Invitrogen

Ki-67 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

37 Published Figures

53 References

View all (153) Ki-67 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Ki-67 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Ki67 was performed using 70% confluent log phase HeLa cells serum starved for 36 Hrs followed by serum release for 6 Hrs. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Ki-67 Polyclonal Antibody (Product # PA5-19462) at 1 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents serum starved cells with reduced signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Ki-67 Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis using Anti-Ki-67 Polyclonal Antibody (Product # PA5-19462) shows increased expression of proteins upon serum starvation followed by serum release in HeLa cells. {TM}

Ki-67 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Figure 5 Intraventricular injection of MSC-Exo alleviates SE-induced hippocampal astrocytic activation in mice. The influence of MSC-Exo on hippocampal astrocytic activation was examined in the acute and chronic stages of SE. (A-L) Representative images display the immunofluorescence intensity of GFAP (A-L, red), CD81 (A-D, green), C3 (E-H, green), and ki67 (I-L, green) in the hippocampus at 4 d post-SE, cell nuclei stained with DAPI (blue). (M-P) Fluorescence intensity assay of the relative expression of GFAP (M), CD81 (N), C3 (O), and ki67 (P) in each group. (Q) Western blots of protein expression of C3, CD81, and GFAP at 24 h, 4 d, 7 d, and 8 w post-SE in the hippocampus. (R-T) Statistical analysis of changes in C3 (R), CD81 (S), and GFAP (T) expression in each group. Statistical difference between groups is displayed in histograms. Scale bars: A-L, 50 mum; A1-L1 and A2-L2, 20 mum.

Ki-67 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunohistochemistry (Paraffin)

Ki-67 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P)) — Figure 5 Tumor growth in PyMT/Ppp1r1b +/+ and PyMT/Ppp1r1b -/- mice. (A) The age at which tumors first appeared (tumor volume 100-300 mm 3 ) was recorded for wild-type (PyMT/Ppp1r1b +/+ ) and knockout (PyMT/Ppp1r1b -/- ) tumor mice (n = 14 per group). Individual ages and the mean age at appearance (+-standard error of the mean) are shown. (B) Kaplan-Meier survival plot showing the age at which mice were euthanized because of extensive tumor burden (maximum tumor volume >1500 mm 3 ). (C-D) Mice surviving to 20 weeks of age were euthanized and tumor volume was measured. (C) Total tumor volume in these mice was calculated as the sum of the individual tumor volumes for each mouse. (D) Maximum tumor volume was defined as the volume of the largest individual tumor per mouse ( n = 8-12 per group); mean +- standard error of the mean, * p < 0.05. (E-F) Tumors were collected at 20 weeks of age, formalin-fixed, sectioned and immuno-stained for Ki67 and CD31. (E) Representative tumor sections and bar graph showing the mean (+- standard error of the mean) number of Ki67-positive cells per field in 10 nonoverlapping fields from three tumors per group (25x magnification, scale bar = 50 mum). (F) Representative tumor sections and bar graph showing the mean (+- standard error of the mean) microvessel density determined by counting the number of CD31-positive endothelial clusters (white arrows) in 10 nonoverlapping fields from three tumors per group (25x magnification, scale bar = 50 mum). (G)

