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Agrisera
Kelch repeat protein Polyclonal Antibody
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FIGURE: 1 / 1
Kelch repeat protein Antibody (AS14-2761) in WB
Total protein from Chlamydomonas reinhardtii (1), Gonium Pectorale (2), Eudorina elegans (3) was extracted using 1 x LaemmLi buffer + DTT + protease and phosphatase inhibitors without bromophenol blue heated at 95 ° C for ten minutes then vortexed with zirconia beads at max speed for ten minutes. Lysate was spun at 4 ° C for ten minutes at max speed in a tabletop centrifuge. Proteins were precipitated out o... View More
Product Details
AS14-2761
Product Specifications
Species Reactivity
Algae
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Cr-Klech.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Chlamydomonas reinhardtii (strain 21grm cw15, M10), Gonium pectorale, Eudorina elegans
Target Information
Kelch repeat proteins of the KLHL family are intracellular, predominantly cytoplasmic adaptors that concentrate on specific subcellular structures such as the actin cytoskeleton and, for some members, early secretory pathway membranes (endoplasmic reticulum exit sites/COPII assemblies). Structurally, they are modular proteins built around an N-terminal BTB/POZ domain (supporting homodimerization and Cullin 3 engagement), a central BACK domain that contributes to Cullin 3 complex assembly, and a C-terminal Kelch-repeat beta-propeller that provides the principal substrate-recognition interface. Functionally, KLHL proteins act as substrate receptors for Cullin 3-RING E3 ubiquitin ligases (CRL3), thereby specifying ubiquitination (often degradative, but also regulatory mono-ubiquitination) of selected client proteins to control protein turnover and pathway output; for example, KLHL12 recruits SEC31 for mono-ubiquitination to promote formation of enlarged COPII coats required for efficient export of bulky procollagen and collagen secretion, linking kelch-repeat adaptors to extracellular matrix biogenesis and secretory capacity, while other kelch-repeat adaptors couple cytoskeletal organization to ubiquitin-dependent remodeling programs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: F6E13.26; KISS ME DEADLY 3; KMD3
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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