Invitrogen

Ki-67 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View Details

31 Published Figures

50 References

View all (119) Ki-67 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

Ki-67 Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of Ki67 was performed using 70% confluent log phase HeLa cells serum starved for 36 Hrs followed by serum release for 6 Hrs. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Ki-67 Polyclonal Antibody (Product # PA5-19462) at 1 ug/ml in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents serum starved cells with reduced signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Ki-67 Antibody in Cell treatment — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis using Anti-Ki-67 Polyclonal Antibody (Product # PA5-19462) shows increased expression of proteins upon serum starvation followed by serum release in HeLa cells. {TM}

Ki-67 Antibody — Figure 1 ( a ) Tumor growth rate after the subcutaneous injection of B16-F10 cells in control (Crl), vehicle-(Veh), propranolol-(Prop), or SR59230A-(SR) treated C57BL/6 mice (n = 6). Values are presented as mean +- SD. ( b ) Representative Annexin V/PI staining plots of homogenized tumor samples after 20 days of treatment (T20). ( c ) Confocal microscopy images of PI staining of tumors obtained from mice injected with B16-F10-GFP. ( d ) Representative immunohistochemical staining of proliferation marker Ki-67 in tumor tissues after 20 days of treatment (T20), and relative quantification. ( e ) Representative immunohistochemical staining of beta2- and beta3-ARs in tumor tissues, and relative quantification after 10 days (T10) and 20 days (T20) of treatment. ns= not significant; beta2-Ars = beta2-Adrenoreceptors; beta3-ARs= beta3-Adrenoreceptors.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

0.5-1 µg/ml

View 2 publications 2 publications

Immunofluorescence (IF)

0.5-1 µg/mL

View 13 publications 13 publications

Immunohistochemistry (Paraffin) (IHC (P))

0.1 - 5 µg/mL

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 25 publications 25 publications

Miscellaneous PubMed (Misc)

View 4 publications 4 publications

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Product Specifications

Species

Bovine, Dog, Horse, Hamster, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat

Published species

Artificial Control, Human, Mouse, Rat

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 1200 - 1300 of Human Ki67.

Conjugate

Unconjugated

Form

Liquid

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_10981523

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to tissue staining.

Recommended positive controls:

ICC/IF - Wildtype HAP1 cells, SK-N-SH cells, HeLa cells, MEF1 cells

IHC (P) - Mouse and human spleen tissue, human skin carcinoma tissue, human colon tissue, mouse tumor tissue, human skin tissue

Target Information

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; marker of proliferation Ki-67; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2

Gene Aliases: D630048A14Rik; I79_022666; Ki-67; Ki67; KIA; MIB-; MIB-1; MKI67; PPP1R105

UniProt ID: (Human) P46013, (Mouse) E9PVX6

Entrez Gene ID: (Dog) 100686578, (Pig) 102164100, (Sheep) 101106643, (Human) 4288, (Rabbit) 100343428, (Horse) 100055048, (Rat) 291234, (Chinese hamster) 100756884, (Mouse) 17345