Ki-67 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Fig. 4. Reduction of GABPB1L potentiates anti-tumor response of TERT p mutant GBM to chemotherapy. ( A ) A schematic for possible sensitization of TERT promoter mutant GBM cells to TMZ through GABPB1L inhibition. Inhibiting GABPB1L leads to reduced TERT expression, resulting in a blunted DDR and lack of normal cell cycle arrest, ultimately reducing cancer cell viability following DNA damage. SSB, single-strand break; DSB, double-strand break; DDR, DNA-damage response. ( B ) Representative immunoblots of yH2AX in U-251 cells expressing doxycycline-induced shRNAs targeting TERT (shTERT.3795 and shTERT.3592) or GABPB1L (shGB1L.968 and shGB1L.1202) compared with a nontargeting (renilla luciferase, shRen.713) shRNA. ( C ) Representative immunoblots ( Bottom ) and quantification ( Top , from triplicate blots) of yH2AX in U-251 cells engineered to stably express either BFP (control) or TERT (rescue) in the presence of shRNAs targeting TERT (shTERT.3795 and shTERT.3592) or GABPB1L (shGB1L.968 and shGB1L.1202) compared with a nontargeting shRNA (renilla luciferase, shRen.713). ( B and C ) Cells were incubated with doxycycline for 6 d prior to harvest and treated with specified dose(s) of TMZ 20 h prior to harvest. ( D ) Representative images ( Left ) and G2/G1 ratio quantification ( Right ) from flow cytometry-based cell cycle analysis of U-251 WT and GABPB1L heterozygous knockout cells treated with a dose titration of TMZ 72 h prior to harvest. p, P value (unpaired two-tailed Student

Ki-67 Antibody in Immunohistochemistry (IHC) — Fig. 1 The role of KDM6A in proliferation of BCa cells. a The protein expression levels of KDM6A in the BCa cells were determined by Western blot. b The lentivirus mediated KDM6A overexpression in BCa cells were examined by Western blot. c and d Effect of KDM6A overexpression on cell proliferation was examined by MTT assays (c) and colony formation assays (d) in the BCa cells. e The protein expression levels of KDM6A in the indicated cells were determined by Western blot. f The image of subcutaneous tumours developed by the indicated T24 cells peeled from mice was shown. g The representative IHC images of KDM6A and Ki67 in subcutaneous tumours were shown. Scale bars, 50 mum (left) and 20 mum (right). h Cell migration assay of bone marrow monocyte derived-macrophages cocultured with conditioned medium (CM) from indicated cells. All quantification analyses were based on independent triplicate experiments. Error bars represent SD. ** p < 0.01, NS no significant, based on Student's t test

Product Details

PA5-19462

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 36 publications 36 publications
Immunohistochemistry (Paraffin) (IHC (P))	0.1 - 5 µg/mL	View 4 publications 4 publications
Immunohistochemistry (Frozen) (IHC (F))	-	View 3 publications 3 publications
Immunocytochemistry (ICC/IF)	0.5-1 µg/ml	View 6 publications 6 publications
Miscellaneous PubMed (Misc)	-	View 4 publications 4 publications

Product Specifications
Species Reactivity	Bovine, Dog, Horse, Hamster, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat
Published species	Human, Mouse, Rat, Sheep
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide conjugated to KLH derived from within residues 1200 - 1300 of Human Ki67. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_10981523

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to tissue staining.

Recommended positive controls:

ICC/IF - Wildtype HAP1 cells, SK-N-SH cells, HeLa cells, MEF1 cells

IHC (P) - Mouse and human spleen tissue, human skin carcinoma tissue, human colon tissue, mouse tumor tissue, human skin tissue

Target Information

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Differential Proliferation and Maturation of Subcortical Astrocytes During Postnatal Development.

Frontiers in neuroscience

Shoneye T,Orrego AT,Jarvis R,Men Y,Chiang MSR,Yang Y

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunocytochemistry

Sat Apr 16 00:00:00 EDT 2022

Differential Proliferation and Maturation of Subcortical Astrocytes During Postnatal Development.

Frontiers in neuroscience

Shoneye T,Orrego AT,Jarvis R,Men Y,Chiang MSR,Yang Y

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunocytochemistry

Sat Apr 16 00:00:00 EDT 2022

LncRNA MNX1-AS1 Contributes to Laryngeal Squamous Cell Carcinoma Growth and Migration by Regulating mir-744-5p/bcl9/β-Catenin Axis.

