Invitrogen

Ki-67 Polyclonal Antibody

Product Image Gallery

Ki-67 Antibody — Fig. 3 Co-ordinated expression of the non-LEE-encoded effector repertoire in vivo. a Expression dynamics of the NLE repertoire in vivo compared to in vitro growth in DMEM. The line plots illustrate fold-change in expression at each site and time point during infection. A selection of NLEs ( espS, espI nleA , espO, espM3 and espK ) displaying the highest fold changes in expression are labelled and colour coded while remaining NLE genes are illustrated in grey. Gene expression data represents the mean of three replicates in all cases and full expression heatmaps are found in Supplementary Figure 4a . b Venn diagrams illustrating the overlap in NLEs differentially expressed throughout infection ( P <= 0.05; EDGE test). NLEs that were significantly differentially expressed in all in vivo samples are colour coded as in panel 3a. c Enumeration of colonic epithelial cell crypt length (um) from mice (groups of n = 10; +- SEM) infected with either wild type C. rodentium , espS or uninfected controls. Sampling was carried out at peak infection (day eight PI). Representative images of H and E-stained colon sections from wild type or espS -infected mice are presented additionally. d Percentage measurement of epithelial proliferation in response to wild type or espS infection by Ki-67 staining (+-SEM). Representative images of stained colon sections from wild type or espS -infected mice are presented. Markers used stained for C . rodentium (anti-Int280beta; Cy3 red),

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1:100

Immunocytochemistry (ICC)

0.5-1 µg/ml

Immunofluorescence (IF)

0.5-1 µg/mL

Immunohistochemistry - Free Floating (IHC (Free))

1:500

Immunohistochemistry (Paraffin) (IHC (P))

0.1 - 5 µg/mL

Published Applications

Immunofluorescence (IF)

See 7 publications below

Immunohistochemistry (IHC)

See 13 publications below

Miscellaneous PubMed (MISC)

See 4 publications below

Immunohistochemistry (Paraffin) (IHC (P))

See 4 publications below

Immunohistochemistry (Frozen) (IHC (F))

See 2 publications below

Product Specifications

Species Reactivity

Bovine, Dog, Deer, Horse, Hamster, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat

Published species

Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 1200 - 1300 of Human Ki67.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_10981523

Product Specific Information

Heat mediated antigen retrieval recommended prior to tissue staining.

Store antibody at 4°C for 1-2 weeks. For long-term storage, store at -20°C.

Target Information

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; marker of proliferation Ki-67; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2, KIA, antigen KI-67

Gene Aliases: D630048A14Rik; I79_022666; Ki-67; Ki67; KIA; MIB-; MIB-1; MKI67; PPP1R105

UniProt ID: (Human) P46013, (Mouse) E9PVX6

Entrez Gene ID: (Dog) 100686578, (Sheep) 101106643, (Human) 4288, (Rat) 291234, (Chinese hamster) 100756884, (Mouse) 17345

Function(s)

DNA metabolic process meiotic nuclear division cell proliferation response to organic cyclic compound hyaluronan metabolic process organ regeneration cellular response to heat hepatocyte proliferation cholangiocyte proliferation

Process(es)

protein binding ATP binding protein C-terminus binding poly(A) RNA binding molecular_function