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KLF12 Antibody (PA5-120729) in IHC (P)
Immunohistochemistry analysis of KLF12 in paraffin-embedded mouse brain. Samples were incubated with KLF12 Polyclonal antibody (Product # PA5-120729) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Details
PA5-120729
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 220-310 of human KLF12 (NP_009180.3).
Conjugate
Form
Liquid
Concentration
0.47 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914301
Product Specific Information
Positive test controls include: Mouse brain, Rat brain.
Immunogen sequence: GHGKAQMDPR GLSPRQSKSD SDDDDLPNVT LDSVNETGST ALSIARAVQE VHPSPVSRVR GNRMNNQKFP CSISPFSIES TRRQRRSESP D
Target Information
Activator protein-2 alpha (AP-2 alpha) is a developmentally-regulated transcription factor and important regulator of gene expression during vertebrate development and carcinogenesis. The protein encoded by this gene is a member of the Kruppel-like zinc finger protein family and can repress expression of the AP-2 alpha gene by binding to a specific site in the AP-2 alpha gene promoter. Repression by the encoded protein requires binding with a corepressor, CtBP1. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BHLHB24; ID; Krueppel-like factor 12; Transcriptional repressor AP-2rep
Gene Aliases: 2700063E05Rik; AI225908; AI315654; AP-2rep; Ap2rep; B130052C06Rik; D530033K05Rik; Klf12
UniProt ID: (Mouse) O35738
Entrez Gene ID: (Mouse) 16597, (Rat) 306110
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
nucleic acid binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
metal ion binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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