Cell transplantation

Ma B,Ren G,Xu J,Yin C,Shi Y

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunohistochemistry

Wed Nov 17 00:00:00 EST 2021

Canonical NF-κB signaling maintains corneal epithelial integrity and prevents corneal aging via retinoic acid.

eLife

Yu Q,Biswas S,Ma G,Zhao P,Li B,Li J

PA5-19462 was used in Immunohistochemistry to uncover the major mechanisms governing corneal maintenance, regeneration, and aging and identifies the NF-κB-retinoic acid pathway as a therapeutic target for corneal disorders.

Fri Jun 04 00:00:00 EDT 2021

Mutual dependency between lncRNA LETN and protein NPM1 in controlling the nucleolar structure and functions sustaining cell proliferation.

Cell research

Wang X,Hu X,Song W,Xu H,Xiao Z,Huang R,Bai Q,Zhang F,Chen Y,Liu Y,Fang J,Li X,Shen Q,Zhao H,Yang X

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunocytochemistry

Tue Jun 01 00:00:00 EDT 2021

Mutual dependency between lncRNA LETN and protein NPM1 in controlling the nucleolar structure and functions sustaining cell proliferation.

Cell research

Wang X,Hu X,Song W,Xu H,Xiao Z,Huang R,Bai Q,Zhang F,Chen Y,Liu Y,Fang J,Li X,Shen Q,Zhao H,Yang X

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunocytochemistry

Tue Jun 01 00:00:00 EDT 2021

Retinoic Acid Is Required for Oligodendrocyte Precursor Cell Production and Differentiation in the Postnatal Mouse Corpus Callosum.

eNeuro

Morrison VE,Smith VN,Huang JK

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunofluorescence

Fri May 21 00:00:00 EDT 2021

KDM6A-ARHGDIB axis blocks metastasis of bladder cancer by inhibiting Rac1.

Molecular cancer

Liu L,Cui J,Zhao Y,Liu X,Chen L,Xia Y,Wang Y,Chen S,Sun S,Shi B,Zou Y

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunohistochemistry

Tue May 18 00:00:00 EDT 2021

KDM6A-ARHGDIB axis blocks metastasis of bladder cancer by inhibiting Rac1.

Molecular cancer

Liu L,Cui J,Zhao Y,Liu X,Chen L,Xia Y,Wang Y,Chen S,Sun S,Shi B,Zou Y

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunohistochemistry

Tue May 18 00:00:00 EDT 2021

Cancer-specific loss of TERT activation sensitizes glioblastoma to DNA damage.

Proceedings of the National Academy of Sciences of the United States of America

Amen AM,Fellmann C,Soczek KM,Ren SM,Lew RJ,Knott GJ,Park JE,McKinney AM,Mancini A,Doudna JA,Costello JF

PA5-19462 was used in Immunohistochemistry (Frozen) to provide insights into the mechanism of cancer-specific TERT regulation, uncover rapid effects of GABPB1L-mediated TERT suppression in GBM maintenance, and establish GABPB1L inhibition in combination with chemotherapy as a therapeutic strategy for TERT promoter mutant GBM.

Tue Mar 30 00:00:00 EDT 2021

Cancer-specific loss of TERT activation sensitizes glioblastoma to DNA damage.

Proceedings of the National Academy of Sciences of the United States of America

Amen AM,Fellmann C,Soczek KM,Ren SM,Lew RJ,Knott GJ,Park JE,McKinney AM,Mancini A,Doudna JA,Costello JF

Tue Mar 30 00:00:00 EDT 2021

Myocyte Enhancer Factor 2C as a New Player in Human Breast Cancer Brain Metastases.

Cells

Galego S,Kauppila LA,Malhó R,Pimentel J,Brito MA

PA5-19462 was used in Immunohistochemistry to ascertain MEF2C expression in human BCBM, establish the relationship with disease severity, disclose the involvement of vascular endothelial growth factor receptor-2 (VEGFR-2) and β-catenin, also known as KDR and CTNNB1, respectively, and investigate if matched primary tumors express the protein.

