A suggested positive control is Hela cell lysate.
PA5-21032 can be used with blocking peptide PEP-1146.
Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA1, also known as importin alpha 5, is the alpha subunit of karyopherin, which forms a complex with importin subunit beta-1 and functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. It is ubiquitously expressed and polyubiquitinated in the presence of RAG1. KPNA1 interacts with various virus nucleoproteins, including those of Ebola and influenza.
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Protein Aliases: importin alpha 5; importin alpha-5; Importin alpha-S1; importin subunit alpha-1; Importin subunit alpha-5; Importin subunit alpha-5, N-terminally processed; importin-alpha-S1; IPOA5, NPI-1, RCH2, SRP1; karyopherin (importin) alpha 1; karyopherin alpha 1 (importin alpha 5); Karyopherin subunit alpha-1; karyopherin/importin alpha-1; m-importin-alpha-S1; NPI-1; Nucleoprotein interactor 1; RAG cohort protein 2; recombination activating gene cohort 2; SRP1-beta
Gene Aliases: AW494490; IPOA5; KPNA1; mSRP1; NPI-1; NPI1; RCH2; SRP1
Molecular Function: transfer/carrier protein