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Invitrogen
LAMP2 Polyclonal Antibody
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FIGURE: 1 / 40
LAMP2 Antibody (PA1-655) in ICC/IF
Immunofluorescent analysis of LAMP-2 (green) in NIH/3T3 cells. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 1% Blocker BSA (Product # 37525), each for 15 minutes at room temperature. Cells were stained with a LAMP-2 polyclonal antibody (Product # PA1-655) at a concentration of 20 µg/mL in 1% Blocker BSA in PBS (right panel), or incubated in blocking buffer alone as a negative control (left panel) overnight at 4C, and then incubated with a Dylight 488-conjugated goat anti-rabbit Ig... View More
Product Details
PA1-655
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Chicken,
Goat,
Human,
Mouse,
Rabbit,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: C G(400) L K R H H T G Y E Q F(411)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-655 detects lysosome-associated membrane protein-2 (LAMP2) from rat, mouse and human tissues.
PA1-655 has been successfully used in Western blot, immunofluorescence, and immunohistochemistry. By Western blot, this antibody detects glycosylated and non-glycosylated LAMP-2, depending on the lysis buffer used.
PA1-655 immunizing peptide corresponds to amino acid residues 400-411 from rat LAMP2. This sequence is completely conserved between rat, mouse, and guinea pig LAMP2. PA1-655 immunizing peptide (Cat. # PEP-039) is available for use in neutralization and control experiments.
Target Information
LAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins. LAMP2 provides selectins with carbohydrate ligands, and may play a role in tumor cell metastasis, and have a function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is approximately 46 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of about 100-130 kDa. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. LAMP2 is used to monitor a very selective form of autophagy called chaperone-mediated autophagy. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. The LAMP protein has 3 variant forms: LAMP-2A, -2B, and 2C. LAMP2, together with CD107a/LAMP-1, is a major constituent of lysosomal membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes, and LAMP2 is an important regulator in successful phagosomal maturation. LAMP2 deficiency causes an accumulation of autophagosomes in many tissues leading to cardiomyopathy and myopathy (Danon's disease).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD107 antigen-like family member B; CD107b; Igp96; LAMP-2; LAMP-2 Isoform 2A; LAMP-2 Variant A; LAMP-2C; LGP-110; LGP-96; LGP-B; lysosomal membrane glycoprotein 2; Lysosomal membrane glycoprotein type B; Lysosome-associated membrane glycoprotein 2; lysosome-associated membrane protein 2; RP23-193O17.2; unnamed protein product
Gene Aliases: CD107b; DND; Lamp II; LAMP-2; Lamp-2a; Lamp-2b; Lamp-2c; LAMP2; LAMPB; LGP-96; LGP-B; LGP110; Mac3
UniProt ID: (Human) P13473, (Mouse) P17047, (Rat) P17046
Entrez Gene ID: (Human) 3920, (Mouse) 16784, (Rat) 24944
protein targeting
autophagy
lysosomal lumen acidification
cellular response to starvation
protein import
protein catabolic process
negative regulation of protein complex assembly
regulation of protein stability
lysosomal lumen pH elevation
muscle cell cellular homeostasis
protein stabilization
chaperone-mediated autophagy
protein targeting to lysosome involved in chaperone-mediated autophagy
autophagosome maturation
negative regulation of NLRP3 inflammasome complex assembly
hydrogen ion transmembrane transport
lysosomal protein catabolic process
biological_process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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