Fri Feb 12 00:00:00 EST 2021

Mesenchymal stem cell-derived exosomes as a nanotherapeutic agent for amelioration of inflammation-induced astrocyte alterations in mice.

Theranostics

Xian P,Hei Y,Wang R,Wang T,Yang J,Li J,Di Z,Liu Z,Baskys A,Liu W,Wu S,Long Q

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunofluorescence

Mon Aug 31 00:00:00 EDT 2020

Mesenchymal stem cell-derived exosomes as a nanotherapeutic agent for amelioration of inflammation-induced astrocyte alterations in mice.

Theranostics

Xian P,Hei Y,Wang R,Wang T,Yang J,Li J,Di Z,Liu Z,Baskys A,Liu W,Wu S,Long Q

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunofluorescence

Mon Aug 31 00:00:00 EDT 2020

Therapeutic paradigm of dual targeting VEGF and PDGF for effectively treating FGF-2 off-target tumors.

Nature communications

Hosaka K,Yang Y,Seki T,Du Q,Jing X,He X,Wu J,Zhang Y,Morikawa H,Nakamura M,Scherzer M,Sun X,Xu Y,Cheng T,Li X,Liu X,Li Q,Liu Y,Hong A,Chen Y,Cao Y

PA5-19462 was used in Immunohistochemistry to show that FGF-2 modulates tumor vessels by recruiting NG2+ pricytes onto tumor microvessels through a PDGFRβ-dependent mechanism.

Fri Jul 24 00:00:00 EDT 2020

RAGE impairs murine diabetic atherosclerosis regression and implicates IRF7 in macrophage inflammation and cholesterol metabolism.

JCI insight

Senatus L,López-Díez R,Egaña-Gorroño L,Liu J,Hu J,Daffu G,Li Q,Rahman K,Vengrenyuk Y,Barrett TJ,Dewan MZ,Guo L,Fuller D,Finn AV,Virmani R,Li H,Friedman RA,Fisher EA,Ramasamy R,Schmidt AM

PA5-19462 was used in Immunohistochemistry to add a new dimension to the immunometabolic sphere of perturbations that impair regression of established diabetic atherosclerosis.

Thu Jul 09 00:00:00 EDT 2020

Maternal arginine supplementation enhances thermogenesis in the newborn lamb.

Journal of animal science

McKnight SM,Simmons RM,Wu G,Satterfield MC

PA5-19462 was used in Immunohistochemistry to determine if maternal arginine supplementation could enhance thermogenesis in the neonate.

Fri May 01 00:00:00 EDT 2020

Plasminogen is a master regulator and a potential drug candidate for the healing of radiation wounds.

Cell death & disease

Fallah M,Viklund E,Bäckman A,Brodén J,Lundskog B,Johansson M,Blomquist M,Wilczynska M,Ny T

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunohistochemistry

Mon Mar 23 00:00:00 EDT 2020

β3-Adrenoreceptor Blockade Induces Stem Cells Differentiation in Melanoma Microenvironment.

International journal of molecular sciences

Calvani M,Bruno G,Dabraio A,Subbiani A,Bianchini F,Fontani F,Casazza G,Vignoli M,De Logu F,Frenos S,Filippi L,Favre C

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunohistochemistry

Thu Feb 20 00:00:00 EST 2020

Primary infection by Pneumocystis induces Notch-independent Clara cell mucin production in rat distal airways.

PloS one

Méndez A,Rojas DA,Ponce CA,Bustamante R,Beltrán CJ,Toledo J,García-Angulo VA,Henriquez M,Vargas SL

PA5-19462 was used in Immunohistochemistry-immunofluorescence to investigate the markers of Clara cell secretion and Notch1 activation in the lungs of immunocompetent rats at 40, 60, and 80 days of age during Pneumocystis primary infection with and without Valproic acid (VPA).

Tue Feb 11 00:00:00 EST 2020

NCAM regulates temporal specification of neural progenitor cells via profilin2 during corticogenesis.

