSNA AFESDFKEIE HLVERKKVVV WADQTAEQPK QND

Highest antigen sequence identity to the following orthologs: Mouse - 80%, Rat - 82%.

Target Information

May have a role in a signaling pathway. Could act as a tumor suppressor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Kinesin-like protein KIF18A; Marrow stromal KIF18A; MS-KIF18A; protein phosphatase 1, regulatory subunit 99

Gene Aliases: KIF18A; MS-KIF18A; OK/SW-cl.108; PPP1R99

UniProt ID: (Human) Q8NI77

Entrez Gene ID: (Human) 81930

Molecular Function: microtubule binding motor protein

retrograde vesicle-mediated transport, Golgi to ER microtubule-based movement microtubule depolymerization sister chromatid cohesion mitotic metaphase plate congression male meiosis protein transport antigen processing and presentation of exogenous peptide antigen via MHC class II regulation of microtubule cytoskeleton organization seminiferous tubule development

Process(es)

actin binding protein binding ATP binding microtubule binding ATP-dependent microtubule motor activity, plus-end-directed ubiquitin binding microtubule plus-end binding tubulin-dependent ATPase activity