Invitrogen

KLF2 Polyclonal Antibody

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KLF2 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of human brain tissue using an anti-KLF2 polyclonal antibody (Product # PA5-40591).

KLF2 Antibody in Western Blot (WB) — Western blot analysis of Hela whole cell lysate using an anti-KLF2 polyclonal antibody (Product # PA5-40591). Lane 1) Primary Antibody; Lane 2) Primary Antibody + Blocking Peptide.

KLF2 Antibody in ChIP assay (CHIP) — ChIP assay analysis of quiescent human colon carcinoma HCT116 cells using an anti-KLF2 polyclonal antibody (Product # PA5-40591).

KLF2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — FIGURE 3 miR-144 directly targets and negatively regulates KLF2. A, Box plots displaying the expression of KLF2 in normal samples (blue box on the left) and TRALI samples (red box on the right) from microarray data set GSE11434 . B, The mRNA level of KLF2 in blood samples from healthy people and patients with TRALI determined by RT-qPCR. C, Immunohistochemistry to detect the level of KLF2 in lung tissues of patients with TRALI (x400). D, The putative binding sites between miR-144 and KLF2 predicted from the Starbase website. E, The binding relationship between miR-144 and KLF2 verified by a dual-luciferase reporter gene assay. F, The mRNA levels of miR-144 and KLF2 in human blood samples after transfection of miR-144 mimic/inhibitor measured by RT-qPCR. G, KLF2 protein levels in human blood samples after transfection of miR-144 mimic/inhibitor normalized to GAPDH evaluated by Western blot analysis. H, KLF2 mRNA levels in normal and TRALI mouse blood samples after transfection examined by RT-qPCR. I, Pearson correlation analysis of the correlation between miR-144 and KLF2 mRNA expression in mice. The measurement data were expressed by the mean +- standard deviation. The experiment was repeated 3 times independently. The comparison between the two groups was analysed by an unpaired t test. Data among multiple groups were compared using a one-way ANOVA, followed by Tukey's post hoc test. Pearson correlation was used for correlation analysis. * P < .05 vs. healthy donors, the NC

Product Details

PA5-40591

Applications	Tested Dilution	Publications
Western Blot (WB)	0.2-1 µg/mL	View 5 publications 5 publications
Immunohistochemistry (IHC)	Assay-Dependent	-
Immunohistochemistry (Paraffin) (IHC (P))	-	View 1 publication 1 publication
ChIP assay (ChIP)	0.5 µg	-

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide directed towards the middle region of human KLF2
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS with 2% sucrose
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2576301

Product Specific Information

Peptide sequence: PPAFGLFDDA AAAAAALGLA PPAARGLLTP PASPLELLEA KPKRGRRSWP

Sequence homology: Guinea Pig: 93%; Human: 100%; Mouse: 93%; Pig: 100%; Rat: 93%; Zebrafish: 79%

Target Information

KLF2 belongs to the krueppel C2H2-type zinc-finger protein family. It contains 3 C2H2-type zinc fingers. KLF2 binds to the CACCC box in the beta-globin gene promoter and activates transcription.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Intermittent High Glucose Elevates Nuclear Localization of EZH2 to Cause H3K27me3-Dependent Repression of KLF2 Leading to Endothelial Inflammation.

Cells

Thakar S,Katakia YT,Ramakrishnan SK,Pandya Thakkar N,Majumder S

PA5-40591 was used in Western Blot to explore the role of enhancer of zeste homolog 2 and its product histone 3 lysine 27 trimethylation in high glucose-mediated endothelial inflammation.

Sun Sep 26 00:00:00 EDT 2021

Intermittent High Glucose Elevates Nuclear Localization of EZH2 to Cause H3K27me3-Dependent Repression of KLF2 Leading to Endothelial Inflammation.

Cells

Thakar S,Katakia YT,Ramakrishnan SK,Pandya Thakkar N,Majumder S

PA5-40591 was used in Western Blot to explore the role of enhancer of zeste homolog 2 and its product histone 3 lysine 27 trimethylation in high glucose-mediated endothelial inflammation.