The Journal of cell biology

Huang R,Yuan DJ,Li S,Liang XS,Gao Y,Lan XY,Qin HM,Ma YF,Xu GY,Schachner M,Sytnyk V,Boltze J,Ma QH,Li S

PA5-19462 was used in Immunohistochemistry-immunofluorescence to study the role of NCAM in mediating neural progenitor cell proliferation and fate decision during corticogenesis.

Mon Jan 06 00:00:00 EST 2020

Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice.

International journal of molecular sciences

Mensching L,Djogo N,Keller C,Rading S,Karsak M

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunofluorescence

Thu Aug 01 00:00:00 EDT 2019

Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice.

International journal of molecular sciences

Mensching L,Djogo N,Keller C,Rading S,Karsak M

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunofluorescence

Thu Aug 01 00:00:00 EDT 2019

Functional peroxisomes are required for β-cell integrity in mice.

Molecular metabolism

Baboota RK,Shinde AB,Lemaire K,Fransen M,Vinckier S,Van Veldhoven PP,Schuit F,Baes M

PA5-19462 was used in Immunohistochemistry to indicate that normal peroxisome metabolism in β-cells is crucial to preserve their structure and function.

Mon Apr 01 00:00:00 EDT 2019

FABP5 regulates the proliferation of clear cell renal cell carcinoma cells via the PI3K/AKT signaling pathway.

International journal of oncology

Lv Q,Wang G,Zhang Y,Han X,Li H,Le W,Zhang M,Ma C,Wang P,Ding Q

PA5-19462 was used in Immunohistochemistry to investigate the function of fatty acid binding protein 5 in clear cell renal cell carcinoma cells.

Mon Apr 01 00:00:00 EDT 2019

Host-associated niche metabolism controls enteric infection through fine-tuning the regulation of type 3 secretion.

Nature communications

Connolly JPR,Slater SL,O'Boyle N,Goldstone RJ,Crepin VF,Ruano-Gallego D,Herzyk P,Smith DGE,Douce GR,Frankel G,Roe AJ

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunofluorescence

Wed Oct 10 00:00:00 EDT 2018

Reduced levels of methyltransferase DNMT2 sensitize human fibroblasts to oxidative stress and DNA damage that is accompanied by changes in proliferation-related miRNA expression.

Redox biology

Lewinska A,Adamczyk-Grochala J,Kwasniewicz E,Deregowska A,Semik E,Zabek T,Wnuk M

PA5-19462 was used in Immunofluorescence to find that DNMT2 may take part in the regulation of cell proliferation and longevity in human fibroblasts and speculate that the manipulation of DNMT2 levels that limits cell proliferation may be potentially useful anticancer strategy.

Sun Apr 01 00:00:00 EDT 2018

Pericyte ALK5/TIMP3 Axis Contributes to Endothelial Morphogenesis in the Developing Brain.

Developmental cell

Dave JM,Mirabella T,Weatherbee SD,Greif DM

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunofluorescence

Mon Mar 26 00:00:00 EDT 2018

Periostin blockade overcomes chemoresistance via restricting the expansion of mesenchymal tumor subpopulations in breast cancer.

Scientific reports

Nakazawa Y,Taniyama Y,Sanada F,Morishita R,Nakamori S,Morimoto K,Yeung KT,Yang J

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunofluorescence

Mon Mar 05 00:00:00 EST 2018

Losmapimod Overcomes Gefitinib Resistance in Non-small Cell Lung Cancer by Preventing Tetraploidization.

EBioMedicine

Yeung YT,Yin S,Lu B,Fan S,Yang R,Bai R,Zhang C,Bode AM,Liu K,Dong Z

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunohistochemistry

Thu Feb 01 00:00:00 EST 2018

Autonomous and non-autonomous roles for ephrin-B in interneuron migration.

