Proteintech

KLF5 Polyclonal Antibody

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Antibody Testing Data (14)

KLF5 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

KLF5 Antibody (21017-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 21017-1-AP (KLF5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

KLF5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

KLF5 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

21017-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 28 publications 28 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 7 publications 7 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 3 publications 3 publications

ChIP assay (ChIP)

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLF5 Fusion Protein Ag15267 (1-350 aa encoded by BC042131)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody detects the 50-60 kDa isoform 1 of KLF5. The antibody can recognize isoform 2 (33 kDa) and isoform 3 (18 kDa) too.

Immunogen sequence: MATRVLSMS ARLGPVPQPP APQDEPVFAQ LKPVLGAANP ARDAALFPGE ELKHAHHRPQ AQPAPAQAPQ PAQPPATGPR LPPEDLVQTR CEMEKYLTPQ LPPVPIIPEH KKYRRDSASV VDQFFTDTEG LPYSINMNVF LPDITHLRTG LYKSQRPCVT HIKTEPVAIF SHQSETTAPP PAPTQALPEF TSIFSSHQTA APEVNNIFIK QELPTPDLHL SVPTQQGHLY QLLNTPDLDM PSSTNQTAAM DTLNVSMSAA MAGLNTHTSA VPQTAVKQFQ GMPPCTYTMP SQFLPQQATY FPPSPPSSEP GSPDRQAEML QNLTPPPSYA ATIASKLAIH NPNLPTTLPV N (1-350 aa encoded by BC042131)

Target Information

KLF5 is a member of the Kruppel-like factor subfamily of zinc finger proteins. It contains three C2H2-type zinc fingers at the carboxyl-terminus that preferentially bind to cis-DNA elements that are GC rich. KLF5 is a transcription activator that promotes cell proliferation and oncogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

KLF5 and p53 comprise an incoherent feed-forward loop directing cell-fate decisions following stress.

Cell death & disease

Yang Y,Bhargava D,Chen X,Zhou T,Dursuk G,Jiang W,Wang J,Zong Z,Katz SI,Lomberk GA,Urrutia RA,Katz JP

Tue May 02 00:00:00 EDT 2023

KLF5 and p53 comprise an incoherent feed-forward loop directing cell-fate decisions following stress.

Cell death & disease

Yang Y,Bhargava D,Chen X,Zhou T,Dursuk G,Jiang W,Wang J,Zong Z,Katz SI,Lomberk GA,Urrutia RA,Katz JP

Tue May 02 00:00:00 EDT 2023

Epithelial cells activate fibroblasts to promote esophageal cancer development.

Cancer cell

Chen Y,Zhu S,Liu T,Zhang S,Lu J,Fan W,Lin L,Xiang T,Yang J,Zhao X,Xi Y,Ma Y,Cheng G,Lin D,Wu C

Mon May 08 00:00:00 EDT 2023

Refined expression quantitative trait locus analysis on adenocarcinoma at the gastroesophageal junction reveals susceptibility and prognostic markers.

Frontiers in genetics

Zhong C,Wu C,Lin Y,Lin D

Sun Jun 04 00:00:00 EDT 2023

Downregulation of miR‑7 and miR‑153 is involved in Helicobacter pylori CagA induced gastric carcinogenesis and progression.

International journal of oncology

Song Y,Guo D,Liu JF,Ge LN,Liu P,Qu YM,Cong HY,Li T,Chang X,Wang YR,Shao LY,Du ZJ,Wang MY

Sat Jul 01 00:00:00 EDT 2023

STK24 Promotes Progression of LUAD and Modulates the Immune Microenvironment.

Mediators of inflammation

Li Y,Liu Y,Wang K,Xue D,Huang Y,Tan Z,Chen Y

Tue May 16 00:00:00 EDT 2023

Nitazoxanide inhibits acetylated KLF5-induced bone metastasis by modulating KLF5 function in prostate cancer.

BMC medicine

Huang Q,Liu M,Zhang D,Lin BB,Fu X,Zhang Z,Zhang B,Dong JT

Tue Feb 21 00:00:00 EST 2023

Nitazoxanide inhibits acetylated KLF5-induced bone metastasis by modulating KLF5 function in prostate cancer.

BMC medicine

Huang Q,Liu M,Zhang D,Lin BB,Fu X,Zhang Z,Zhang B,Dong JT

Tue Feb 21 00:00:00 EST 2023

A WWP2-PTEN-KLF5 signaling axis regulates odontoblast differentiation and dentinogenesis in mice.