Sun Sep 26 00:00:00 EDT 2021

Retinoblastoma cell-derived exosomes promote angiogenesis of human vesicle endothelial cells through microRNA-92a-3p.

Cell death & disease

Chen S,Chen X,Luo Q,Liu X,Wang X,Cui Z,He A,He S,Jiang Z,Wu N,Chen P,Yu K,Zhuang J

PA5-40591 was used in Western Blot to investigate if exosomal miR-92a-3p is involved in tumor angiogenesis and might be a promising therapeutic candidate for retinoblastoma.

Tue Jul 13 00:00:00 EDT 2021

Retinoblastoma cell-derived exosomes promote angiogenesis of human vesicle endothelial cells through microRNA-92a-3p.

Cell death & disease

Chen S,Chen X,Luo Q,Liu X,Wang X,Cui Z,He A,He S,Jiang Z,Wu N,Chen P,Yu K,Zhuang J

PA5-40591 was used in Western Blot to investigate if exosomal miR-92a-3p is involved in tumor angiogenesis and might be a promising therapeutic candidate for retinoblastoma.

Tue Jul 13 00:00:00 EDT 2021

MiR-144-induced KLF2 inhibition and NF-kappaB/CXCR1 activation promote neutrophil extracellular trap-induced transfusion-related acute lung injury.

Journal of cellular and molecular medicine

Le A,Wu Y,Liu W,Wu C,Hu P,Zou J,Kuang L

PA5-40591 was used in Immunohistochemistry (Paraffin) to to explore the role of miR-144 and the molecular mechanisms in neutrophil-extracellular-trap-induced transfusion-related acute lung injury.

Thu Jul 01 00:00:00 EDT 2021

MiR-144-induced KLF2 inhibition and NF-kappaB/CXCR1 activation promote neutrophil extracellular trap-induced transfusion-related acute lung injury.

Journal of cellular and molecular medicine

Le A,Wu Y,Liu W,Wu C,Hu P,Zou J,Kuang L

PA5-40591 was used in Immunohistochemistry (Paraffin) to to explore the role of miR-144 and the molecular mechanisms in neutrophil-extracellular-trap-induced transfusion-related acute lung injury.

Thu Jul 01 00:00:00 EDT 2021

IRF2BP2 prevents ox-LDL-induced inflammation and EMT in endothelial cells via regulation of KLF2.

Experimental and therapeutic medicine

Jiang Y,Shen Q

Published figure using KLF2 polyclonal antibody (Product # PA5-40591) in Western Blot

Sat May 01 00:00:00 EDT 2021

Sex differences in the proliferation of pulmonary artery endothelial cells: implications for plexiform arteriopathy.

Journal of cell science

Qin S,Predescu DN,Patel M,Drazkowski P,Ganesh B,Predescu SA

PA5-40591 was used in Western Blotting to demonstrate that using PAECs engineered to express tagged versions of EHITSN and SH3A-EITSN, the two ITSN fragments increase both p38-ELK1 activation and the ratio of p38 to ERK1 and ERK2 activity, leading to PAEC proliferation, with female cells being more responsive than male cells.

Thu May 14 00:00:00 EDT 2020

Diagnostic Utility and Pathogenic Role of Circulating MicroRNAs in Vasospastic Angina.

Journal of clinical medicine

Park CS,Kim I,Oh GC,Han JK,Yang HM,Park KW,Cho HJ,Kang HJ,Koo BK,Chung WY,Oh S,Lee HY

PA5-40591 was used in Western Blotting to investigate the diagnostic value and pathophysiological role of circulating microRNA in vasospastic angina.

Sat May 02 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: Krueppel-like factor 2; Kruppel-like factor 2 (lung); Kruppel-like factor LKLF; Lung krueppel-like factor; lung Kruppel-like factor; lung Kruppel-like zinc finger transcription factor

Gene Aliases: KLF2; LKLF

UniProt ID: (Human) Q9Y5W3

Entrez Gene ID: (Human) 10365

Molecular Function: C2H2 zinc finger transcription factor