Developmental biology

Talebian A,Britton R,Ammanuel S,Bepari A,Sprouse F,Birnbaum SG,Szabó G,Tamamaki N,Gibson J,Henkemeyer M

PA5-19462 was used in Immunohistochemistry to define both receptor-like and ligand-like roles for the ephrin-B molecules in controlling the migration of interneurons as they populate the neocortex and help establish excitatory/inhibitory (E/I) homeostasis.

Wed Nov 15 00:00:00 EST 2017

Multifunctional (3-in-1) cancer theranostics applications of hydroxyquinoline-appended polyfluorene nanoparticles.

Chemical science

Chowdhury SR,Mukherjee S,Das S,Patra CR,Iyer PK

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunohistochemistry

Wed Nov 01 00:00:00 EDT 2017

Zika Virus Infects Intermediate Progenitor Cells and Post-mitotic Committed Neurons in Human Fetal Brain Tissues.

Scientific reports

Lin MY,Wang YL,Wu WL,Wolseley V,Tsai MT,Radic V,Thornton ME,Grubbs BH,Chow RH,Huang IC

PA5-19462 was used in Immunohistochemistry-immunofluorescence to study the effect of immune activation after zika virus infection on the pathogenesis of zika virus related microcephaly.

Wed Nov 01 00:00:00 EDT 2017

GD1a Overcomes Inhibition of Myelination by Fibronectin via Activation of Protein Kinase A: Implications for Multiple Sclerosis.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Qin J,Sikkema AH,van der Bij K,de Jonge JC,Klappe K,Nies V,Jonker JW,Kok JW,Hoekstra D,Baron W

PA5-19462 was used in Immunohistochemistry to propose that targeted delivery of GD1a to multiple sclerosis lesions may act as a potential novel molecular tool to boost maturation of resident OPCs to overcome remyelination failure and halt disease progression.

Wed Oct 11 00:00:00 EDT 2017

Personal samplers of bioavailable pesticides integrated with a hair follicle assay of DNA damage to assess environmental exposures and their associated risks in children.

Mutation research. Genetic toxicology and environmental mutagenesis

Vidi PA,Anderson KA,Chen H,Anderson R,Salvador-Moreno N,Mora DC,Poutasse C,Laurienti PJ,Daniel SS,Arcury TA

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunofluorescence

Sun Oct 01 00:00:00 EDT 2017

Personal samplers of bioavailable pesticides integrated with a hair follicle assay of DNA damage to assess environmental exposures and their associated risks in children.

Mutation research. Genetic toxicology and environmental mutagenesis

Vidi PA,Anderson KA,Chen H,Anderson R,Salvador-Moreno N,Mora DC,Poutasse C,Laurienti PJ,Daniel SS,Arcury TA

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunofluorescence

Sun Oct 01 00:00:00 EDT 2017

Novel role of NPY in neuroimmune interaction and lung growth after intrauterine growth restriction.

American journal of physiology. Lung cellular and molecular physiology

Thangaratnarajah C,Dinger K,Vohlen C,Klaudt C,Nawabi J,Lopez Garcia E,Kwapiszewska G,Dobner J,Nüsken KD,van Koningsbruggen-Rietschel S,von Hörsten S,Dötsch J,Alejandre Alcázar MA

PA5-19462 was used in Immunohistochemistry to provide new insights in pulmonary neuroimmune interaction, which offer potential strategies to enable lung growth.

Fri Sep 01 00:00:00 EDT 2017

Initiation of acute graft-versus-host disease by angiogenesis.

Blood

Riesner K,Shi Y,Jacobi A,Kräter M,Kalupa M,McGearey A,Mertlitz S,Cordes S,Schrezenmeier JF,Mengwasser J,Westphal S,Perez-Hernandez D,Schmitt C,Dittmar G,Guck J,Penack O

PA5-19462 was used in Immunohistochemistry-immunofluorescence to study the role of angiogenesis in the initiation of tissue inflammation during grant versus host disease.