The Journal of biological chemistry

Fu J,Zhang X,Zheng H,Yang G,Chen Z,Yuan G

Mon Aug 01 00:00:00 EDT 2022

A WWP2-PTEN-KLF5 signaling axis regulates odontoblast differentiation and dentinogenesis in mice.

The Journal of biological chemistry

Fu J,Zhang X,Zheng H,Yang G,Chen Z,Yuan G

Mon Aug 01 00:00:00 EDT 2022

A WWP2-PTEN-KLF5 signaling axis regulates odontoblast differentiation and dentinogenesis in mice.

The Journal of biological chemistry

Fu J,Zhang X,Zheng H,Yang G,Chen Z,Yuan G

Mon Aug 01 00:00:00 EDT 2022

Integrated multi-omics analyses identify key anti-viral host factors and pathways controlling SARS-CoV-2 infection.

Research square

Hou J,Wei Y,Zou J,Jaffery R,Liang S,Zheng C,Chen K,Shi PY,Chen Y,Xie X,Peng W

Mon Aug 15 00:00:00 EDT 2022

Histamine Deficiency Promotes Myofibroblasts Transformation from HDC-Expressing CD11b⁺ Myeloid Cells in Injured Hearts Post Myocardial Infarction.

Journal of cardiovascular translational research

Zhu B,Zhu X,Wang X,Wu J,Ding S,Zhang W,Zou Y,Ge J,Yang X

Wed Jun 01 00:00:00 EDT 2022

Histamine Deficiency Promotes Myofibroblasts Transformation from HDC-Expressing CD11b⁺ Myeloid Cells in Injured Hearts Post Myocardial Infarction.

Journal of cardiovascular translational research

Zhu B,Zhu X,Wang X,Wu J,Ding S,Zhang W,Zou Y,Ge J,Yang X

Wed Jun 01 00:00:00 EDT 2022

Induction of GLI1 by miR-27b-3p/FBXW7/KLF5 pathway contributes to pulmonary arterial hypertension.

Journal of molecular and cellular cardiology

Wang Q,Chai L,Zhang Q,Wang J,Liu J,Chen H,Wang Y,Chen Y,Shen N,Xie X,Li M

Sat Oct 01 00:00:00 EDT 2022

Inhibitory effect of the novel tyrosine kinase inhibitor DCC-2036 on triple-negative breast cancer stem cells through AXL-KLF5 positive feedback loop.

Cell death & disease

Shen Y,Zhu Q,Xiao M,Yin L,Feng W,Feng J,He J,Li P,Chen X,Ding W,Zhong J,Zeng Z,Xie Z,Liu J,Zu X

Tue Aug 30 00:00:00 EDT 2022

Inhibitory effect of the novel tyrosine kinase inhibitor DCC-2036 on triple-negative breast cancer stem cells through AXL-KLF5 positive feedback loop.

Cell death & disease

Shen Y,Zhu Q,Xiao M,Yin L,Feng W,Feng J,He J,Li P,Chen X,Ding W,Zhong J,Zeng Z,Xie Z,Liu J,Zu X

Tue Aug 30 00:00:00 EDT 2022

Inhibitory effect of the novel tyrosine kinase inhibitor DCC-2036 on triple-negative breast cancer stem cells through AXL-KLF5 positive feedback loop.

Cell death & disease

Shen Y,Zhu Q,Xiao M,Yin L,Feng W,Feng J,He J,Li P,Chen X,Ding W,Zhong J,Zeng Z,Xie Z,Liu J,Zu X

Tue Aug 30 00:00:00 EDT 2022

Inhibitory effect of the novel tyrosine kinase inhibitor DCC-2036 on triple-negative breast cancer stem cells through AXL-KLF5 positive feedback loop.

Cell death & disease

Shen Y,Zhu Q,Xiao M,Yin L,Feng W,Feng J,He J,Li P,Chen X,Ding W,Zhong J,Zeng Z,Xie Z,Liu J,Zu X

Tue Aug 30 00:00:00 EDT 2022

Krüppel-like factor 5 accelerates the pathogenesis of Alzheimer's disease via BACE1-mediated APP processing.

Alzheimer's research & therapy

Wang Y,Cui Y,Liu J,Song Q,Cao M,Hou Y,Zhang X,Wang P

Tue Jul 26 00:00:00 EDT 2022

Krüppel-like factor 5 accelerates the pathogenesis of Alzheimer's disease via BACE1-mediated APP processing.

Alzheimer's research & therapy

Wang Y,Cui Y,Liu J,Song Q,Cao M,Hou Y,Zhang X,Wang P

Tue Jul 26 00:00:00 EDT 2022

Krüppel-like factor 5 accelerates the pathogenesis of Alzheimer's disease via BACE1-mediated APP processing.

