Immunofluorescent analysis of KRT71 showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a KRT71 polyclonal antibody (Product # PA5-28558) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 211 and 523 of Human KRT71|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28558 targets KRT71 in WB applications and shows reactivity with Human samples.
The PA5-28558 immunogen is recombinant fragment corresponding to a region within amino acids 211 and 523 of Human KRT71.
K6IRS1 belongs to a family of type II keratins that are specifically expressed in the inner root sheath of hair follicles.
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