Immunofluorescent analysis of KPNA2 in 4% formaldahyde-fixed HeLa cells using a KPNA2 polyclonal antibody (Product # PA1-32094) (top panel and green sections of bottom panel) and endogenously expressed Histone H2B-GFP fusion as DNA marker (mid panel and red sections of bottom panel).
|Tested species reactivity||Dog, Human, Mouse, Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence QVQDGAPGTFNF, from C Terminus of the protein sequence according to NP_002257|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-32094 detects KPNA2 from human samples.
PA1-32094 has been successfully used in Western blot applications.
The PA1-32094 immunogen is: Peptide with sequence QVQDGAPGTFNF, from C Terminus of the protein sequence according to NP_002257
Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (N
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