Alzheimer's research & therapy

Wang Y,Cui Y,Liu J,Song Q,Cao M,Hou Y,Zhang X,Wang P

Tue Jul 26 00:00:00 EDT 2022

Krüppel-like factor 5 accelerates the pathogenesis of Alzheimer's disease via BACE1-mediated APP processing.

Alzheimer's research & therapy

Wang Y,Cui Y,Liu J,Song Q,Cao M,Hou Y,Zhang X,Wang P

Tue Jul 26 00:00:00 EDT 2022

The protective effects of butorphanol tartrate against homocysteine-induced blood-brain barrier dysfunction.

Bioengineered

Shen S,Wang J,Zhao Q,Hu Q

Tue Mar 01 00:00:00 EST 2022

KLF5 promotes KIF1A expression through transcriptional repression of microRNA-338 in the development of pediatric neuroblastoma.

Journal of pediatric surgery

Zhou Y,Tang X,Huang Z,Wen J,Xiang Q,Liu D

Thu Sep 01 00:00:00 EDT 2022

The Mediator subunit MED20 organizes the early adipogenic complex to promote development of adipose tissues and diet-induced obesity.

Cell reports

Tang WS,Weng L,Wang X,Liu CQ,Hu GS,Yin ST,Tao Y,Hong NN,Guo H,Liu W,Wang HR,Zhao TJ

Tue Jul 06 00:00:00 EDT 2021

Evaluation of Barrier Functions in Human iPSC-Derived Intestinal Epithelium.

Methods in molecular biology (Clifton, N.J.)

Yamada S,Kanda Y

Thu Jan 13 00:00:00 EST 2022

Klf5 establishes bi-potential cell fate by dual regulation of ICM and TE specification genes.

Cell reports

Kinisu M,Choi YJ,Cattoglio C,Liu K,Roux de Bezieux H,Valbuena R,Pum N,Dudoit S,Huang H,Xuan Z,Kim SY,He L

Tue Nov 09 00:00:00 EST 2021

Autophagy-induced p62 accumulation is required for curcumol to regulate KLF5-mediated angiogenesis in liver sinusoidal endothelial cells.

Toxicology

Gao L,Yang X,Liang B,Jia Y,Tan S,Chen A,Cao P,Zhang Z,Zheng S,Sun L,Zhang F,Shao J

Tue Mar 30 00:00:00 EDT 2021

Autophagy-induced p62 accumulation is required for curcumol to regulate KLF5-mediated angiogenesis in liver sinusoidal endothelial cells.

Toxicology

Gao L,Yang X,Liang B,Jia Y,Tan S,Chen A,Cao P,Zhang Z,Zheng S,Sun L,Zhang F,Shao J

Tue Mar 30 00:00:00 EDT 2021

Autophagy-induced p62 accumulation is required for curcumol to regulate KLF5-mediated angiogenesis in liver sinusoidal endothelial cells.

Toxicology

Gao L,Yang X,Liang B,Jia Y,Tan S,Chen A,Cao P,Zhang Z,Zheng S,Sun L,Zhang F,Shao J

Tue Mar 30 00:00:00 EDT 2021

Autophagy-induced p62 accumulation is required for curcumol to regulate KLF5-mediated angiogenesis in liver sinusoidal endothelial cells.

Toxicology

Gao L,Yang X,Liang B,Jia Y,Tan S,Chen A,Cao P,Zhang Z,Zheng S,Sun L,Zhang F,Shao J

Tue Mar 30 00:00:00 EDT 2021

Identification and Characterization of TF-lncRNA Regulatory Networks Involved in the Tumorigenesis and Development of Adamantinomatous Craniopharyngioma.

Frontiers in oncology

Xu D,Guo Y,Lei S,Guo A,Song D,Gao Q,Zhao S,Yin K,Wei Q,Zhang L,Wang X,Wang J,Zhang Q,Guo F

Wed Feb 16 00:00:00 EST 2022

A Weighted Gene Co-Expression Network Analysis-Derived Prognostic Model for Predicting Prognosis and Immune Infiltration in Gastric Cancer.

Frontiers in oncology

Chen Q,Tan Y,Zhang C,Zhang Z,Pan S,An W,Xu H

Thu Apr 21 00:00:00 EDT 2022

Curcumol inhibits KLF5-dependent angiogenesis by blocking the ROS/ERK signaling in liver sinusoidal endothelial cells.

Life sciences

Gao L,Yang X,Li Y,Wang Z,Wang S,Tan S,Chen A,Cao P,Shao J,Zhang Z,Zhang F,Zheng S

Fri Jan 01 00:00:00 EST 2021

Curcumol inhibits KLF5-dependent angiogenesis by blocking the ROS/ERK signaling in liver sinusoidal endothelial cells.

