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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Cytokeratin 12 Antibodies

          Invitrogen

          KRT12 Polyclonal Antibody

          View all (11) Cytokeratin 12 antibodies

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          Cite KRT12 Polyclonal Antibody

          • Antibody Testing Data (1)
          KRT12 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          KRT12 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          KRT12 Antibody (PA5-109895) in WB

          Western blot analysis of KRT12 in extracts of various cell lines. Samples were incubated with KRT12 Polyclonal antibody (Product # PA5-109895) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          KRT12 Antibody in Western Blot (WB)
          KRT12 Polyclonal Antibody

          Product Details

          PA5-109895

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant fusion protein containing a sequence corresponding to amino acids 295-494 of human Cytokeratin 12 (Cytokeratin 12 (KRT12)) (NP_0002141)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.78 mg/mL

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2855306

          Product Specific Information

          Immunogen sequence: TRLLNDMRAQ YETIAEQNRK DAEAWFIEKS GELRKEISTN TEQLQSSKSE VTDLRRAFQN LEIELQSQLA MKKSLEDSLA EAEGDYCAQL SQVQQLISNL EAQLLQVRAD AERQNVDHQR LLNVKARLEL EIETYRRLLD GEAQGDGLEE SLFVTDSKSQ AQSTDSSKDP TKTRKIKTVV QEMVNGEVVS SQVQEIEELM

          Target Information

          KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: CK-12; cytokeratin-12; K12; keratin 12 (Meesmann corneal dystrophy); keratin complex 1, acidic, gene 12; keratin K12; keratin, type I cytoskeletal 12; Keratin, type I cytoskeletal 12 (Cytokeratin 12); keratin-12; krt12 {ECO:0000250|UniProtKB:Q99456}; type I keratin KA12

          View more View less

          Gene Aliases: AI835216; K12; Ka12; Krt-12; Krt1-12

          View more View less

          Entrez Gene ID: (Mouse) 268482, (Rat) 360625

          View more View less

          Function(s)
          structural molecule activity molecular_function
          Process(es)
          epithelium development cornea development in camera-type eye biological_process
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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