Immunohistochemical analysis of KPNA4 in paraffin-embedded human small intestine tissue using a KPNA4 polyclonal antibody (Product # PA1-32097) at a dilution of 5 µg/ml. Steamed antigen retrieval was performed using a citrate buffer, pH 6, followed by AP-staining.
|Tested species reactivity||Bovine, Dog, Human, Mouse, Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-NSSANVPTEGFQF.|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||0.2-1 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-32097 detects KPNA4 from human, mouse, rat, dog, and cow samples.
PA1-32097 has been successfully used in Western blot applications.
The PA1-32097 immunogen is a peptide with sequence C-NSSANVPTEGFQF.
The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.