|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 51 and 294 of Human KIFC3|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
PA5-30074 targets KIFC3 in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-30074 immunogen is recombinant fragment corresponding to a region within amino acids 51 and 294 of Human KIFC3.
KIFC3 belongs to the large superfamily of kinesins, molecular motors that use the energy of ATP hydrolysis to translocate cargoes along microtubules. Members share extensive homology within a globular domain containing the microtubule- and ATP-binding sites and have a coiled-coil stalk domain that mediates oligomerization. Different kinesin family members participate in specific and diverse motile processes, such as cell division, organelle transport, and nuclear movement (Hoang et al., 1998 [PubMed 9782090]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
KIFC3 promotes mitotic progression and integrity of the central spindle in cytokinesis.
PA5-30074 was used in immunocytochemistry and western blot to demonstrate that KIFC3 is important for central bridge morphology
|Nachbar J,Lázaro-Diéguez F,Prekeris R,Cohen D,Müsch A||Cell cycle (Georgetown, Tex.) (13:426)||2014|