The predicted molecular weight of KLF4 is ~55kD. The migration of this protein by SDS-PAGE was observed slightly higher (~60kD) with both PA1-095 and a benchmark antibody.
PA1-095 detects a predominant band at ~60kD, and a nonspecific band of unknown origin at ~80kD in both whole cell lysates and nuclear extracts.
For best results, StartingBlock T20 (TBS) Blocking Buffer (Product # 37543) is recommended.
KLF4 (Kruppel-like factor 4 (gut)) is a transcription factor that can act both as an activator and as a repressor. KLF4 binds to the 5'-CACCC-3' core sequence, and binds to the promoter region of its own gene and it can activate its own transcription. It regulates the expression of key transcription factors during embryonic development. KLF4 plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. It is required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. It is also involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Diseases associated with KLF4 include skin sequamous cell carcinoma and familial adenomatous polyposis.
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Protein Aliases: endothelial Kruppel-like zinc finger protein; Epithelial Zinc Finger Protein EZF; EZF; GKLF; gut Kruppel-like factor; Gut-enriched krueppel-like factor; Gut-Enriched Kruppel-Like Factor; Krueppel-like factor 4; Kruppel-like factor 4 (Gut enriched kruppel-like factor) (Epithelial zinc-finger protein EZF); Kruppel-Like Factor 4 (Gut)
Gene Aliases: EZF; GKLF; KLF4; Zie