|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C K(407) R S H A G Y Q T I(416) of human LAMP1.|
|Storage buffer||0.02M tris, pH 7.4, with 0.1M glycine|
|Contains||0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay dependent|
|Western Blot (WB)||1:250 - 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 5 publications below|
PA1-654A detects Lamp1 from human samples.
PA1-654A has been successfully used in immunofluorescence and Western blotting procedures. In immunofluorescence procedures, this antibody stains Lamp1 in HeLa cells resulting in perinuclear staining.
The PA1-654A immunogen is a synthetic peptide corresponding to residues C K(407) R S H A G Y Q T I(416) of human LAMP1. This region is completely conserved between human, mouse and rat.
Lysosomes are membrane-bound vacuoles which play a critical role in cellular metabolism. These organelles participate in endocytosis, the synthesis and assembly of acid hydrolases, act as sites for digestion of foreign materials and for specialized autolytic cellular processes. Membrane-associated glycoproteins have an important role in normal lysosomal function. Lamp1, also known as lysosome associated membrane protein 1, is a heavily glycosylated membrane protein of the lysosome. It contains a putative signal peptide, 18 sites for N-linked glycosylation, a single membrane-spanning segment and a short (11 amino acid) cytosolic tail.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The PKD domain distinguishes the trafficking and amyloidogenic properties of the pigment cell protein PMEL and its homologue GPNMB.
PA1-654A was used in immunocytochemistry to study the role of the PKD domain and its gycosylation in the differences in trafficking and amyloidogenesis observed between PMEL and GPNMB
|Theos AC,Watt B,Harper DC,Janczura KJ,Theos SC,Herman KE,Marks MS||Pigment cell and melanoma research (26:470)||2013|
NADPH oxidase NOX2 defines a new antagonistic role for reactive oxygen species and cAMP/PKA in the regulation of insulin secretion.
PA1-654A was used in immunocytochemistry to study the role of cAMP/PKA in the mechanism by which NOX2-generated ROS impair insulin secretion
|Li N,Li B,Brun T,Deffert-Delbouille C,Mahiout Z,Daali Y,Ma XJ,Krause KH,Maechler P||Diabetes (61:2842)||2012|
Retromer disruption promotes amyloidogenic APP processing.
PA1-654A was used in immunocytochemistry to investigate the effect of retromer malfunction on amyloidogenic APP processing
|Sullivan CP,Jay AG,Stack EC,Pakaluk M,Wadlinger E,Fine RE,Wells JM,Morin PJ||Neurobiology of disease (43:338)||2011|
Actin cytoskeleton-dependent Rab GTPase-regulated angiotensin type I receptor lysosomal degradation studied by fluorescence lifetime imaging microscopy.
PA1-654A was used in immunocytochemistry to investigate the angiotensin type 1 receptor subcellular trafficking and degradation
|Li H,Yu P,Sun Y,Felder RA,Periasamy A,Jose PA||Journal of biomedical optics (15:null)||2010|
VCP/p97 is essential for maturation of ubiquitin-containing autophagosomes and this function is impaired by mutations that cause IBMPFD.
PA1-654A was used in immunocytochemistry to investigate the role of VCP/p97 on the maturation of autophagosome
|Tresse E,Salomons FA,Vesa J,Bott LC,Kimonis V,Yao TP,Dantuma NP,Taylor JP||Autophagy (6:217)||2010|