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Rockland
Kmo Polyclonal Antibody
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FIGURE: 1 / 1
Kmo Antibody (600-401-J26) in WB
Western Blot of Rabbit anti-KMO antibody. Lane 1: Brain Extract. Load: 10 µg per lane. Primary antibody: KMO antibody at 1µg/mL for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 58 kDa for KMO. Other band(s): Other bands at lower molecular weights. These bands are all specifically blocked by KMO peptide.
Product Details
600-401-J26
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KMO affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding 200-300aa of the mouse KMO chain.
Conjugate
Form
Liquid
Concentration
1.07 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 50% glycerol, 0.15M NaCl
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-KMO specific towards Kynurenine 3-monooxygenase (KMO). A BLAST analysis was used to suggest cross-reactivity with Mouse based on 100% sequence homology. Cross-reactivity with KMO from other sources has not been determined.
Target Information
Kynurenine 3-monooxygenase (KMO; EC 1.14.13.9) is an NADPH-dependent flavin monooxygenase that catalyzes the hydroxylation of the L-tryptophan metabolite L-kynurenine to form L-3-hydroxykynurenine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: kmo; Kynurenine 3-hydroxylase; Kynurenine 3-monooxygenase
Gene Aliases: AI046660; Kmo
UniProt ID: (Mouse) Q91WN4
Entrez Gene ID: (Mouse) 98256
tryptophan catabolic process
metabolic process
response to salt stress
pyridine nucleotide biosynthetic process
tryptophan catabolic process to kynurenine
NAD metabolic process
quinolinate biosynthetic process
secondary metabolite biosynthetic process
oxidation-reduction process
kynurenine metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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