Immunofluorescent analysis of LAMP-2 (green) in NIH/3T3 cells. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 1% Blocker BSA (Product # 37525), each for 15 minutes at room temperature. Cells were stained with a LAMP-2 polyclonal antibody (Product # PA1-655) at a concentration of 20ug/ml in 1% Blocker BSA in PBS (right panel), or incubated in blocking buffer alone as a negative control (left panel) overnight at 4C, and then incubated with a Dylight 488-conjugated goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:1000 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI (Product # 46190). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: C G(400) L K R H H T G Y E Q F(411)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay dependent|
|Western Blot (WB)||1:100 - 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-655 detects lysosome-associated membrane protein-2 (LAMP2) from rat, mouse and human tissues.
PA1-655 has been successfully used in Western blot, immunofluorescence, and immunohistochemistry. By Western blot, this antibody detects glycosylated and non-glycosylated LAMP-2, depending on the lysis buffer used.
PA1-655 immunizing peptide corresponds to amino acid residues 400-411 from rat LAMP2. This sequence is completely conserved between rat, mouse, and guinea pig LAMP2. PA1-655 immunizing peptide (Cat. # PEP-039) is available for use in neutralization and control experiments.
Lysosomes are membrane-bound vacuoles which play a critical role in cellular metabolism. These organelles participate in endocytosis, the synthesis and assembly of acid hydrolases, act as sites for digestion of foreign materials and for specialized autolytic cellular processes. Membrane-associated glycoproteins have an important role in normal lysosomal function. Prior to posttranslational modification, Lysosome-associated membrane protein-2 (LAMP-2) is an ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of ~100-110 kDa. LAMP2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Low autophagy capacity implicated in motor system vulnerability to mutant superoxide dismutase.
PA1-655 was used in western blot to discuss how mutations in superoxide dismutase-1 affect the motor system
|Tokuda E,Brännström T,Andersen PM,Marklund SL||Acta neuropathologica communications (4:null)||2016|
Internalization and axonal transport of the HIV glycoprotein gp120.
PA1-655 was used in immunocytochemistry to investigate gp120 internalization and axonal transport in peripheral nervous system neurons
|Berth S,Caicedo HH,Sarma T,Morfini G,Brady ST||ASN neuro (7:null)||2015|
Syntenin-ALIX exosome biogenesis and budding into multivesicular bodies are controlled by ARF6 and PLD2.
PA1-655 was used in immunocytochemistry to study the roles of ARF6 and PLP2 in the biogenesis of exosomes
|Ghossoub R,Lembo F,Rubio A,Gaillard CB,Bouchet J,Vitale N,Slavík J,Machala M,Zimmermann P||Nature communications (5:null)||2014|
Inhibition of macroautophagy triggers apoptosis.
PA1-655 was used in immunocytochemistry to investigate the relationship between macroautophagy and apoptosis in mammalian cells.
|Boya P,González-Polo RA,Casares N,Perfettini JL,Dessen P,Larochette N,Métivier D,Meley D,Souquere S,Yoshimori T,Pierron G,Codogno P,Kroemer G||Molecular and cellular biology (25:1025)||2005|
Trafficking of exogenous peptides into proteasome-dependent major histocompatibility complex class I pathway following enterotoxin B subunit-mediated delivery.
PA1-655 was used in immunocytochemistry to investigate the trafficking pathway of EtxB-peptide conjugates.
|Hearn AR,de Haan L,Pemberton AJ,Hirst TR,Rivett AJ||The Journal of biological chemistry (279:51315)||2004|
Nonmuscle myosin II powered transport of newly formed collagen fibrils at the plasma membrane.
PA1-655 was used in immunohistochemistry to study the role of non-muscle myosin II in the plasma membrane transport of newly formed collagen fibrils
|Kalson NS,Starborg T,Lu Y,Mironov A,Humphries SM,Holmes DF,Kadler KE||Proceedings of the National Academy of Sciences of the United States of America (110:E4743)||2013|
Different steady state subcellular distributions of the three splice variants of lysosome-associated membrane protein LAMP-2 are determined largely by the COOH-terminal amino acid residue.
PA1-655 was used in immunohistochemistry to study the mechanism for the subcellular distributions of lysosome-associated membrane protein LAMP-2 splice variants
|Gough NR,Fambrough DM||The Journal of cell biology (137:1161)||1997|
Calpains mediate epithelial-cell death during mammary gland involution: mitochondria and lysosomal destabilization.
PA1-655 was used in western blot to study the role of mitochondria and lysosomes in calpain-mediated epithelial-cell death during mammary gland involution
|Arnandis T,Ferrer-Vicens I,García-Trevijano ER,Miralles VJ,García C,Torres L,Viña JR,Zaragozá R||Cell death and differentiation (19:1536)||2012|
Endocytosis in cultured neurons is altered by chronic alcohol exposure.
PA1-655 was used in western blot to investigate the influence of chronic alcohol consumption on neuronal endocytosis
|Marín MP,Esteban-Pretel G,Ponsoda X,Romero AM,Ballestín R,López C,Megías L,Timoneda J,Molowny A,Canales JJ,Renau-Piqueras J||Toxicological sciences : an official journal of the Society of Toxicology (115:202)||2010|
Identification and characterization of mature beta-hexosaminidases associated with human placenta lysosomal membrane.
PA1-655 was used in western blot to identify and characterize beta hexosaminidases
|Magini A,Mencarelli S,Tancini B,Ciccarone V,Urbanelli L,Hasilik A,Emiliani C||Bioscience reports (28:229)||2008|
Hsp70 protects against UVB induced apoptosis by preventing release of cathepsins and cytochrome c in human melanocytes.
PA1-655 was used in western blot to investigate the anti-apoptotic mechanism of Hsp70 in human melanocytes
|Bivik C,Rosdahl I,Ollinger K||Carcinogenesis (28:537)||2007|
MARCH-II is a syntaxin-6-binding protein involved in endosomal trafficking.
PA1-655 was used in western blot to identify membrane-associated RING-CH (MARCH) and its binding to syntaxin 6.
|Nakamura N,Fukuda H,Kato A,Hirose S||Molecular biology of the cell (16:1696)||2005|