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KLF5 Polyclonal Antibody

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KLF5 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 21017-1-AP (KLF5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

KLF5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse embryo tissue slide using 21017-1-AP ( KLF5 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

KLF5 Antibody in Western Blot (WB) — Mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

KLF5 Antibody in Immunoprecipitation (IP) — IP result of anti-KLF5 (IP:21017-1-AP, 4ug; Detection:21017-1-AP 1:500) with HeLa cells lysate 2800ug.

Product Details

21017-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 6 publications 6 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 1 publication 1 publication
Immunoprecipitation (IP)	0.5-4.0 µg	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KLF5 Fusion Protein Ag15267 (1-350 aa encoded by BC042131) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.4 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody detects the 50-60 kDa isoform 1 of KLF5. The antibody can recognize isoform 2 (33 kDa) and isoform 3 (18 kDa) too.

Immunogen sequence: MATRVLSMS ARLGPVPQPP APQDEPVFAQ LKPVLGAANP ARDAALFPGE ELKHAHHRPQ AQPAPAQAPQ PAQPPATGPR LPPEDLVQTR CEMEKYLTPQ LPPVPIIPEH KKYRRDSASV VDQFFTDTEG LPYSINMNVF LPDITHLRTG LYKSQRPCVT HIKTEPVAIF SHQSETTAPP PAPTQALPEF TSIFSSHQTA APEVNNIFIK QELPTPDLHL SVPTQQGHLY QLLNTPDLDM PSSTNQTAAM DTLNVSMSAA MAGLNTHTSA VPQTAVKQFQ GMPPCTYTMP SQFLPQQATY FPPSPPSSEP GSPDRQAEML QNLTPPPSYA ATIASKLAIH NPNLPTTLPV N (1-350 aa encoded by BC042131)

Target Information

KLF5 is a member of the Kruppel-like factor subfamily of zinc finger proteins. It contains three C2H2-type zinc fingers at the carboxyl-terminus that preferentially bind to cis-DNA elements that are GC rich. KLF5 is a transcription activator that promotes cell proliferation and oncogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

mircroRNA-152 prevents the malignant progression of atherosclerosis via down-regulation of KLF5.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Wang W,Zhang Y,Wang L,Li J,Li Y,Yang X,Wu Y

Tue Jan 01 00:00:00 EST 2019

miR-448-3p controls intracranial aneurysm by regulating KLF5 expression.

Biochemical and biophysical research communications

Zhang JZ,Chen D,Lv LQ,Xu Z,Li YM,Wang JY,Han KW,Yu MK,Huang CG,Hou LJ

Sat Nov 10 00:00:00 EST 2018

miR-448-3p controls intracranial aneurysm by regulating KLF5 expression.

Biochemical and biophysical research communications

Zhang JZ,Chen D,Lv LQ,Xu Z,Li YM,Wang JY,Han KW,Yu MK,Huang CG,Hou LJ

Sat Nov 10 00:00:00 EST 2018

KLF5 promotes the tumorigenesis and metastatic potential of thyroid cancer cells through the NF-κB signaling pathway.

Oncology reports

Ma Y,Wang Q,Liu F,Ma X,Wu L,Guo F,Zhao S,Huang F,Qin G

Thu Nov 01 00:00:00 EDT 2018

KLF5 promotes the tumorigenesis and metastatic potential of thyroid cancer cells through the NF-κB signaling pathway.

Oncology reports

Ma Y,Wang Q,Liu F,Ma X,Wu L,Guo F,Zhao S,Huang F,Qin G

Thu Nov 01 00:00:00 EDT 2018

KLF5 promotes the tumorigenesis and metastatic potential of thyroid cancer cells through the NF-κB signaling pathway.

Oncology reports

Ma Y,Wang Q,Liu F,Ma X,Wu L,Guo F,Zhao S,Huang F,Qin G

Thu Nov 01 00:00:00 EDT 2018

Regulation of the adaptation to ER stress by KLF4 facilitates melanoma cell metastasis via upregulating NUCB2 expression.

Journal of experimental & clinical cancer research : CR

Zhang D,Lin J,Chao Y,Zhang L,Jin L,Li N,He R,Ma B,Zhao W,Han C

Sat Jul 28 00:00:00 EDT 2018

miR-20a-5p promotes adipogenic differentiation of murine bone marrow stromal cells via targeting Kruppel-like factor 3.

Journal of molecular endocrinology

Zhu E,Zhang J,Zhou J,Yuan H,Zhao W,Wang B

Sun Apr 01 00:00:00 EDT 2018

Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells.

Oncotarget

Wei X,Ye J,Shang Y,Chen H,Liu S,Liu L,Wang R

Tue Dec 12 00:00:00 EST 2017

DNMT1-maintained hypermethylation of Krüppel-like factor 5 involves in the progression of clear cell renal cell carcinoma.

Cell death & disease

Fu RJ,He W,Wang XB,Li L,Zhao HB,Liu XY,Pang Z,Chen GQ,Huang L,Zhao KW

Thu Jul 27 00:00:00 EDT 2017

ERK kinase phosphorylates and destabilizes the tumor suppressor FBW7 in pancreatic cancer.

Cell research

Ji S,Qin Y,Shi S,Liu X,Hu H,Zhou H,Gao J,Zhang B,Xu W,Liu J,Liang D,Liu L,Liu C,Long J,Zhou H,Chiao PJ,Xu J,Ni Q,Gao D,Yu X

Fri May 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: (intestinal Kruppel-like factor; basic transcription element binding protein 2; basic transcription element binding protein BTEB2; Basic transcription element-binding protein 2; BTE-binding protein 2; Colon krueppel-like factor; colon kruppel-like factor; GC box binding protein 2; GC-box-binding protein 2; Intestinal-enriched krueppel-like factor; intestinal-enriched kruppel-like factor; Klf5C isoform; Krueppel-like factor 5; Kruppel-like factor 5 (intestinal); RP11-505F3.5; Transcription factor BTEB2

Gene Aliases: 4930520J07Rik; BTEB2; CKLF; IKLF; KLF5

UniProt ID: (Human) Q13887, (Mouse) Q9JMI2

Entrez Gene ID: (Human) 688, (Rat) 84410, (Mouse) 12224

Molecular Function: C2H2 zinc finger transcription factor