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Invitrogen
LAMP-2A Polyclonal Antibody (AMC2)
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FIGURE: 1 / 59
LAMP-2A Antibody (51-2200) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of C2C12 (Lane 1), NIH/3T3 (Lane 2) and PC-12 (Lane 3). The blot was probed with Anti-LAMP2 Rabbit Polyclonal Antibody (Product # 51-2200, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjµgate (Product # A27036, 0.4µg/mL, 1:2500 dilution). A ~110 kDa band corresponding to LAMP2 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPA... View More
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.jpg?time=20220826?time=20220826 "LAMP-2A Antibody (51-2200) in IHC")
.jpg?time=20220826?time=20220826 "LAMP-2A Antibody (51-2200) in IHC")
.jpg?time=20220826?time=20220826 "LAMP-2A Antibody (51-2200) in WB")
.jpg?time=20220826?time=20220826 "LAMP-2A Antibody (51-2200) in WB")
Product Details
51-2200
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
C. elegans,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Clone
AMC2
Immunogen
A synthetic peptide of the cytosolic tail of rat LAMP-2A (GLKRHHTGYEQF)
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody was tested for specificity in rat and mouse (it does not cross-react with the human variant) and was demonstrated to be highly selective for LAMP-2A without cross-reacting with LAMP-2B or C. This protein was previously known as lgp96.
In western blotting this antibody was used at 90 ng/mL with 100 µg of rat liver lysosomal protein. This antibody is also able to completely block transport of cytosolic proteins into lysosomes in rat-derived cells.
Target Information
LAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins. LAMP2 provides selectins with carbohydrate ligands, and may play a role in tumor cell metastasis, and have a function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is approximately 46 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of about 100-130 kDa. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. LAMP2 is used to monitor a very selective form of autophagy called chaperone-mediated autophagy. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. The LAMP protein has 3 variant forms: LAMP-2A, -2B, and 2C. LAMP2, together with CD107a/LAMP-1, is a major constituent of lysosomal membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes, and LAMP2 is an important regulator in successful phagosomal maturation. LAMP2 deficiency causes an accumulation of autophagosomes in many tissues leading to cardiomyopathy and myopathy (Danon's disease).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD107 antigen-like family member B; CD107b; Igp96; LAMP-2; LAMP-2 Isoform 2A; LAMP-2 Variant A; LGP-110; LGP-96; LGP-B; lysosomal membrane glycoprotein 2; Lysosomal membrane glycoprotein type B; Lysosome-associated membrane glycoprotein 2; lysosome-associated membrane protein 2; RP23-193O17.2
Gene Aliases: CD107b; Lamp II; Lamp-2; Lamp-2a; Lamp-2b; Lamp-2c; Lamp2; LGP-B; Mac3
UniProt ID: (Mouse) P17047, (Rat) P17046
Entrez Gene ID: (Mouse) 16784, (Rat) 24944
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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