Thu Apr 06 00:00:00 EDT 2017

Caspr Controls the Temporal Specification of Neural Progenitor Cells through Notch Signaling in the Developing Mouse Cerebral Cortex.

Cerebral cortex (New York, N.Y. : 1991)

Wu ZQ,Li D,Huang Y,Chen XP,Huang W,Liu CF,Zhao HQ,Xu RX,Cheng M,Schachner M,Ma QH

PA5-19462 was used in immunohistochemistry to report that Caspr regulates the temporal specification of cell fate in radial glial cells of the developing cerebral cortex through Notch signaling

Wed Feb 01 00:00:00 EST 2017

AKT1E¹⁷K Is Oncogenic in Mouse Lung and Cooperates with Chemical Carcinogens in Inducing Lung Cancer.

PloS one

Malanga D,Belmonte S,Colelli F,Scarfò M,De Marco C,Oliveira DM,Mirante T,Camastra C,Gagliardi M,Rizzuto A,Mignogna C,Paciello O,Papparella S,Fagman H,Viglietto G

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunohistochemistry

Mon Jul 18 00:00:00 EDT 2016

Conditional ablation of TGF-β signaling inhibits tumor progression and invasion in an induced mouse bladder cancer model.

Scientific reports

Liang Y,Zhu F,Zhang H,Chen D,Zhang X,Gao Q,Li Y

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunohistochemistry

Tue Jul 05 00:00:00 EDT 2016

Inhibition of Pediatric Glioblastoma Tumor Growth by the Anti-Cancer Agent OKN-007 in Orthotopic Mouse Xenografts.

PloS one

Coutinho de Souza P,Mallory S,Smith N,Saunders D,Li XN,McNall-Knapp RY,Fung KM,Towner RA

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunohistochemistry

Wed May 18 00:00:00 EDT 2016

Inhibition of Pediatric Glioblastoma Tumor Growth by the Anti-Cancer Agent OKN-007 in Orthotopic Mouse Xenografts.

PloS one

Coutinho de Souza P,Mallory S,Smith N,Saunders D,Li XN,McNall-Knapp RY,Fung KM,Towner RA

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunohistochemistry

Wed May 18 00:00:00 EDT 2016

High fat diet increases melanoma cell growth in the bone marrow by inducing osteopontin and interleukin 6.

Oncotarget

Chen GL,Luo Y,Eriksson D,Meng X,Qian C,Bäuerle T,Chen XX,Schett G,Bozec A

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunofluorescence

Tue May 03 00:00:00 EDT 2016

OKN-007 decreases tumor necrosis and tumor cell proliferation and increases apoptosis in a preclinical F98 rat glioma model.

Journal of magnetic resonance imaging : JMRI

de Souza PC,Balasubramanian K,Njoku C,Smith N,Gillespie DL,Schwager A,Abdullah O,Ritchey JW,Fung KM,Saunders D,Jensen RL,Towner RA

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunohistochemistry

Tue Dec 01 00:00:00 EST 2015

Molecular targeting of protein arginine deiminases to suppress colitis and prevent colon cancer.

Oncotarget

Witalison EE,Cui X,Causey CP,Thompson PR,Hofseth LJ

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunohistochemistry

Tue Nov 03 00:00:00 EST 2015

Molecular targeting of protein arginine deiminases to suppress colitis and prevent colon cancer.

Oncotarget

Witalison EE,Cui X,Causey CP,Thompson PR,Hofseth LJ

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunohistochemistry

Tue Nov 03 00:00:00 EST 2015

Amiodarone as an autophagy promoter reduces liver injury and enhances liver regeneration and survival in mice after partial hepatectomy.

Scientific reports

Lin CW,Chen YS,Lin CC,Chen YJ,Lo GH,Lee PH,Kuo PL,Dai CY,Huang JF,Chung WL,Yu ML

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunohistochemistry

Fri Oct 30 00:00:00 EDT 2015

Amiodarone as an autophagy promoter reduces liver injury and enhances liver regeneration and survival in mice after partial hepatectomy.

