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Invitrogen
KLRB1 Polyclonal Antibody
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FIGURE: 1 / 1
KLRB1 Antibody (PA5-88255) in WB
Western blot analysis of KLRB1 in extracts of various cell lines. Samples were incubated with KLRB1 Polyclonal antibody (Product # PA5-88255) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-88255
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 73-140 of human KLRB1 (NP_0022491)
Conjugate
Form
Liquid
Concentration
0.73 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: KCSVDIQQSR NKTTERPGLL NCPIYWQQLR EKCLLFSHTV NPWNNSLADC STKESSLLLI RDKDELIH; Positive Samples: THP-1, Mouse liver; Cellular Location: Membrane, Single-pass type II membrane protein
Target Information
KLRB1 (Killer cell lectin like receptor subfamily B, member 1, CD-161) protein is classified as a type II membrane protein because it has an external C terminus. KLRB1 cell surface antigen is expressed by almost all NK cells and in a small subset of CD3+ve T cells. KLRB1 is a homodimeric cell surface protein, comprising two chains with molecular weights ranging from 40-44kDa. KLRB1 plays an inhibitory role on natural killer (NK) cells. Activation of KLRB1 leads to acid sphingomyelnase/SMPD1 stimulation and an increase in intracellular ceramide. Moreover, there is also an activation of AKT1/PKB and RPS6KA1/RSK1 kinase stimulation, and T cell proliferation by anti-CD3. KLRB1 acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope and to the N-acetyllactosamine epitope. KLRB1 also binds to CLEC2D/LLT1 as a ligand, and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-type lectin domain family 5 member B; CD161; CD161 antigen-like family member A; CD161 antigen-like family member B; CD161a; CD161b; HNKR-P1a; immunoreceptor NKR-P1G; Inhibitory receptor NKR-P1B; killer cell lectin-like receptor subfamily A member 1 complex; Killer cell lectin-like receptor subfamily B member 1; killer cell lectin-like receptor subfamily B member 1 complex; Killer cell lectin-like receptor subfamily B member 1A; killer cell lectin-like receptor subfamily B member 1B allele A; Killer cell lectin-like receptor subfamily B member 1B allele B; killer cell lectin-like receptor subfamily B member 1D; Killer cell lectin-like receptor subfamily B member 1G; killer cell lectin-like receptor subfamily B, member 1; lectin-like transmembrane protein Nkrp1g; Ly-55A; ly-55b; Ly-55d; lymphocyte antigen 55; lymphocyte antigen 55 complex, locus A; lymphocyte antigen 55 complex, locus B; lymphocyte antigen 55 complex, locus D; Lymphocyte antigen 55A; lymphocyte antigen 55b; Lymphocyte antigen 55d; MGC138614; natural killer cell receptor; natural killer cell receptor protein NKR-P1A; natural killer cell receptor protein NKR-P1B; natural killer cell receptor-P1; Natural killer cell surface protein NKR-P1B allele B6; natural killer cell surface protein NKR-P1B allele SJL/BALB; Natural killer cell surface protein NKR-P1G; Natural killer cell surface protein P1-2; Natural killer cell surface protein P1A; Natural killer lectin-like receptor 1E; natural killer lectin-like receptor protein 1 e; NKR-P1 2; NKR-P1 34; NKR-P1.7; NKR-P1A; NKR-P1D
Gene Aliases: 4930431A04Rik; CD161; CLEC5B; Gm4696; hNKR-P1A; KLRB1; Klrb1a; Klrb1b; Klrb1d; Klrb1g; Klrb6; Ly-55; ly-55A; Ly55; Ly55a; Ly55b; Ly55d; NKR; NKR-P1; NKR-P1.7; NKR-P1A; NKR-P1B; NKR-P1D; NKR-P1G; Nkrp-1e; Nkrp1-a; Nkrp1-b; NKRP12; NKRP1A; Nkrp1b; Nkrp1d; Nkrp1g
UniProt ID: (Human) Q12918, (Mouse) Q0ZUP1, (Mouse) P27811, (Mouse) Q99JB4
Entrez Gene ID: (Human) 3820, (Mouse) 100043861, (Mouse) 17057, (Mouse) 80782
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