Proteintech

KL Polyclonal Antibody

View all (14) Klotho antibodies

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KL Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28100-1-AP (KL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KL Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28100-1-AP (KL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KL Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 28100-1-AP (KL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

KL Antibody in Immunoprecipitation (IP) — IP result of anti-KL (IP:28100-1-AP, 4 µg; Detection:28100-1-AP 1:300) with mouse kidney tissue lysate 4000 µg.

Product Details

28100-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:200	-
Immunoprecipitation (IP)	1:300	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KL Fusion Protein Ag27759
Conjugate	Unconjugated
Form	Liquid
Concentration	233 µg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

KLOTHO is the systemic anti-aging hormone within the glycosidase1 superfamily. It encodes a type I membrane protein that is abundant in the kidney and brain. In mice, a deficiency in KLOTHO expression leads to various systemic phenotypes resembling human aging such as arteriosclerosis, osteoporosis, and skin atrophy together with growth retardation, short life-span and infertility. Transgenic mice overexpressing KLOTHO have an extended life span by inhibiting insulin/IGF1 signaling. KLOTHO is involved in the regulation of calcium/phosphorus homeostasis by inhibiting the synthesis of active vitamin D and identified as a potential tumor suppressor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: HFTC3; Klotho; Klotho peptide; secreted form of Klotho protein

Gene Aliases: alpha-kl; KL

UniProt ID: (Human) Q9UEF7, (Mouse) O35082

Entrez Gene ID: (Human) 9365, (Mouse) 16591

Molecular Function: glycosidase

MAPK cascade carbohydrate metabolic process energy reserve metabolic process aging insulin receptor signaling pathway fibroblast growth factor receptor signaling pathway regulation of phosphatidylinositol 3-kinase signaling positive regulation of bone mineralization phosphatidylinositol-3-phosphate biosynthetic process positive regulation of GTPase activity phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling calcium ion homeostasis positive regulation of MAPKKK cascade by fibroblast growth factor receptor signaling pathway glycosyl compound metabolic process acute inflammatory response metabolic process

Process(es)

beta-glucuronidase activity signal transducer activity Ras guanyl-nucleotide exchange factor activity fibroblast growth factor receptor binding hormone activity vitamin D binding beta-glucosidase activity 1-phosphatidylinositol-3-kinase activity fibroblast growth factor binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity hydrolase activity, hydrolyzing O-glycosyl compounds protein binding hydrolase activity hydrolase activity, acting on glycosyl bonds