Scientific reports

Lin CW,Chen YS,Lin CC,Chen YJ,Lo GH,Lee PH,Kuo PL,Dai CY,Huang JF,Chung WL,Yu ML

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunohistochemistry

Fri Oct 30 00:00:00 EDT 2015

Novel In Vivo model for combinatorial fluorescence labeling in mouse prostate.

The Prostate

Fang X,Gyabaah K,Nickkholgh B,Cline JM,Balaji KC

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunofluorescence

Mon Jun 15 00:00:00 EDT 2015

Genetic disruption of Smad7 impairs skeletal muscle growth and regeneration.

The Journal of physiology

Cohen TV,Kollias HD,Liu N,Ward CW,Wagner KR

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunofluorescence

Mon Jun 01 00:00:00 EDT 2015

Regulation of YAP by mTOR and autophagy reveals a therapeutic target of tuberous sclerosis complex.

The Journal of experimental medicine

Liang N,Zhang C,Dill P,Panasyuk G,Pion D,Koka V,Gallazzini M,Olson EN,Lam H,Henske EP,Dong Z,Apte U,Pallet N,Johnson RL,Terzi F,Kwiatkowski DJ,Scoazec JY,Martignoni G,Pende M

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunohistochemistry

Mon Oct 20 00:00:00 EDT 2014

Darpp-32 and t-Darpp are differentially expressed in normal and malignant mouse mammary tissue.

Molecular cancer

Christenson JL,Kane SE

PA5-19462 was used in immunohistochemistry - paraffin section to discuss the contributions of Darpp-32 and t-Darpp to the development of breast cancer.

Fri Aug 15 00:00:00 EDT 2014

Darpp-32 and t-Darpp are differentially expressed in normal and malignant mouse mammary tissue.

Molecular cancer

Christenson JL,Kane SE

PA5-19462 was used in immunohistochemistry - paraffin section to discuss the contributions of Darpp-32 and t-Darpp to the development of breast cancer.

Fri Aug 15 00:00:00 EDT 2014

Development and application of specific cytokine assays in tissue samples from a bottlenose dolphin with hyperinsulinemia.

Frontiers in endocrinology

Eberle KC,Waters TE,Jensen ED,Venn-Watson SK,Sacco RE

Published figure using Ki-67 polyclonal antibody (Product # PA5-19462) in Immunofluorescence

Tue Oct 08 00:00:00 EDT 2013

Functional and mechanistic exploration of an adult neurogenesis-promoting small molecule.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Petrik D,Jiang Y,Birnbaum SG,Powell CM,Kim MS,Hsieh J,Eisch AJ

PA5-19462 was used in immunohistochemistry to study the mechanism by which a synthetic small molecule enhances neurogenesis and hippocampal function in adult animals

Wed Aug 01 00:00:00 EDT 2012

β-catenin/cyclin D1 mediated development of suture mesenchyme in calvarial morphogenesis.

BMC developmental biology

Mirando AJ,Maruyama T,Fu J,Yu HM,Hsu W

PA5-19462 was used in immunohistochemistry to investigate the role of beta-catenin/cyclin D1 during calvarial morphogenesis

Fri Nov 26 00:00:00 EST 2010

Bioinformatics

Protein Aliases: Antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; marker of proliferation Ki-67; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2

Gene Aliases: D630048A14Rik; I79_022666; Ki-67; Ki67; KIA; MIB-; MIB-1; MKI67; PPP1R105

UniProt ID: (Human) P46013, (Mouse) E9PVX6

Entrez Gene ID: (Dog) 100686578, (Pig) 102164100, (Sheep) 101106643, (Human) 4288, (Rabbit) 100343428, (Horse) 100055048, (Rat) 291234, (Chinese hamster) 100756884, (Mouse) 17345