Life sciences

Gao L,Yang X,Li Y,Wang Z,Wang S,Tan S,Chen A,Cao P,Shao J,Zhang Z,Zhang F,Zheng S

Fri Jan 01 00:00:00 EST 2021

An inhibitor of Krüppel-like factor 5 suppresses peritoneal fibrosis in mice.

Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis

Muta K,Nakazawa Y,Obata Y,Inoue H,Torigoe K,Nakazawa M,Abe K,Furusu A,Miyazaki M,Yamamoto K,Koji T,Nishino T

Thu Jul 01 00:00:00 EDT 2021

CircATRNL1 protects against osteoarthritis by targeting miR-153-3p and KLF5.

International immunopharmacology

Wang KF,Shi ZW,Dong DM

Thu Jul 01 00:00:00 EDT 2021

Transcription factor Kruppel-like factor 5 positively regulates the expression of AarF domain containing kinase 4.

Molecular biology reports

Chen X,Liu S,Chen J,Wang X,Zhou G

Sun Nov 01 00:00:00 EDT 2020

Transcription factor Kruppel-like factor 5 positively regulates the expression of AarF domain containing kinase 4.

Molecular biology reports

Chen X,Liu S,Chen J,Wang X,Zhou G

Sun Nov 01 00:00:00 EDT 2020

KLF5 Is Crucial for Androgen-AR Signaling to Transactivate Genes and Promote Cell Proliferation in Prostate Cancer Cells.

Cancers

Li J,Zhang B,Liu M,Fu X,Ci X,A J,Fu C,Dong G,Wu R,Zhang Z,Fu L,Dong JT

Sat Mar 21 00:00:00 EDT 2020

KLF5 Is Crucial for Androgen-AR Signaling to Transactivate Genes and Promote Cell Proliferation in Prostate Cancer Cells.

Cancers

Li J,Zhang B,Liu M,Fu X,Ci X,A J,Fu C,Dong G,Wu R,Zhang Z,Fu L,Dong JT

Sat Mar 21 00:00:00 EDT 2020

mircroRNA-152 prevents the malignant progression of atherosclerosis via down-regulation of KLF5.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Wang W,Zhang Y,Wang L,Li J,Li Y,Yang X,Wu Y

Tue Jan 01 00:00:00 EST 2019

mircroRNA-152 prevents the malignant progression of atherosclerosis via down-regulation of KLF5.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Wang W,Zhang Y,Wang L,Li J,Li Y,Yang X,Wu Y

Tue Jan 01 00:00:00 EST 2019

The long non-coding RNA TUG1-miR-9a-5p axis contributes to ischemic injuries by promoting cardiomyocyte apoptosis via targeting KLF5.

Cell death & disease

Yang D,Yu J,Liu HB,Yan XQ,Hu J,Yu Y,Guo J,Yuan Y,Du ZM

Mon Dec 02 00:00:00 EST 2019

Retinoic acid promotes barrier functions in human iPSC-derived intestinal epithelial monolayers.

Journal of pharmacological sciences

Yamada S,Kanda Y

Thu Aug 01 00:00:00 EDT 2019

Overexpression of KLF5 is associated with poor survival and G1/S progression in pancreatic cancer.

Aging

Li Y,Kong R,Chen H,Zhao Z,Li L,Li J,Hu J,Zhang G,Pan S,Wang Y,Wang G,Chen H,Sun B

Sun Jul 21 00:00:00 EDT 2019

Overexpression of KLF5 is associated with poor survival and G1/S progression in pancreatic cancer.

Aging

Li Y,Kong R,Chen H,Zhao Z,Li L,Li J,Hu J,Zhang G,Pan S,Wang Y,Wang G,Chen H,Sun B

Sun Jul 21 00:00:00 EDT 2019

miR-20a-5p promotes adipogenic differentiation of murine bone marrow stromal cells via targeting Kruppel-like factor 3.

Journal of molecular endocrinology

Zhu E,Zhang J,Zhou J,Yuan H,Zhao W,Wang B

Sun Apr 01 00:00:00 EDT 2018

miR-20a-5p promotes adipogenic differentiation of murine bone marrow stromal cells via targeting Kruppel-like factor 3.

Journal of molecular endocrinology

Zhu E,Zhang J,Zhou J,Yuan H,Zhao W,Wang B

Sun Apr 01 00:00:00 EDT 2018

KLF5 promotes the tumorigenesis and metastatic potential of thyroid cancer cells through the NF-κB signaling pathway.

Oncology reports